Open Access Original Research Article

Simultaneous Estimation of Bilastine and Montelukast in Bulk by Rp-hplc and Assessment of Its Applicability in Marketed Tablet Dosage Form

Swati M. Andhale, Anna Pratima G. Nikalje

Journal of Pharmaceutical Research International, Page 8-25
DOI: 10.9734/jpri/2022/v34i3B35388

Aim: This study proposes to develop and validate the RP-HPLC method for Bilastine and Montelukast and to substantiate the RP-HPLC analysis bestowing to ICH validation guideline Q2R1.

Place and Duration of Study: Y. B. Chavan College of Pharmacy, Aurangabad, MS, India, between January 2020 and October 2021.

Methodology: The mixture of drugs was subjected to optimization by trial runs with different chromatographic parameters, viz. flow rate, λ in nm, etc. The system suitability was performed by repeated injections of Bilastine (200µg/mL) and Montelukast (200µg/mL) to confirm the optimization. Furthermore, the demonstrated method was validated as per ICH Q2R1 recommendations for parameters like accuracy, precision, robustness, the limit of detection and quantitation, etc.

Results: The outcomes of the method in terms of percent relative standard deviation (%RSD) of retention time (RT) and mean peak area were seen as 0.09, 0.35, and 0.35, 0.56 for Bilastine and Montelukast, respectively. The method was successful in achieving the qualifying criteria entrusted to ICH guidelines. The correlation coefficient, slope, and y-intercept were illustrated to be 0.9971, 17595, 217883, 0.998, 35458, and 17147, correspondingly for Bilastine and Montelukast, respectively. The range was seen in the order of 160-260µg/mL and 80-130µg/mL for Bilastine and Montelukast. The precision of the method was established with %RSD of repeatability and intermediate precision was < 2 at three standard levels across the range. The %accuracy of the method was observed in the range of 96.95-101.41 %w/w and 97.37-101.89 %w/w in the order for Bilastine and Montelukast. The robustness of the method displayed the results within the prescribed boundaries. The recovered amount of Bilastine and Montelukast by spike method was observed to be 96.37-98.88 %w/w and 96.11-100.06%w/w.

Conclusion: The author has accomplished the predefined goals by successful development and validation of the RP-HPLC method for the quantification of Bilastine and Montelukast as per ICH Q2R1 guidelines.

Open Access Original Research Article

Phytochemical, UV-Visible and FTIR Assessment along with in vitro Antioxidant Activity of Methanolic Extract of Tephrosia purpurea Linn Root

Pranita S. Jirvankar, D. Khobragade, S. Chandewar, A. Pimpale, R. Gawali, A. Lokade, A. Maske, R. Agrawal

Journal of Pharmaceutical Research International, Page 26-33
DOI: 10.9734/jpri/2022/v34i3B35389

Aims: The original phytochemical, UV-Visible, and FTIR Spectral estimation of Tephrosia purpuria root was the subject of this study. Natural phytoconstituents were all found in methanolic extracts from the root of Tephrosia purpuria.

Study Design:  Experimental research work.

Methodology: Furthermore, using UV Visible spectrophotometer equipment, the extract was scanned in the range of 380 to 900 nm, and the characteristic peaks were identified.

Results: The UV-VIS data indicated peaks at 382.70, 413.68, 536.18, 610.37, and 664.61 nm, with absorption values of 2.7930, 2.5932, 0.3114, 0.4185, and 1.5966 respectively. The presence of Natural phytoconstituents is confirmed by FTIR spectra. The findings confirm that this plant has key bioactive elements that are beneficial to our health, indicating that more research is needed.

Conclusion: Natural phytoconstituents were all found in methanolic extracts from the root of Tephrosia purpuria.

Open Access Original Research Article

Acute Kidney Injury after Ureteral Obstruction Due to Signet Ring Cell Adenocarcinoma of the Bladder

Waqas Ahmad Khalid, Naqash Siddique Gorsi, Iram Shehzadi

Journal of Pharmaceutical Research International, Page 42-45
DOI: 10.9734/jpri/2022/v34i3B35391

Background and Objectives: Acute Kidney Injury (AKI) due to bilateral ureteral obstruction in older patients typically raises concerns of abdominal, retroperitoneal, or pelvic neoplasms. Aim of the present paper is to analyse the acute kidney injury after ureteral obstruction due to signet ring cell adenocarcinoma of the bladder.

Materials and Methods: This descriptive clinical study was conducted in Lahore General Hospital, Lahore during January 2019 to November 2019. The data was collected from 10 patients of age range 40 to 60 years. This data include mainly male patients. These selected patients started with hematuria complaint, associated with left flank pain of intensity 8/10 and abrupt onset during his work.

