Anti-anaemic and Anti-leukopenic Effects of Hydromethanolic Extracts of Tiger Nut and Ginger on Alloxan- induced Diabetic Rats
Samuel C. Iwuji *
Department of Human Physiology, School of Basic Medical Science, College of Medicine and Health Sciences, FUTO, Nigeria.
Kosisochukwu A. Uzokwe
Department of Biomedical Engineering, School of Engineering and Engineering Technology, Federal University of Technology Owerri (FUTO), Nigeria.
Francis O. Nwokorie
Department of Biomedical Engineering, School of Engineering and Engineering Technology, Federal University of Technology Owerri (FUTO), Nigeria.
Wilson C. Okafor
Department of Biomedical Engineering, School of Engineering and Engineering Technology, Federal University of Technology Owerri (FUTO), Nigeria.
Sixtus A. Okafor
Department of Biomedical Engineering, School of Engineering and Engineering Technology, Federal University of Technology Owerri (FUTO), Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Aims: This study investigated the anti-anaemic and anti- leukopenic effects of hydromethanolic (1:4) extracts of Cyperus esculentus (Tiger nut) and Zingiber officinale (Ginger) on the alloxan- induced diabetic albino rats.
Place: The in vivo study took place in the Department of Biochemistry, Micheal Okpara University of Agriculture Umudike, Abia state.
Methods: Fresh Cyperus esculentus and Zingiber officinale was air dried at room temperature, grounded into fine powder and extracted with hydromethanol in ratio of 20:80 using Soxhlet extraction method. The albino rats (total number of 36) were randomly divided into 6 groups of 6 rats each: Group 1 (Normal control); Group 2 (Diabetic); Group 3 (Glibenclamide); Group 4 (500mg/dl Cyperus esculentus); Group 5 (500mg/dl Zingiber officinale) and Group 6 (250mg/dl Cyperus esculentus + 250 mg/dl Zingiber officinale, 50:50) were administered orally. Group 2 to 6 were administered with 160mg/kg alloxan monohydrate intraperitoneally to induce a diabetic state. The haematological parameters determined were PCV, HB, RBC, TWBC, MCV, MCH and MCHC.
Results: The diabetic group treated with the Glibenclamide and 500 mg/kg Zingiber officinale extract had significant recovery (compared with the normal control) from the diabetic- induced depletion of Hb, PCV and RBC than the diabetic group treated with 250 mg/kg Cyperus esculentus + 250 mg/kg Zingiber officinale extracts. 500 mg/kg Cyperus esculentus extract showed the least recovery from diabetic induced anaemia.
Conclusion: Ginger extract showed value in the recovery from anaemia and leucopenia associated with diabetes having significantly improved the values of RBC, PCV, haemoglobin and TWBC in the diabetic rats more than the effects of Tigernut and combined (ginger and tigernut) extracts. Ginger extract treatment might also increase the defense mechanism of the body against infections in diabetes.
Keywords: Antidiabetic, Anti-anaemic, Anti-leucopaenic, Z. officinale, C. esculentus
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Erhabor O, Ibrahim, DG, Udomah FP, Adias T, Imoru M, Abdulrahaman Y, & Isah ZI, Kwaifa I, Hamidu AM, Onuigwe F, Ibrahim A, Hauwa B, Erhabor T. Some haematological parameters, manganese and zinc levels among diabetic patients of African descent attending Specialist Hospital Sokoto, Nigeria. Human Antibodies. 2019;27(11):1-10. DOI:10.3233/HAB-190375
Hossain P, Kawar B, Nahas ME. Obesity and diabetes in the developing world- a growing challenge. The New England Journal of Medicine. 2007; 356(3): 213-215. DOI: 10.1056/NEJMP068177
Mbata CA, Adebayo A, Chinyere N, Nyeso WA. Some Haematological Parameters in Diabetic Patients in Port Harcourt Nigeria. AJMS. 2015;3(2):2348–7186.
