Antiglycation and Fatty Acids Profiling in Response to Phyocyanin Extracted from Chlorophyta Ulva lactuca Algae Loaded on Albumin Nano-particles (ULANP) in Diabetic Rats
Abdulrahman L. Al-Malki *
Department of Biochemistry, Faculty of Science, King Abdulaziz University (KAU), P.O.Box 80203, Jeddah, Saudi Arabia and Bioactive Natural Products Research Group, KAU, Saudi Arabia and Experimental Biochemistry Unit, King Fahd Medical Research Center, KAU, Saudi Arabia.
Elie K. Barbour
Experimental Biochemistry Unit, King Fahd Medical Research Center, KAU, Saudi Arabia and Department of Agriculture, Faculty of Agricultural and Food Sciences, American University of Beirut, Beirut, Lebanon and Department of Adjuncted to Biochemistry, Faculty of Science, KAU, Saudi Arabia.
Khadijah S. Balamash
Department of Biochemistry, Faculty of Science, King Abdulaziz University (KAU), P.O.Box 80203, Jeddah, Saudi Arabia.
Fawzia A. Alshubaily
Department of Biochemistry, Faculty of Science, King Abdulaziz University (KAU), P.O.Box 80203, Jeddah, Saudi Arabia.
Khalid O. Abualnaja
Department of Biochemistry, Faculty of Science, King Abdulaziz University (KAU), P.O.Box 80203, Jeddah, Saudi Arabia and Bioactive Natural Products Research Group, KAU, Saudi Arabia and Experimental Biochemistry Unit, King Fahd Medical Research Center, KAU, Saudi Arabia.
Wesam H. Abdulaal
Department of Biochemistry, Faculty of Science, King Abdulaziz University (KAU), P.O.Box 80203, Jeddah, Saudi Arabia and Department of Biochemistry, Cancer Metabolism and Epigetic Unit, KAU, Saudi Arabia.
Mustafa A. Zeyadi
Department of Biochemistry, Faculty of Science, King Abdulaziz University (KAU), P.O.Box 80203, Jeddah, Saudi Arabia.
Maryam H. Al-Zahrani
Department of Biochemistry, Faculty of Science, King Abdulaziz University (KAU), P.O.Box 80203, Jeddah, Saudi Arabia.
Taha A. Kumosani
Department of Biochemistry, Faculty of Science, King Abdulaziz University (KAU), P.O.Box 80203, Jeddah, Saudi Arabia and Experimental Biochemistry Unit, King Fahd Medical Research Center, KAU, Saudi Arabia and Production of Natural Products for Industrial Applications Research Group, KAU, Saudi Arabia.
Said S. Moselhy
Department of Biochemistry, Faculty of Science, King Abdulaziz University (KAU), P.O.Box 80203, Jeddah, Saudi Arabia and Bioactive Natural Products Research Group, KAU, Saudi Arabia and Experimental Biochemistry Unit, King Fahd Medical Research Center, KAU, Saudi Arabia and Department of Biochemistry, Faculty of Science, Ain Shams University, Egypt.
*Author to whom correspondence should be addressed.
Abstract
Aim: This study evaluated the effect of Phyocyanin extracted from Chlorophyta Ulva lactuca algae loaded on albumin nano-particles (ULANP) on diabetic rats.
Materials and Methods: Fifty albino rats were divided into 5 goups. GPI: control and GPII: rats were injected with alloxan (75 mg /kg) i.p for six consecutive days for induction of diabetes. This group was subdivided into 4 subgroups: GP IIa: (Untreated diabetic): GP IIb: rats were given with ULANP (100 mg/kg).GP IIc: Rats were given ULANP (200 mg/kg) i.p. GP IId: Rats were given insulin (100 unit/ day). Serum NO, interleukin-6 glucose, AGEs and fatty acids profile was determined.
Results: Analysis of ULANP by FTIR showed the characteristic band (2100 cm-1~ 3700 cm-1) that is indicated mainly from -COO, – CO and conjugated double bond. These bonds showed spectral bands peak 2985 cm-1 and 2860 cm-1, 2986 cm-1. Administration of ULANP in diabetic rats exerted an anti-inflammatory by lowering NO and IL-6 levels and hypoglycemic effects by decreased glucose and reduced AGEs levels. In addition, ULAPN lowered percent of saturated fatty acids while elevated unsaturated fatty acids percent.
Conclusion: It was concluded that, ULAPN is a promising effective anti-inflammatory and hypoglycemic agent compared with other therapeutic agents with lower site effects.
Keywords: Antiglycation, fatty acids, phyocyanin, Chlorophyta Ulva lactuca algae nano-particles, rats