Studies on Physiological Effects and Plasmid Stability of JM109 Host System
Praveen Kumar Vemuri *
Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Andhra Pradesh, India.
Shaik Mohammad Anjum
Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Andhra Pradesh, India.
Lakshmi Saranya Medida
Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Andhra Pradesh, India.
P. V. Hemanth Sai
Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Andhra Pradesh, India.
Mahathi Gummavajjala
Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Andhra Pradesh, India.
Braja Kishori Panigrahi
Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Andhra Pradesh, India.
Swati Dash
Department of Genetic Engineering, SRM Institute of Science and Technology, Tamil Nadu, India.
Swati Senapati
Department of Biotechnology, SRM Institute of Science and Technology, Tamil Nadu, India.
*Author to whom correspondence should be addressed.
Abstract
Objective: The purpose of the study delineates the growth and plasmid stability of E. coli JM109 host system.
Methods: Different concentrations of drugs, chemicals and various frequency of radiations were subjected to the host system to verify the colony forming units along with plasmid concentration and stability.
Results: Among chemicals, acridine orange showed highest effect on growth of DH5a, while among the drugs, dantron showed maximum effect on the growth of the organism. Radio frequency of 2GHz and low intensity microwave radiation were recorded as highest inhibitory effects. However, there is no significant effect in growth was observed in exposure to UV rays. Conclusion: The present work discussed that, the effect of drugs, chemicals, radio frequency and microwave radiation have a huge effect not only on growth of organism but also concentration and stability of plasmid.
Keywords: JM109, dantron, acridine orange, radio frequency, microwave.