Frovatriptan Succinate Loaded Lipid Nanoparticles: Formulation, Evaluation, Stability Study and Shelf Life Determination
Mohd Yasir
Department of Pharmacy, College of Health Science, Arsi University, Asella, Oromia Region, Ethiopia.
Iti Chauhan
Department of Pharmaceutics, ITS College of Pharmacy, Muradnagar, Ghaziabad- (UP), India.
Madhu Verma
Hygia Institute of Pharmaceutical Education & Research, Lucknow, UP, India.
KM Noorulla
Department of Pharmacy, College of Health Science, Arsi University, Asella, Oromia Region, Ethiopia.
Abdurazak J. Tura
Department of Pharmacy, College of Health Science, Arsi University, Asella, Oromia Region, Ethiopia.
Misbahu J. Haji
Department of Pharmacy, College of Health Science, Arsi University, Asella, Oromia Region, Ethiopia.
UVS Sara
Hygia Institute of Pharmaceutical Education & Research, Lucknow, UP, India.
Murali Dadi
Department of Chemistry, School of Mathematics and Natural Sciences, Copperbelt University, Kitwe, Zambia.
Wondesen G. Gobena
Department of Pharmacy, College of Health Science, Arsi University, Asella, Oromia Region, Ethiopia.
Debesa D. Dalecha
Department of Pharmacy, College of Health Science, Arsi University, Asella, Oromia Region, Ethiopia.
Goruntla Narayana
Raghavendra Institute of Pharmaceutical Education and Research (RIPER), KR-Palli Cross, Chiyyedu, Anantapuram, Andra Pradesh, India.
Kasturi Vishwanatha Setty Veerabhadrappa *
Department of Pharmacy, College of Health Science, Arsi University, Asella, Oromia Region, Ethiopia and Raghavendra Institute of Pharmaceutical Education and Research (RIPER), KR-Palli Cross, Chiyyedu, Anantapuram, Andra Pradesh, India.
*Author to whom correspondence should be addressed.
Abstract
Aims: The aim of the research work was to prepare and optimize the Frovatriptan Succinate (FVN) loaded solid lipid nanoparticles.
Methods: SLNs were developed by solvent emulsification diffusion technique and evaluated for particle size, PDI, zeta potential, in-vitro drug release, and finally stability study was conducted for the detection of shelf life.
Results: The optimized formulation exhibited particle size, PDI, and zeta potential 122.85±9.24 nm, 0.129 and -25.85 mV, respectively. In-vitro drug release study exhibited biphasic drug release pattern. Initially (in first two hrs) the drug was release in fast manor i.e. burst release (32.36±7.28 %). It might be due to the presence of drug on the surface of SLNs. After 2 hrs of study, the release pattern became sustained up to 24 hrs. The total amount of drug release in 24 h was found to be 91.29 ± 8.26%. Various kinetic models were applied to evaluate the release pattern of the drug form the formulation. Higuchi model was found to be the best fitted with the R2 value of 0.9482. The release mechanism was found to be the Fickian type with the release exponent (n) value of 0.4386. Finally, stability study was conducted. The formulation was found to be the stable under the studied conditions. The shelf life of the formulation was found to be 1.77 years.
Conclusion: Finally, it could be concluded that, the SLNs are the suitable carrier for the delivery of FVN .
Keywords: Frovatriptan succinate, Emulsification diffusion technique, Solid lipid nanoparticles, Stability