Biosynthesis of Hydroxy Citric Acid Mediated Zinc Nanoparticles and Its Antioxidant and Cytotoxic Activity
S. Barani Shankar
Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Science, Saveetha University, Chennai-77, Tamil Nadu, India.
Lakshminarayanan Arivarasu *
Department of Pharmacology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Science, Saveetha University Chennai-77, Tamil Nadu, India.
S. Rajeshkumar
Department of General Pharmacology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Science, Saveetha University Chennai-77, Tamil Nadu, India.
*Author to whom correspondence should be addressed.
Abstract
Aim: To evaluate biosynthesis of hydroxy citric acid mediated zinc nanoparticle and its antioxidant and cytotoxic activity
Introduction: Hydroxy citric acid is a chemical that is found in fruit rinds of Garcinia cambogia, Garcinia indica, and Garcinia atroviridis. Hydroxy citric acid is used to improve exercise performance and weight loss. Hydroxy citric acid can cause nausea, digestive tract discomfort, and headache when used short-term. Long-term safety is unknown.Zinc oxide nanoparticles are nanoparticles of zinc oxide that have diameters less than 100 nanometers. Zinc nanoparticles have a large surface area relative to their size and high catalytic activity. The exact physical and chemical properties of zinc oxide nanoparticles depend on the different ways they are synthesized. zinc oxide nanoparticles have also been shown to exhibit strong protein adsorption properties, which can be used to modulate cytotoxicity, metabolism or other cellular responses.
Materials and Methods: Evaluation of anti-oxidant was done by DPPH activity and evaluation of cytotoxic activity was done by brine shrimp lethality assay. Colour changes was noted in evaluation of cytotoxic activity
Results: The antioxidant activity was exhibited well at higher doses when compared to standard, but there was only 40% of cytotoxicity achieved which indicates its potential.Cytotoxic activity reveals that the drug showed a very good result at a concentration of 25 ul it showed a 40% of lethality thereby showing that Hydroxycitric acid medicated zinc oxide nanoparticles shows a potent activity when compared to that with the standard.
Conclusion: The study concludes that hydroxy citric acid shows good antioxidant and cytotoxic activity.
Keywords: Antioxidant, cytotoxicity, DPPH, garcinia, hydroxy citric acid, zinc oxide.