Collagen-Chitosan Composite Powder as a Novel Biomaterial for Chronic Wounds in Hansen Disease-A Clinical Study
Journal of Pharmaceutical Research International,
Page 154-165
DOI:
10.9734/jpri/2021/v33i49B33352
Abstract
Aims: Natural biomaterials are more suitable than synthetic biomaterials for in vivo applications for treating damaged tissues. Collagen and chitosan are abundantly available natural biomaterials for wound dressings for tissue/wound repair. In this context, collagen-chitosan composite powder has been used to treat chronic wounds in Hansen disease (HD) patients.
Place and Duration of Study: CSIR Central Leather Research Institute, Sardar Patel Road, Adyar, Chennai 600021, Southern Railway Headquarters Hospital, Constable Road, Ayanavaram, Chennai 600023, and Gremaltes Hospital, India between June 2013 and July 2020.
Methodology: Collagen extracted from bovine rumen, a waste product of meat industry, and a commercially available chitosan were prepared as a composite powder (COL/CS) and applied to chronic wounds in HD patients after debridement and the wound contours were measured by planimetry. Biochemical parameters in blood samples were periodically assessed. Histopathology of wound tissue with Hematoxylin and Eosin and Masson’s Trichrome staining was studied. Matrix Metalloproteinase-9 (MMP9) levels before and after treatment were estimated.
Results: Wound healing of 64.2% was obtained with COL/CS treatment and formation of granulation cells was observed early. Hemogram studies have been reported in a regression model with 95% confidence intervals. Histopathology revealed dense collagen fibres and continuity of sub-epithelial layer on 8th day. MMP-9 levels showed collagen integrity after treatment.
Conclusion: The novel biocompatible, biodegradable COL/CS wound dressing is a promising biomaterial for management of chronic wounds in Hansen disease patients.
Keywords:
- Chitosan
- collagen
- chronic wounds
- hansen disease
- wound dressing
How to Cite
References
Zhang MX, Zhao WY, Fang QQ, Wang XF, Chen CY, Shi BH, Zheng B, Wang SJ, Tan WQ, Wu, LH. Effects of chitosan-collagen dressing on wound healing in vitro and in vivo assays. Journal of Applied Biomaterials and Functional Materials. 2021;19:1-10.
Rajam AM, Mala JGS, Rose C, Mandal AB. Chitosan-based nanoparticles as growth factor delivery systems for tissue engineering applications. In: Govil JN, editor, Nanotechnology Vol.11, Studium Press LLC, USA; 2014.
Dong C, Lv Y. Application of collagen scaffold in tissue engineering: Recent advances and new perspectives. Polymers. 2016;8:42.
Shankar KG, Udhayakumar S, Sowndarya S, Suresh Babu P, Rose C. Isolation, characterization and in vitro evaluation of bovine rumen submucosa films of collagen or chitosan-treated collagen. Journal of Biomaterials Applications. 2016;30:780-792.
Amaral MB, Viana RB, Viana KB, Diagone CA, Denis AB, Plepis AMG. In vitro and in vivo response of composites based on chitosan, hydroxyapatite and collagen. Acta Scientarium Technology. 2020;42: e41102.
Vojtova L, Pavlinakova, V., Muchova, J. Kacvinska, K, Brtnikova J, Knoz M. et al. Healing and angiogenic properties of collagen/chitosan scaffolds enriched with hyperstable FGF2- STAB® protein: in vitro, ex ovo and in vivo comprehensive evaluation. Biomedicines. 2021;9(6):590.
Feng P, Luo Y, Ke C, Qiu H, Wang W, Zhu Y, Hou R, Xu L, Wu S. Chitosan-based functional materials for skin would repair: Mechanisms and Applications. Frontiers in Bioengineering and Biotechnology. 2021; 9:1-15.
Andritoiu CV, Andriescu CE, Danu M, Lungu C, Ivanescu B, Havarneanu C, Popa M. Evaluation of the wound healing potential of some natural polymers on three experimental models. Pharmaceuticals. 2021;14:465.
Xu R, Luo G, Xia H, He W, Zhao J, Liu B et al. Novel bilayer wound dressing composed of silicone rubber with particular micropores enhanced wound re-epithelialization and contraction. Biomaterials. 2015;40:1-11.