Biadgo B, Melku M, Abebe SM, Abebe M. Hematological indices and their correlation with fasting blood glucose level and anthropometric measurements in type 2 diabetes mellitus patients in Gondar, Northwest Ethiopia. Diabetes Metab Syndr Obes. 2016;9:91-99. DOI:10.2147/DMSO.S97563
Colak S, Geyi KF, Aslan A, Deniz GY. Effects of lichen extracts on haematological parameters of rats with experimental insulin-dependent diabetes mellitus. Toxicol Ind Health. 2014;30:878–887. DOI: 10.1177/0748233712466130
Umerie, SC, Okafor EO, Uka AS. Evaluation of the tubers and oil of Cyperus esculentus. Bioresource Technology. 1997;61(2):171-173.
Pascual B, Maroto JV, Lopez-Galarza S, Sanbautista A, Alagarda J. Chufa (Cyperus esculentus L. var. sativus Boeck.): An unconventional crop. Studies related to applications and cultivation. Economy Botany. 2000;54:439-448
Sánchez‐Zapata E, Fernández‐López J, Angel PJ. Tiger nut (Cyperus esculentus) commercialization: health aspects, composition, properties, and food applications. Comprehensive Reviews in Food Science and Food Safety. 2012;11(4):366-377.
Charles O.N, Essien E.B and Patrick-Iwuanyawu K.C. Studies on the phytochemical, proximate composition and biochemical properties of the combination of Cyperus esculentus (tiger nuts) and Phoenix dactilyfera (date fruit) flour on male albino wistar rats. Journal of Pharmacognosy and Phytochemistry. 2018;7(5): 1049-1054.
Oladele AK, Osundahunsi OF, Adebowale YA. Effect of processing techniques on the nutrients and antinutrients contents of tigernut (Cyperus esculentus l.). Nigerian Food Journal. 2009;27(2). DOI: 10.4314/nifoj.v27i2.47492
Achoribo ES, Ong MT. Tiger nut (Cyperus esculentus): Source of natural anticancer drug? Brief review of existing literature. Euromediterranean Biomedical Journal. 2017;12(19):091–094.
Sofowora EA. Medicinal plants and traditional medicinein Africa. Spectrum Books limited. 1993;159-238.
Evans W.C, Trease & Evans’ Pharmacognosy, 13th Ed. Bailliera Tindall, London. 1989:216- 217:2005
Mbaveng AT, Kuete V. Zingiber officinale. In Medicinal Spices and Vegetables from Africa Academic Press. 2017;627-639.
Nour AH, Yap SS, Nour AH. Extraction and chemical compositions of ginger (Zingiber officinale Roscoe) essential oils as cockroaches repellent. Aust J Basic Appl Sci. 2017;11(3):1–8.
Onwuka FC, Erhabor O, Eteng MU, Umoh IB. Protective effects of ginger toward cadmium-induced testes and kidney lipid peroxidation and hematological impairment in albino rats. J. Med. Food. 2011;14 (7–8):817-821.
Khaki AA, Khaki A. Antioxidant effect of ginger to prevents lead induced liver tissue apoptosis in rat. Journal of Medicinal Plant Research. 2010;4(14):1492-1495.
Otunola GA, Oloyede OB, Oladiji AT, Afolayan AJ. Comparative analysis of the chemical composition of three spices – Allium sativum L. Zingiber officinale Rosc. and Capsicum frutescens L. commonly consumed in Nigeria, African Journal of Biotechnology. 2010;9(41):6927-6931.
Adel PRS, Prakash J. Chemical composition and antioxidant properties of ginger root (Zingiber officinale), Journal of Medicinal Plants Research. 2010;4(24): 2674-2679,
Iwuji SC and Nwafor A. Phytochemical identification in the chloroform fraction of aqueous-methanol extract of Cnidoscolus aconitifolius leaves. British J. Pharmaceut. Res. 2015;5(6):437-441.
Ashok DC, Shrimant NP, Pradeep MG, Akalpita UA. Optimization of alloxan dose is essential to induce stable diabetes for prolonged period. Asian Journal of Biochemistry. 2007;2:402-408.
Frode TS, Medeiros YS. Review animal models to test drugs with potential antidiabetic. J Ethnopharmacol. 2008; 155:173-183.
Lorke D. A new approach to practical acute toxicity testing. Arch Toxicol. 1983;54: 275-287.
Khan IN, Jahan S, Bhuiya MA, Mazumder K, Saha BK. Anti-diarrheal potential of ethanol and water extracts of Euphorbia hirta whole plant on experimental animals: A comparative study scholars. Journal of Applied Medical Sciences. 2013;1(3):199-204.
