In-Vitro Antioxidant and Pharmacognostic Studies of Phaseolus vulgaris (Linn) Seed Coat
Journal of Pharmaceutical Research International,
Page 2710-2721
DOI:
10.9734/jpri/2021/v33i60B34933
Abstract
Objectives: Pulses have grown increasingly popular as a result of their high nutritional content and phytochemical content. However, before to eating, the seed coats of some legume food items were removed, resulting in the food losing its nutritious content. The study deals with the study of pharmacognostic & physico-chemical profile along with in-vitro estimation of bioactive compounds of Phaseolus vulgaris (Linn) seed coat.
Methodology: The morphology of kidney beans was evaluated. Seed length, width, thickness, and surface area were also determined. In physico-chemical parameters extractive value ash value, moisture content, swelling index were recorded. Phytochemical screening displayed the presence of alkaloids, flavonoids, phenol, amino acid, tannins, carbohydrates and saponins. HPTLC & in-vitro estimations were done.
Results: Morphology revealed dark brownish red seed, kidney to oval shaped, medium size and bland taste. Microscopically, the transverse section showed the presence of proteinaceous aleurone cells, macro-sclereids and starch granules with irregular oval shape in the cotyledon specify the energy reservoir of seeds. HPTLC showed the presence of flavonoids in Phaseolus vulgaris seed coats. The antioxidant profile revealed TFC (total flavonoid content) as 13.62 mg/g QE (quercetin equivalent) and TPC (total phenolic content) as 32.03 ± 1.50 mg/g GAE. IC50 value for vitamin C was found to be 369.03 µg/ml as compared to Phaseolus vulgaris seed coat 423.00µg/ml.
Conclusion: The study can serve as a valuable source of information and due to presence of phytoconstituents like flavonoid (quercetin) it could be considered for its neuroprotective activity.
Keywords:
- Red kidney bean
- flavonoids
- antioxidant
- morphology
- seed coat
How to Cite
References
Sasanam S, Paseephol T and Moongngarm A. Comparison of proximate compositions, resistant starch content, and pasting properties of different colored cowpeas (Vigna unguiculata) and red kidney bean (Phaseolus vulgaris). World Acad. Sci. Eng. Technol. 2011;5(9):553-557.
Moses O, Olawuni I, Iwouno JO. The proximate composition and functional properties of full-fat flour, and protein isolate of lima bean (Phaseolus lunatus). Sci. Rep. 2012;1(7):1-5.
Mudryj AN, Yu N, Hartman, TJ, Mitchell DC, Lawrence FR and Aukema HM. Pulse consumption in Canadian adults influences nutrient intakes. Br. J. Nutr. 2012;108(S1):S27-36.
Wang N, Hatcher DW, Tyler RT, Toews R, Gawalko EJ. Effect of cooking on the composition of beans (Phaseolus vulgaris L.) and chickpeas (Cicer arietinum L.). Food Res. Int. 2010;43(2):589- 594.
Copmans D, Steurs G. Methylated flavonoids as anti-seizure agents: Naringenin 4, 7-dimethyl ether attenuates epileptic seizures in zebrafish and mouse models, Neurochem Int. 2018;112:124-133.
Sefil F, Ibrahim Kahraman, Neslihan Pinar. Ameliorating effect of Quercetin on acute PTZ induced seizures in Rats. International Journal of Clinical and experimental medicine. 2014;7(9):2471-2477.
Kola PK, Akula A, Nissankara Rao LS, Danduga RSR. Protective effect of naringin on pentylenetetrazole (PTZ) induced kindling, Epilepsy Behav. 2017;75:114-126.
Nassiri M, Reza Martazavi, Fatemeh Samiee-Rad, Esmail Abbasi. The effect of rutin on the development of PTZ kindling and memory retrieval in rats. Epilepsy behave; 2010.
Akash, Sumitra S, Shailendra KS. Antioxidant and anti-inflammatory potential of kidney bean (Phaseolus vulgaris L). International research journal of pharmacy; 2018.
Luka CD, Olatunda A, Tijjani H, Olisa-Enewe IA. Effect of aqueous extract of Phaseolus vulgaris (red kidney bean) on alloxan induced diabetic wistar rats. International Journal of Science Invention Today. 2013;2(9):292-301.
Ombra MN, d’Acierno A, Nazzaro F, Riccardi R, Spigno P, Zaccardelli M, Fratianni F. Phenolic composition and antioxidant and antiproliferative activities of the extracts of twelve common bean (Phaseolus vulgaris L.) endemic ecotypes of Southern Italy before and after cooking. Oxidative Medicine and Cellular Longevity. 2016;13(9):82-98.
Rashmi A, Bhakti S, Yash N, Tejas G. Evaluation and comparision of Anti Parkinson activity of methanolic extract of Phaseolus vulgaris with L-DOPA. International journal of Pharmacology Research. 2015;103-108.
Porcher MH. Phaseolus vulgaris L. In: Multilingual multiscript plant name database; 2013. Available:http://www.plantnames.unimelb.edu.au/Sorting/Phaseolus.html [Cited 2016 14th Oct].
