Role of Immunohistochemistry Versus he Stain in Helicobacter pylori Detection in Gastric Lesions
Journal of Pharmaceutical Research International,
Page 21-35
DOI:
10.9734/jpri/2021/v33i21A31365
Abstract
Helicobacter pylori are a spiral campylobacter like bacteria which infects the stomach causing chronic active gastritis. This can result in peptic ulcer disease, gastric adenocarcinoma, and mucosa-associated lymphoid tissue lymphoma. In 1989, studies involving 16S ribosomal RNA gene sequencing and others revealed that the bacterium does not come under the genus Campylobacter. Hence the bacterium was classified under a unique genus named Helicobacter. This term is taken from the Greek language with helix meaning - "spiral" or "coil" and pylori meaning- gatekeeper (pylorus of stomach). This bacterium is said to penetrate the pylori or mucoid lining of the stomach. When infected during the early stages of life, people develop intense inflammation that may be followed by atrophic gastritis which serves as a risk factor for gastric ulcer, gastric cancer or both. Being infected during later stages of life changes the gastric system leading to duodenal ulcer. The present study analyzes the role of Immunohistochemistry versus Hematoxylin and Eosin and special stains in detecting Helicobacter pylori in gastric lesions.
Keywords:
- Helicobacter pylori
- peptic ulcer disease
- gatekeeper
- gastritis
How to Cite
Narain, A., Karkuzhali, P., Kumar, R., & Thanka, J. (2021). Role of Immunohistochemistry Versus he Stain in Helicobacter pylori Detection in Gastric Lesions. Journal of Pharmaceutical Research International, 33(21A), 21-35. https://doi.org/10.9734/jpri/2021/v33i21A31365
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Wabinga HR. Comparison of immunohistochemical and modified giemsa stains for demonstration of Helicobacter pylori infection in an African population. Afr Health Sci. 2002;2(2):52 –55.
Tulika Singh, Sanju Sharma. Seetharamiah Nagesh Socio-economic status scales updated for 2017 International Journal of Research in Medical Sciences. 2017;5(7):3264- 3267.
Pandey Arvind , Tripathi Satyendra Chandra, Mahata Sutapa, Vishnoi Kanchan, Shukla Shirish, Sri Prakash Misra, Misra Vatsala, Hedau Suresh, Mehrotra Ravi, Dwivedi Manisha , Alok C Bharti. Carcinogenic Helicobacter pylori in gastric pre-cancer and cancer lesions: Association with tobacco- chewing. World J Gastroenterol. 201414;20(22):6860–6868.
Khokar N, Gill ML. Gastrointestinal injury from non steroidal anti-inflammatory drugs. Rawal Med J. 2003;28(2):22-4.
Anderson J, Anderson JV, Baker LRI, Benjamin N, Black GM, Borroughs AK, et al. Gastrointestinal disease. In: Kumar and Clark Clinical medicine: 5th ed. Philadelphia: WB Saunders. 2002; 272.
Minhas KZ, Goraya F, Javid S, Shakoor A, Rizwan A, Hashmi MT . Endoscopic and Histopathological evaluation of 306 dyspepsic patients. Pak J Gastroentrol. 2003;17:4 -7.
Genta R, Graham D. Comparison of biopsy sites for the histopathologic diagnosis of Helicobacter pylori: A topographic study H.pylori density and distribution. Gastrointest Endosc 1994;40:342.
Fox JG, Wang TC: Inflammation, atrophy and gastric cancer .J Clin Invest. 2007;117:60 -9.
Liddell HG and Scott R. A Lexicon: Abridged from Liddell and Scott’s Greek – English Lexicon . Oxford [Oxfordshire]: Oxford University Press; 1996.
ISBN: 0- 19-910207-4.
Brown LM. “Helicobacter pylori: epidemiology and routes of transmission”. Epidemiol Rev 2000;22: 283-97.
Pounder RE, Ng D. "The prevalence of Helicobacter pylori infection in different countries". Aliment.Pharmacol.Ther. 1995;9(Suppl 2):33–9.