Odo R, Mgbegbu EC, Anyanwu LN. Effect of aqueous ginger extract on sperm quality and haematology in lead acetate treated male albino rats. Tropical Journal of Pharmaceutical Research. 2020;19(7): 1481-1485.
Wanger H, Farnsworth NR. Economic and medical plant research: Plants and traditional medicine. Academic Press: London. 1993;4.
Walum E. Acute oral toxicity. Environ Health Perspectives. 1998;106(2):497-503.
Clark CG, Clark ML. Veterinary Toxicology. Bailirer Tindal: New York; 1977.
Ejeh SA, Onyeyili P, Abalaka SE. Anti-diarrhoea activity of the aqueous root bark extract of Byrsocarpus coccineus on castor oil-induced diarrhea in Wistar rats. Veterinary World. 2017;10(7):743-747.
Bosman D, Winkler A, Marsden J, et al. Anemia with erythropoietin deficiency occurs early in diabetic nephropathy. Diabetes Care. 2001;24:495–499.
Colak S, Geyikoğlu F, Aslan A, Deniz GY. Effects of lichen extracts on haematological parameters of rats with experimental insulin-dependent diabetes mellitus. Toxicology and Industrial Health. 2014;30(10):878-887.
Oguwike FN, Eluke BC, Eze RI, Asika CM, Nwosu PN, Nwafor C, Onumonu C. The effects of Cyperus esculentus (Tiger nut) on haematological and biochemical profile of male hypercholesteremic subjects in Uli, Anambra State Nigeria. Greener Journal of Medical Sciences. 2017; 7(4):036-041.
Airaodion AI, Ogbuagu, EO. Effect of Cyperus esculentus L.(tiger nut) milk on hepatic and renal indices of Wistar rat. Asian Journal of Research in Nephrology. 2020;3(2):10-16.
Ezekwesili NC, Ephraim O. Effects of Cyperus esculentus (tiger nut) milk on serum protein, haematological indices and antioxidant enzymes in wistar rats. Bioscientist Journal. 2016;4(1):41-45.
Kennedy L, Baynes JW. Non-enzymatic glycosylation and the chronic complications of diabetes: an overview. Diabetologia.1984;26(2):93-98.
Ganong WF. Review of Medical Physiology, 15th ed. Norwalk, Connecticut, Lange Medical Publications; 1999.
Yenigun M. Cardiovascular diabetes. Istanbul, Turkey, Istanbul University Printing House; 1997.
Olayaki LA, Ajibade KS, Gesua SS, Soladoye AO. Effect of Zingiber officinale on some hematologic values in alloxan-induced diabetic rats. Pharmaceutical Biology. 2007;45(7):556-559.
Ijioma SN, Nwankudu ON, Igwe KK. Preliminary oral acute toxicity evaluation of Euphorbia heterophylla whole plant extract. Journal of Laboratory Science. 2018;5(1): 28-32.
Saliu JA, Elekofehinti OO, Komolafe K, Oboh G. Effects of some green leafy vegetables on the haematological parameters of diabetic rats. Journal of Natural Product Plant Resources. 2012;2 (4):482-485.
Badejo AA, Damilare A, Ojuade TD. Process effects on the antioxidant activities of beverage blend development from Cyperus esculentus, Hibiscus sabdariffa and Moringa oleifera extracts. Preventive Nutrition and Food Science. 2014; 19(3):227-233.
Massadi, N.S., Ghiasvand, R., Askari, G., Hariri, M., Dawishi, L. and Mofid, M.R. (2013). Antioxidant and anti-inflammatory effects of Ginger in health and physical activity:
Madubunyi II, Onoja SO, Asuzu IU. In vitro antioxidant and in vivo antidiabetic potential of the methanol extract of Ficus glumosa Del (Moraceae) stem bark in alloxan-induced diabetic mice. Comparative clinical pathology. 2012; 21(4):389-394.
Otunola GA. Afolayan A. Antidiabetic effect of combined spices of Allium sativum, Zingiber officinale and Capsium frutescens in alloxan-induced diabetic rats. Frontiers in Life. 2014;8(4) :314-323.