Results: The data was collected from 10 patients of AKI after Ureteral Obstruction Due to Signet Ring Cell Adenocarcinoma of the Bladder. The mean age of the patients was 56.56±8.46 years. The BMI of the selected patients was 24.31±2.26 kg/m2 and all basic values are presented in table 01.

Conclusion: It is concluded that adenocarcinoma is a rare type of bladder cancer, with the subtype signet ring cell adenocarcinoma even rarer.

Open Access Original Research Article

Comparative Evaluation of Alpha-Amylase and Alpha- Glucosidase Inhibitory Potential of Aqueous Seed Extract of Trigonella Foenum-Graecum and Moringa Oleifera - An In vitro Study

N. Prithiksha, R. Gayathri, V. Vishnu Priya, J. Selvaraj, S. Kavitha

Journal of Pharmaceutical Research International, Page 46-54
DOI: 10.9734/jpri/2022/v34i3B35392

Background: Trigonella foenum-graecum is one of the commonly used herbs in food. Moringa oleifera is a source of food, accommodation and conventional medicine for many peoples in the developing countriesThe seeds of both the plants were explored for antidiabetic potential.

Methods: The current work was designed to probe the in vitro anti diabetic potential of the aqueous seed elicit of trigonella foenum- graceus and Moringa oleifera using the enzymes alpha amylase and alpha glucosidase.Both the extracts were screened for their phytochemicals and antioxidant potential was also analysed.The data were examine statistically by a one-way analysis of variance (ANOVA) followed by Duncan’s multiple range test was used to see the statistical significance in conjunction with groups. The results with the p<0.05 level were considered to be statistically significant.

Result: Due to its chemical ingredient and active compounds like amino acids, alkaloids,  flavonoids, it proceeds as a good antioxidant. Trigonella foenum-graecum seed extract exhibited comparatively higher antidiabetic potential with an IC50 of 300µg/ml than the Moringa oleifera extract. Results of the work designate that both extracts of the plant possessed by forbidding alpha amylase, alpha glucosidase show maximum inhibition. Hence concluded that, Trigonella foenum graecum leaves might be considered as herbal remedies for diabetes.

Conclusion: In vitro antioxidant and antidiabetic potential of seed elicit of Trigonella foenum-graecum and Moringa oleifera were analyzed and compared. This study shows that Trigonella foenum-graecum has higher antidiabetic potential than the Moringa oleifera extract.

Open Access Review Article

A Review on Antiviral Activity of Favipiravir

Mariya Palathingal, M. Archana, K. Athulya Damodharan, . Nuaman, P. Ashisha, Akash Marathakam

Journal of Pharmaceutical Research International, Page 1-7
DOI: 10.9734/jpri/2022/v34i3B35387

The emerging severe acute respiratory syndrome coronavirus 2 pandemic endangers patients and physicians all over the world. The potency of COVID-19 antiviral drugs is still a matter of debate. The growing understanding of COVID-19 virology and clinical manifestations is resulting in a larger pool of potential pharmacological targets. Favipiravir is included in the treatment regimen of many countries, including some regions of India, that have recently updated their treatment standards and is expected to be an effective treatment for SARS-CoV-2 infection, as it can be used to treat many different types of RNA virus infections such as Ebola, influenza, and the foot-and-mouth disease virus can be treated with this new nucleoside analogue. This review describes the status, mechanisms, pharmacokinetics, and antiviral effect of Favipiravir.

Open Access Review Article

Review on Different Methods of Serum Bilirubin Estimation

M. Spandana, Jayant Vagha

Journal of Pharmaceutical Research International, Page 34-41
DOI: 10.9734/jpri/2022/v34i3B35390

It is quite essential to estimate serum bilirubin in neonates with jaundice. The early prediction of jaundice is usually by observing the yellowish colour of the skin. Various methods of estimation of bilirubin have been developed and used for assessing the risk of jaundice.

Jaundice is the common neonatal problem, that with the timely diagnosis, effective treatment and proper counselling to the mother can decrease the neonatal morbidity. Proper neonatal examination, breastfeeding advice to the mother, identifying the high risk groups, proper history elicitation, quick diagnosis and early intervention can reduce the incidence of jaundice and also prevent long term complications. The main goal is to prevent the risk of severe hyperbilirubinemia and for prevention of kernicterus. Hence there is a need for a sensitive, rapid and a reliable method for the assessment of serum bilirubin. These methods include both invasive and non-invasive analysis. Invasive methods involve estimation of serum bilirubin by obtaining a plasma or serum sample and non-invasive include the assessment of bilirubin using various instruments without the requirement of blood sample from the neonate.

As the invasive method of bilirubin estimation needs more time for analysis to yield a result, many non invasive methods are being studied to find an effective tool which can give fast and accurate results ,thus making it an effective screening tool. This review article throws light on the different methods of estimating bilirubin levels in a neonate.