USDA. 2005. National Nutrient Database for Standard Reference Release 27: Basic Report 16032, Beans, Kidney, red, mature seeds, raw. [Cited 2016 Sept 23, 03:45EDT]; Available:http://ndb.nal.usda.gov/ndb/nutrients/index Accessed 23 September 2016.
Lin LZ, Harnly JM, Pastor-Corrales MS, Luthria DL. The polyphenolic profiles of common bean (Phaseolus vulgaris L.). Food Chemistry. 2008;107(1):399– 410.
Borkar A, More AD. Induced flower colour mutations in Phaseolus vulgaris Linn through physical and chemical mutagens. Adv Biores. 2010;1(1):22-8.
Oomah BD, Corbe A and Balasubramanian P. Antioxidant and anti-inflammatory activities of bean (Phaseolus vulgaris L.) hulls. Journal of Agricultural and Food Chemistry. 2010;58(14):8225-8230.
Reynoso C, Ramos G, Loarca P. Bioactive components in common beans (Phaseolus vulgaris L.): Advances in Agricultural and Food Biotechnology. In: GuevaraGonzález RG, Torres-Pacheco I, ed. Research Signpost. Trivandrum, Kerala, India. 2006;217-236.
Marshall JJ, Lauda CM. Purification and properties of phaseolamin, an inhibitor of alpha-amylase from the kidney bean Phaseolus vulgaris. Journal of Biological Chemistry. 1975;250(20):8030- 8037.
Rana JH, Patel UR, Patel SJ, Lambole V, Shah, DP. Pharmacological activities of Phaseolus vulgaris: A review. Pharma Science Monitor. 2016;7(2):107- 115.
Madhu S, Ramica S. Antidepressant activity of aqueous extract of Phaseolus vulgaris in rodent models of depression. International Journal of Nutrition, Pharmacology, Neurological Diseases. 2014;4:118-124.
Mohsenin NN. Physical properties of plant animal material II. NEWYORK USA: Gordon and Breach Sci. 1986;841- 881.
Jain RK, Bal D. Properties of pearl millet. J Agric Engg Res. 66 (2):85-91.
Kokate CK, Purohit AR, Gokhale SB. Pathway to Screen Phytochemical nature of Natural Drugs. Pharmacognosy, 42nd ed, Nirali Prakashan, Pune. 2008;56- 61.
Harborne JB. Phytochemical methods, A guide to modern techniques of plant analysis. 3rd ed, Springer Pvt. Ltd. New Delhi, India. 1998;40-107.
Kim D, Jeong S, Lee C. Antioxidant capacity of phenolic phytochemicals from various cultivars of plums. Food Chemistry. 2003;81(6):321-326.
Slinkard K, Singleton VL. Total phenol analysis: Automation and comparison with manual methods. Am J Enol Vitic. 1977;28:49-55.
Lillian B, Telma C, Paula B, Leticia ME, Isabel F. Wild and commercial mushrooms as source of nutrients and nutraceuticals. Food and Chemical Toxicology. 2008;46:2742-2747.
Baldivia DDS, Leite DF, De Castro DTH, Campos JF, Dos Santos UP, Paredes-Gamero EJ, Carollo CA, Silva DB, De Picoli SK, Dos Santos EL. Evaluation of in vitro antioxidant and anticancer properties of aqueous extract from stem bark of Stryphnodendron adstringens. Int J Mol Sci. 2018;19:2432.
Zainish MS, Salman A, Muhammad MH. Phaseolus vulgaris L.: Botany, medicinal uses, phytochemistry and pharmacology. World Journal of Pharmaceutical Research. 2016;5:1611-1615.
Waziri AN, Mittal JP. Design–related physical properties of selected agricultural products. Food and Agriculture Organization of the United Nations. 2013;14:59-62.
Joseph SG, Barry GS. Microstructure of black, green and red gram. Food Structure. 1993;12(2):155-162.
Peerzada AM, Ali HH, Naeem M, Latif M, Bukhari AH, Tanveer A. Cyperus rotundus L.: Traditional uses, phytochemistry, and pharmacological activities. J Ethnopharmacol. 2015;174:540-560.
Akhtar S, Ismail T, Fraternale D, Sestili P. Pomegranate peel and peel extracts: Chemistry and food features. Food Chem. 2015;174:417-425.
Mishra J, Singh RD, Jadon VS, Gusain MP, Aradhana. HPTLC profile of Quercetin content of common bean. American Journal of Food Technology. 2012;7(2):96-100.
Khorasani EA, Mat TR, Mohajer S, Banisalam B. Antioxidant activity of total phenolic and flavonoid content of various solvent extracts from in vivo and in vitro grown Trifolium pratense L. (Red Clover). BioMed Res Int. 2015;643285.
Nambooppha B, Photichai K, Wongsawan K, Chaummitri P. quercetin manipulates the expression of genes involved in the reactive oxygen species (ROS) process in chicken heterophils. J Vet Med Sci. 2018;80:1204-1211.
-
Abstract View: 165 times
PDF Download: 50 times
