The Effect of Methanolic Leaf Extract of Boerhavia diffusa Linn. (Nictaginaceae) on the Activities of Antidiabetic, Anti-inflammatory and Antioxidant Enzymes in Experimental Diabetes
Mama Sirou Amidou Ibrahima
Laboratoire de Physiopathologie Moléculaire et Toxicologie, Department of Animal Physiology, Faculty of Science and Technology, University of Abomey-Calavi, 01 BP: 4521 Cotonou, Benin.
Attakpa Sèlidji Eugène *
Laboratoire de Physiopathologie Moléculaire et Toxicologie, Department of Animal Physiology, Faculty of Science and Technology, University of Abomey-Calavi, 01 BP: 4521 Cotonou, Benin.
Béhanzin Gbèssohélè Justin
Laboratoire de Physiopathologie Moléculaire et Toxicologie, Department of Animal Physiology, Faculty of Science and Technology, University of Abomey-Calavi, 01 BP: 4521 Cotonou, Benin.
Amoussa Abdou Madjid
Unité de Biochimie et de Biologie Moléculaire, Laboratory of Biochemistry and Bioactives Natural Substances, Faculty of Science and Technology, University of Abomey-Calavi, 01 BP: 4521 Cotonou, Benin.
Sangaré Maxime Machioud
Laboratoire de Physiopathologie Moléculaire et Toxicologie, Department of Animal Physiology, Faculty of Science and Technology, University of Abomey-Calavi, 01 BP: 4521 Cotonou, Benin.
Akotegnon Rodrigue
Laboratoire de Physiopathologie Moléculaire et Toxicologie, Department of Animal Physiology, Faculty of Science and Technology, University of Abomey-Calavi, 01 BP: 4521 Cotonou, Benin.
Guinnin Félix
Laboratoire de Physiopathologie Moléculaire et Toxicologie, Department of Animal Physiology, Faculty of Science and Technology, University of Abomey-Calavi, 01 BP: 4521 Cotonou, Benin.
Lagnika Latifou
Unité de Biochimie et de Biologie Moléculaire, Laboratory of Biochemistry and Bioactives Natural Substances, Faculty of Science and Technology, University of Abomey-Calavi, 01 BP: 4521 Cotonou, Benin.
Sezan Alphonse
Laboratoire de Biomembranes et Signalisation Cellulaire, Department of Animal Physiology, Faculty of Science and Technology, University of Abomey-Calavi, 01 BP: 4521 Cotonou, Benin.
Baba-Moussa Lamine
Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculty of Science and Technology, University of Abomey-Calavi, 05 BP 1604 Cotonou, Benin.
Bialli Seri
Laboratoire de Neurosciences, Unité de Formation Biosciences, Université de Cocody-Abidjan, 22 BP 582, Abidjan 22, Côte-d’Ivoire.
Naim Akhtar Khan
INSERM U866, Physiologie de la Nutrition and Toxicologie, Université de Bourgogne, 6, Boulevard Gabriel, U.F.R Sciences de la Vie, Dijon 21000, France.
*Author to whom correspondence should be addressed.
Abstract
Diabetic therapeutic potentiality of methanol extract of stem leaves of Boerhavia diffusa was investigated following in-vivo study models in streptozotocin-induced diabetic rat. Methanol extract of stem leaves of Boerhavia diffusa exerted the glucose lowering effect an increase in serum insulin level on 28st day of postadministration. In addition to a higher expression of insulin receptor A. The extract treatment or glibenclamide for 28 days significantly (p<0.05) reduced HbA1c.
Boerhavia diffusa L. or glibenclamide for 28 days show no damaging effect on red blood count and hemoglobin when compared to the control group. Significant (p<0.05) increase in platelet count and white blood cell count was observed in groups treated with Boerhavia diffusa L. and glibenclamide when compared to control group.
Boerhavia diffusa L. and glibenclamide showed significant (P<0.05) decrease in total cholesterol (TC), triglyceride (TG) low density Lipoprotein (LDL), Alanine amino transferase (ALT), Aspartate amino transferase (AST), Alkaline phosphatase (ALP) and Gamma glutamyl transferase (GGT). Methanol extract of 600 mg/kg b.w had more lowering effect (p<0.05) on TC and TG as opposed to the untreated group.
Methanol extract or glibenclamide also modulated significantly (P<0.05) the activities of carbohydrate-metabolising enzymes and Hepatic glycogen content. Boerhavia diffusa or glibenclamide administration up-regulated mRNA expression of Glucose Transporter-2 (Glut2)
Boerhavia diffusa or glibenclamide also corrected antioxidant status of diabetic animals in liver. The lipid peroxidation inhibition activity of extracts from Boerhavia diffusa is stronger when compared to the reference antioxidant ascorbic acid.
These clearly showed that methanol extract from Boerhavia diffusa has the inhibitory activities of the xanthine oxidase, lipoxygenase and acetylcholinesterase enzyme.
Keywords: Boerhavia diffusa Linn, streptozotocin, diabetes mellitus, anti-inflammatory activity, antioxidant enzymes