In vitro Antioxidant, In vivo Bioavailability, and Immunomodulatory Effects of a Polyherbal Formulation (Storg B) Induced by Cyclophosphamide in an Experimental Animal Model

Firoz H M

R and D Centre, Star Hi Herbs Pvt Ltd, Jigani, Bangalore-560105, Karnataka, India.

Sadashiva C T *

R and D Centre, Star Hi Herbs Pvt Ltd, Jigani, Bangalore-560105, Karnataka, India.

Sahana C V

R and D Centre, Star Hi Herbs Pvt Ltd, Jigani, Bangalore-560105, Karnataka, India.

*Author to whom correspondence should be addressed.


Abstract

Introduction: The immunomodulatory properties of plants are being extensively explored these days as they contain a wide range of bioactive compounds that can treat immunomodulatory disorders. The aim of the present investigation was to determine the efficacy of the standardized polyherbal formulation (Storg-B). The Storg-B combines Psidium guajava, Mangifera indica, and Citrus limon.

Methods: The aqueous extract of the Storg B was investigated for DPPH radical scavenging activity to determine antioxidant activity. SD rats were selected for the evaluation of the immunomodulatory activity of Storg-B. The haemagglutination and hematology methods were used to evaluate the immunomodulatory studies. For the pharmacokinetic studies, the LC-MS/MS technique is employed to quantify Storg B.

Results: The percentage inhibition of DPPH radial IC50 values of standard BHT and Storg-B were 60.52 and 68.35µg/ml, respectively. In the in vivo animal studies, Storg-B showed significant immunomodulatory activity by increasing the primary and secondary antibody titer values by 6.58 and 8.25 mg/mL, respectively, for the SRBC antigen at a dose of 100 mg/kg. There’s also an increase in the neutrophil adhesion percentage at the testing doses. Results of hematological parameters revealed that Storg-B restored all the levels of blood components at a 100 mg/kg dose. A Storg-B showed greater potency in the bioavailability studies.

Conclusion: Thus, the Storg-B used as an efficient and secure formulation in the treatment of immune deficiency by improving the immune response.

Keywords: Polyherbal formulation (Storg-B), antioxidant, immunomodulatory, bioavailability, B vitamins, In vitro and In vivo


How to Cite

Firoz H M, Sadashiva C T and Sahana C V (2024) “In vitro Antioxidant, In vivo Bioavailability, and Immunomodulatory Effects of a Polyherbal Formulation (Storg B) Induced by Cyclophosphamide in an Experimental Animal Model ”, Journal of Pharmaceutical Research International, 36(4), pp. 11–23. doi: 10.9734/jpri/2024/v36i47508.

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