Hyaluronic Acid Mediated Zinc Nanoparticles against Oral Pathogens and Its Cytotoxic Potential
V. Karthik
Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India.
Lakshminarayanan Arivarasu *
Department of Pharmacology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai-77, Tamil Nadu, India.
S. Rajeshkumar
Department of Pharmacology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai-77, Tamil Nadu, India.
*Author to whom correspondence should be addressed.
Abstract
Aim: To determine the Hyaluronic acid mediated zinc nanoparticles against oral pathogens and cytotoxic potential.
Introduction: Hyaluronic acid is a non sulfated glycosaminoglycan. Bacterial invasion can also be repressed by an inhibitor Interfering with receptor interaction for bacterial invasion, Hyaluronic acid is an example of inhibitor.Nanoparticles is considered as one of the most promising studies in science and technology study and Maintaining the shape, size and distribution of nanoparticles helps in its function and interaction with other molecules.
Materials and Methods: 0.1 g of Hyaluronic acid was added to a flask containing 100 ml of distilled water and heated for an hour. After observing the solubility, 0.574 g of Zn was added to the mixture and then kept on a magnetic stirrer for 1 hour at 100 degree Celsius.
Anti microbial activity: Agar well diffusion and Disc diffusion method is used. Then incubated for 37 degree Celsius for 48 hour. The zone of inhibition is recorded.
Cytotoxic potential: Different concentrations of Hyaluronic acid mediated zinc nanoparticles are incorporated to the wells. After 24 hrs the results were analysed.
Results and Discussion: Hyaluronic mediated zinc nanoparticles is proved to be effective against a wide range of foodborne and clinically relevant Gram-positive and Gram-negative bacteria using several assays such as disk diffusion, agar or broth dilution.Hyaluronic acid mediated Zinc nanoparticles has high potent cytotoxic potential it had been proved with the help of brine shrimps.
Conclusion: From the observed results, it has been concluded that Hyaluronic acid has a lot of medicinal values and it has antimicrobial activity and it has good cytotoxic potential.
Keywords: Hyaluronic acid, antimicrobial, cytotoxicity, zinc oxide, nanoparticle, brine shrimp