Bacterial Analysis for Seminal Fluid befor In-vitro Fertilization Procedure
S. O. Noor *
Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
A. Albalawi
Microbiology Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.
H. Abduljabbar
Department of Obstetrics and Gynecology, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia.
Ebtesam H. Alosaimi
Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
Sabah M. Hassan
Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia and Department of Genetics, Faculty of Agriculture, Ain Shams University, Cairo, Egypt.
A. Najjar
Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
*Author to whom correspondence should be addressed.
Abstract
In vitro fertilization (IVF) is the most advanced technique of assisted reproductive where the sperm reach the eggs for fertilization in a laboratory tube. Seminal fluid often contains bacterial contaminants that decrease the quality and quantity of seminal fluids.
Aim: In this study we investigated the presence of bacteria in semen before IVF procedure.
Methodology: All samples were identified according to the morphological, biochemical, molecular, and antimicrobial tests.
Results: Microbial species were detected in 28% of semen samples, while 72% did not show any bacterial growth. The prevalent bacterial isolates were Staphylococcus spp., Enterococcus spp., Catellicoccus spp., and Pseudomonas spp.
Conclusion: The use of microbiological analysis to identify resistant bacterial isolates is considered an effective method to reduce infertility in men by changing the contaminated semen.
Keywords: Seminal fluid, In vitro fertilization, semen, Infertility