Determination of the Anti-inflammatory and Anti-microbial Activity of a Cosmetic Product Silver Stop® Cream
Zhechko P. Dimitrov
LB Bulgaricum Plc, R&D Center-1113,Sofia, Bulgaria.
Teodora P. Popova
Faculty of Veterinary Medicine, University of Forestry, 10 Kl. Ohridski Blvd.-1756,Sofia, Bulgaria.
Irina M. Gotova
LB Bulgaricum Plc, R&D Center-1113,Sofia, Bulgaria.
Ignat Ignatov *
Scientific Research Center of Medical Biophysics (SRCMB), Sofia-1111, Bulgaria.
Teodora Todorova
Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, Sofia-1113, Bulgaria.
Toshka E. Petrova
Faculty of Veterinary Medicine, University of Forestry, 10 Kl. Ohridski Blvd.-1756,Sofia, Bulgaria.
Fabio Huether
EVODROP AG, 8048 Zurich, Switzerland.
*Author to whom correspondence should be addressed.
Abstract
The anti-inflammatory and antimicrobial effects of a cream containing extracts of African geranium (Pelargonium sidoides DC.), black elderberry (Sambucus nigra L.) and St. John's wort (Hypericum perforatum L.) in colloidal nanosilver (AgNPs) at a concentration of 30 ppm, denoted as SILVER STOP® cream (SS® cream) was examined In vitro. The research was performed with Staphylococcus aureus (ATCC-6538 and two clinical strains). Suspension tests for determination the time of antimicrobial action of SS® cream were used. SS® cream showed significant antimicrobial activity. In suspension with a density of 104 cells.mL-1S. aureus died after 60 minutes of exposure to SS® cream. In suspension with concentration of 106 cells.mL-1, after 2 h only single cells remained viable. The anti-inflammatory effect was evaluated using human epithelial cell line HT-29, incubated with a type strain of S. aureus. After treatment with SS® cream, results have shown a statistically significant reduction of the levels of the pro-inflammatory cytokinesIL-6, IL-8, IP-10 and IL-1β.
Keywords: Colloidal nanosilver AgNPs, Staphylococcus aureus, Pelargonium sidoides, Hypericum perforatum, Sambucus nigra, antimicrobial activity, anti-inflammatory activity
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