Effect of Methanolic Extract of Securigera securidaca as Antioxidant and Antibacterial Activities
Ghassab M. Al- Mazaideh *
Department of Chemistry and Chemical Technology, Faculty of Science, Tafila Technical University, P.O.Box 179, Tafila 66110, Jordan.
Saleh A. Al- Quran
Department of Biological Sciences, Faculty of Science, Mutah University, P.O.Box 7, Mutah 61710, Al-Karak, Jordan.
*Author to whom correspondence should be addressed.
Abstract
In the present work, the phytochemical screening, polyphenolic content, antibacterial activity and antioxidant activity of Securigera securidaca seeds in methanol were carried out. Phytochemical analysis of seeds showed the presence of alkaloids, flavonoids, saponins, terpenoids, steroids and glycosides. Total phenolic content was estimated by Folin Ciocalteau method and the result showed the highest phenolic content of 62.28 mg/g. Methanolic extract was screened for antibacterial activity by disc diffusion method and it found to be potent. The MIC of methanol extract identified by broth dilution method showed a MIC value of 0.25 mg/ml for both E. coli and Kl. Oxytoca, and also 0.5 mg/ml for both S. aureus and S. epidermis. The antioxidant effect of the seeds was tested by DPPH scavenging activity as in vitro assay. The extract had potent inhibitory activity (IC50) value of 0.057 mg/ml. The finding experimental results showed that methanolic extract of Securigera securidaca is important as a source of antibacterial activity and polyphenolic antioxidants.
Keywords: Securigera securidaca, antioxidant, anticorrosion, polyphenolic content, MIC