Evaluation of Anti-inflammatory and Antioxidant Effects of Punicalagin (Pomegranate Extract) on IL-1 Beta and Superoxide Dismutase Levels in Patients with Chronic Periodontitis – A Randomized Controlled Trial
Journal of Pharmaceutical Research International,
Page 718-726
DOI:
10.9734/jpri/2021/v33i60A34538
Abstract
Purpose: Pomegranates have been shown to possess anti-inflammatory and antioxidant properties. This study aimed to evaluate the effects of Punicalagin (Pomegranate extract) on IL-1β and SOD levels in chronic periodontitis patients.
Materials and Methods: 30 patients were included in the study. The sites were randomly divided into control site and test site. Control sites were treated with Scaling and Root Planing (SRP) alone and test sites were treated with punicalagin gel along with SRP. Clinical parameters such as probing pocket depth, Clinical Attachment Level (CAL), Gingival Index (GI), Plaque index (PI), Sulcus Bleeding Index (SBI) and biochemical markers such as IL-1β and Superoxide Dismutase levels were compared between baseline and at 21 days post therapy.
Results: Test sites showed significant differences when compared to control sites in relation to all clinical and biochemical parameters.
Conclusion: Within the limitations of this study, there is an improvement in the clinical parameters with an increase in the anti-oxidant marker level and a decrease in inflammatory marker level when punicalagin gel was placed along with Scaling and Root Planing in patients with Chronic Periodontitis.
Keywords:
- Punicalagin
- IL-1β
- superoxide dismutase
- chronic periodontitis
How to Cite
References
Bhardwaj A, Mahajan A, Thakur N, Kumar N. Mechanical non surgical therapy: an indispensable tool. IOSR Journal of Dental and Medical Sciences (JDMS). 2012;1: 36-41.
Walker CB. The acquisition of antibiotic resistance in the periodontal microflora. Periodontol. 2000;1996:10:78-88.
Singh G, Navkiran, Kaur S, Singh ST. Local drug delivery in periodontics: a review. J Periodontal Med Clin Pract. 2014; 01: 272-284.
Kala SB, Gunjan C, Disha N and Shobha P. Treatment of periodontal disease – a herbal approach. Int. J. Pharm. Sci. Rev. Res. 2015;33(2):126-136.
Page RC. The role of inflammatory mediators in the pathogenesis of periodontal disease. J Periodont Res. 1991;26: 230-242.
Karim S, Pratibha PK, Kamath S, Bhat SG, Kamath U, Dutta B, Sharma N, Archana B, Bhat MK, Guddattu V. Superoxide dismutase enzyme and thiol antioxidants in gingival crevicular fluid and saliva. Dental Research Journal. 2012;9(3):266-272.
Malik A, Afaq F, Sarfaraz S, Adhami MV, Syed DN, Mukhtar H. Pomegranate fruit juice for chemoprevention and chemotherapy of prostate cancer. PNAS. 2005; 02(41):14813–14818.
Naz S, Siddiqi R, Ahmad S, Rasool SA, and Sayeed SA. Antibacterial activity directed isolation of compounds from Punica granatum. Journal of Food Science. 2007;72(9):341-345.
Jurenka JMT. Therapeutic applications of pomegranate (Punica granatum L.): A review. Alternative Medicine Review. 2008; 13(2):128-144.
Bosshardt DD and Sculean A. Does Periodontal Tissue Regeneration Really Work? Periodontology. 2000;2009;(51): 208–219.
Xu X, Li H, Hou X, Li D, He S, Wan C, Yin P, Liu M, Liu F, Xu J. Punicalagin Induces Nrf2/HO-1 Expression via Upregulation of PI3K/AKT Pathway and Inhibits LPS-Induced Oxidative Stress in RAW264.7 Macrophages. Mediators of Inflammation. 2015;1-11.
Bhadbhade SJ, Acharya AB, Rodrigues SV and Thakur SL. The antiplaque efficacy of pomegranate mouthrinse. Quintessence Int. 2011;42:29–36.
Sahgal A, Chaturvedi SS, Bagde H, Agrawal P, Suruna R, Limaye M. A randomized control trial to evaluate efficacy of anti-bacterial and anti-inflammatory effect of aloe vera, pomegranate and chlorhexidine gel against periodontopathogens. Journal of International Oral Health. 2015;7(11):33-36.
Ahuja S, Dodwad V, Kukreja BJ, Mehra P and Kukreja P. A comparative evaluation of efficacy of Punica granatum and chlorhexidine on plaque and gingivitis. J Int Clin Dent Res Organ. 2011;3:29– 32.
Sastravaha et al. Adjunctive periodontal treatment with Centella asiatica and Punica granatum extracts in supportive periodontal therapy. Journal of the International Academy of Periodontology 2005;7(1):1-10.
Xu X, Yin P, Wan C, Chong X, Liu M, Cheng P, Chen J, Liu F, Xu J. Punicalagin inhibits inflammation in LPS - Induced Raw264.7 macrophages via the suppression of Tlr4-Mediated MAPKS And NF-Κβ activation. Inflammation 2014; 37(3):956-965.
Ahmed S, Wang N, Hafeez BB, Cheruvu VK, Haqqi TM. Punica granatum L. Extract Inhibits IL-1β–Induced Expression of Matrix Metalloproteinases by Inhibiting the Activation of MAP Kinases and NF-κB in human chondrocytes in vitro. J Nutr. 2005; 135(9):2096–2102.
Xu J, Zhao Y, Aisa HA. Anti-inflammatory effect of pomegranate flower in lipopolysaccharide (LPS)-Stimulated RAW264.7 macrophages. Pharmaceutical Biology. 2017;55(1):2095–2101.
Gil M, Barbera FAT, Pierce BH, Holcroft DM, Kader AA. Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing. J. Agric Food Chem. 2000;48: 4581- 4589.
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