Determination of the Anti-inflammatory and Anti-microbial Activity of a Cosmetic Product Silver Stop® Cream

Zhechko P. Dimitrov

LB Bulgaricum Plc, R&D Center-1113,Sofia, Bulgaria.

Teodora P. Popova

Faculty of Veterinary Medicine, University of Forestry, 10 Kl. Ohridski Blvd.-1756,Sofia, Bulgaria.

Irina M. Gotova

LB Bulgaricum Plc, R&D Center-1113,Sofia, Bulgaria.

Ignat Ignatov *

Scientific Research Center of Medical Biophysics (SRCMB), Sofia-1111, Bulgaria.

Teodora Todorova

Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, Sofia-1113, Bulgaria.

Toshka E. Petrova

Faculty of Veterinary Medicine, University of Forestry, 10 Kl. Ohridski Blvd.-1756,Sofia, Bulgaria.

Fabio Huether

EVODROP AG, 8048 Zurich, Switzerland.

*Author to whom correspondence should be addressed.


The anti-inflammatory and antimicrobial effects of a cream containing extracts of African geranium (Pelargonium sidoides DC.), black elderberry (Sambucus nigra L.) and St. John's wort (Hypericum perforatum L.) in colloidal nanosilver (AgNPs) at a concentration of 30 ppm, denoted as SILVER STOP® cream (SS® cream) was examined In vitro. The research was performed with Staphylococcus aureus (ATCC-6538 and two clinical strains). Suspension tests for determination the time of antimicrobial action of SS® cream were used. SS® cream showed significant antimicrobial activity. In suspension with a density of 104 cells.mL-1S. aureus died after 60 minutes of exposure to SS® cream. In suspension with concentration of 106 cells.mL-1, after 2 h only single cells remained viable. The anti-inflammatory effect was evaluated using human epithelial cell line HT-29, incubated with a type strain of S. aureus. After treatment with SS® cream, results have shown a statistically significant reduction of the levels of the pro-inflammatory cytokinesIL-6, IL-8, IP-10 and IL-1β.

Keywords: Colloidal nanosilver AgNPs, Staphylococcus aureus, Pelargonium sidoides, Hypericum perforatum, Sambucus nigra, antimicrobial activity, anti-inflammatory activity

How to Cite

Dimitrov, Z. P., Popova, T. P., Gotova, I. M., Ignatov , I., Todorova, T., Petrova, T. E. and Huether, F. (2023) “Determination of the Anti-inflammatory and Anti-microbial Activity of a Cosmetic Product Silver Stop® Cream”, Journal of Pharmaceutical Research International, 35(4), pp. 32–43. doi: 10.9734/jpri/2023/v35i47321.


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