Zhao X, Wu H, Guo B, Dong R, Qiu Y, Ma PX. Antibacterial, antioxidant, electroactive injectable hydrogel as self-healing wound dressing with hemostasis and adhesiveness for cutaneous wound healing. Biomaterials. 2017;122:34-47.
Falanga V. Wound healing and its impairment in the diabetic foot. Lancet 2005;366:1736-1743.
Leng QQ, Li Y, Pang XL, Wang BQ, Wu ZX, Lu Y, Xiong K, Zhao L, Zhou P, Fu SZ Curcumin nanoparticles incorporated in PVA/collagen composite films promote wound healing. Drug delivery. 2020;27(1): 1676-1685.
Guo S, DiPietro LA. Factors affecting wound healing. Journal of Dental Research. 2010;89(3):219-229.
Mohammadi Z, Zak MS, Majdi H, Mostafavi E, Barati M, Lotfimehr H et al. The effect of chrysin-curcumin-loaded nanofibers on the wound healing process in male rats. Artificial Cells, Nanomedicine and Biotechnology. 2019;47:1642-1652.
Santhanam R, Rameli MAP, Jeffri AA, Ismail WIW. Bovine-based collagen dressings in wound care management. Journal of Pharmaceutical Research International. 2020;32(33):48-63.
Udhayakumar S, Shankar KG, Sowndarya S, Rose C Novel fibrous collagen-based cream accelerates fibroblasts growth for wound healing applications: in vitro and in vivo evaluation. Biomaterials Science. 2017;5:1868-1883.
Das A, Abas M, Biswas N, Banerjee P, Ghosh N, Rawat A et al. A modified collagen dressing induces transition of inflammatory to reparative phenotype of wound macrophages. Scientific Reports. 2019;9:14293.
Ghomi ER, Khalili S, Khorasani SN, Neisiany RE, Ramakrishna S. Wound dressings: Current Advances and Future directions. Journal of Applied Polymer Science. 2019;47738.
Kong Y, Tang X, Zhao Y, Chen X, Yao K, Zhang L et al. Degradable tough chitosan dressing for skin wound recovery. Nanotechnology Reviews 2020;9:1576-1585.
Upadya GM, Govindarajan S. Novel treatment for Hard-to-heal chronic plantar ulcers in Hansen’s disease. Journal of Clinical and Diagnostic Research. 2019; 13(3):WD01-WD03.
Miyashiro D, Cardona C, Valente NYS, Avancini J, Benard G, Trindade MAB. Ulcers in leprosy patients, an unrecognized clinical manifestation: a report of 8 cases. BMC Infectious diseases. 2019;19:1013.
Upputuri B, Srikantam A, Mamidi RS. Comorbidities associated with non-healing associated with plantar ulcers in leprosy patients. PLOS Neglected Tropical Diseases. 2020;14(6):e0008393.
Kim CL, Kim DE. Self-healing characteristics of collagen coatings with respect to surface abrasion. Scientific Reports 2016;6:20563.
Tamer TM, Valachova K, Hassan MA, Omer AM, El-Shafeey M, Eldin MSM et al. Chitosan/ hyaluronan/ edaravone membranes for anti-inflammatory wound dressing: in vitro and in vivo evaluation studies. Materials Science and Engineering: C: Materials for Biological Applications. 2018;90:227-235.
Jayakumar R, Prabaharan M, Sudheeshkumar PT, Nair SV, Tamura H. Biomaterials based on chitin an chitosan in wound dressing applications. Biotechnology Advances. 2011;29:322-337.
Vijayan A, Nanditha CK, Kumar GSV. ECM-mimicking nanofibrous scaffold enriched with dual growth factor carrying nanoparticles for diabetic wound healing. Nanoscale Advances. 2021;3:3085-3092.
Rohani MG, Parks WC. Matrix remodeling by MMPs during wound repair. Matrix Biology. 2015;44-46:113-121.
Caley MP, Martins VLC, O’Toole EA. Metalloproteinases and wound healing. Advances in Wound Care. 2015;4(4):225-233.
Rayment EA, Upton Z, Shooter GK. Increased matrix metalloproteinase-9 (MMP9) activity observed in chronic wound fluid is related to the clinical severity of the ulcer. British Journal of Dermatology. 2008;158:951-961.
-
Abstract View: 249 times
PDF Download: 68 times
