Molecular Typing of Brucella melitensis Isolated from Patients and Animals by Pulsed Field Gel Electrophoresis from Iran

Nasrin Bahmani

Brucellosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.

Mohammad Reza Arabestani

Brucellosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.

Seyed Hamid Hashemi

Brucellosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.

Abbas Farahani

Department of Microbiology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Reza Mirnejad

Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.

Parviz Mohajeri

Department of Microbiology, Kermanshah University of Medical Sciences, Kermanshah, Iran.

Manoochehr Karami

Research Center for Health Sciences, Hamadan University of Medical Sciences, Hamadan, Iran.

Mohammad Yousef Alikhani *

Brucellosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran and Department of Microbiology, Hamadan University of Medical Sciences, Hamadan, Iran.

*Author to whom correspondence should be addressed.


Abstract

Background: The Brucellosis is recognized as a significant public health problem, with major economic and financial burdens in countries where the disease remains endemic. The disease is one of the most important worldwide zoonosis affecting livestock and humans. The aim of the present study was to genotypically characterize Brucella strains isolated from human and animal samples in two provinces of Iran.

Methods: Twenty seven Brucella strains isolated from patients and animals during April 2015 - December 2016. Thirteen human and fourteen animal strains were identified by biochemical tests and confirmed by amplification of fragment 1100bp omp2a gene using Polymerase Chain Reaction (PCR) method. Isolates were genotypically characterized by XbaI digestion and pulsed-field gel electrophoresis (PFGE) technique.

Results: In PFGE analysis totally, 7 common clones and 3 single clones were obtained. In genotyping, in two clones/clusters, human isolates were grouped with genotypes from animal isolates. Also PFGE results indicated 21 Brucella strains an overall similarity higher than 90%, and three clusters based on 100% similarity were revealed.

Conclusion: According to dendrogram clinical strains had a high degree of homology compared to animal strains. The information of this study indicating a close genetic relatedness or common origin of the isolates in the two geographical region of Iran and it implies that B. melitensis cross-infected between human and livestock.

Keywords: Brucella melitensis, molecular typing, PFGE


How to Cite

Bahmani, N., Arabestani, M. R., Hashemi, S. H., Farahani, A., Mirnejad, R., Mohajeri, P., Karami, M. and Alikhani, M. Y. (2017) “Molecular Typing of Brucella melitensis Isolated from Patients and Animals by Pulsed Field Gel Electrophoresis from Iran”, Journal of Pharmaceutical Research International, 18(4), pp. 1–9. doi: 10.9734/JPRI/2017/34911.