Kusters JG, van Vliet AH, Kuipers EJ. "Pathogenesis of Helicobacter pylori Infection". Clin Microbiol Rev. 2006;19(3):449–90.
Bancroft JD, Gamble M. Theory and practice of histological techniques. 6th ed, Elsevier, Philadelphia; 2008.
Childs GV, Unabia G. Application of a rapid avidin-biotin-peroxidase complex (ABC) technique to the localization of pituitary hormones at the electron microscope level. J Histochem Cytochem. 1982;30(12):1320-1324.
Kumar Rajesh, Bano G, Kapoor B, Sharma Sunil, Gupta Yudhvir. Clinical Profile in H. Pylori Positive Patients in Jammu. JK Science. 2006;3:148 -50.
Babić T, Basić H, Miljković B, Kocić B, Tasić G.Detection of Helicobacter pylori in gastric biopsy and resection specimens. Vojnosanit Pregl. 2005;62(1):39-43.
Dixon MF, Genta RM, Yardley JH, et al. Classification and grading of gastritis. The updated sydney system. International workshop on the histopathology of gastritis, Houston 1994. Am J Surg Pathol. 1996;20:1161-81
Hartman DJ, Owens SR. Are routine ancillary stains required to diagnose Helicobacter infection in gastric biopsy specimens? An institutional quality assurance review. Am J Clin Pathol 2012;137:255 -60.
Robbins SL, Cotran RS, Kumar V,Abbas AK,Fausto N, Aster JC. Robbins and Cotrans pathologic basis of diseases, 8th Editio.Elsevier Saunders, Philadelphia, Pa; 2009.
Ko JK, Cho CH. Alcohol drinking and cigarette smoking: a "partner" for gastric ulceration. Zhonghua Yi Xue Za Zhi. 2000;63:845 -54.
Yangchun Zhu, Xiaoying Zhou, Junbei Wu, Jing Su2 and Guoxin Zhang. Risk factors and prevalence of Helicobacter pylori infection in persistent high incidence area of gastric Carcinoma in Yangzhong City. Gastroenterology Research and Practice Volume. 2014;10 Article ID: 481365,
Shokrzadeh L, Baghaei K, Yamaoka Y, Shiota S, Mirsattari D, Porhoseingholi A, et al. Prevalence of Helicobacter pylori infection in dyspeptic patients in Iran. Gastroenterol Insights. 2012;4:24–7.
Shokrzadeh L, Baghaei K, Yamaoka Y, Shiota S, Mirsattari D, Porhoseingholi A, et al. Prevalence of Helicobacter pylori infection in dyspeptic patients in Iran. Gastroenterol Insights. 2012;4:24–7.
Adisa JO1, Musa AB2, Yima UI2, Egbujo EC3. Helicobacter Pylori associated gastritis In North- Eastern Nigeria: A Histopathologic Study. 2011;3:1749- 53.
Javed M, Amin K, Muhammad D, Husain A, Mahmood N. Prevalence of H. Pylori. Professional Med Sep. 2010;17(3):431-439.
Kaore NM, Nagdeo NV, Thombare VR. Comparative evaluation of the diagnostic tests for Helicobacter pylori and dietary influence for its acquisition in dyspeptic patients: A rural hospital based study in central India. JCDR. 2012;6:636–41.
Adlekha S, Chadha T, Krishnan P, Sumangala B . Prevalence of Helicobacter pylori infection among patients undergoing upper gastrointestinal endoscopy in a medical college hospital in Kerala, India. Ann Med Health Sci Res. 2013;3(4):559–563.
Wabinga HR. Comparison of immunohistochemical and modified giemsa stains for demonstration of Helicobacter pylori infection in an African population. Afr Health Sci. 2002;2(2):52 –55.
Tulika Singh, Sanju Sharma. Seetharamiah Nagesh Socio-economic status scales updated for 2017 International Journal of Research in Medical Sciences. 2017;5(7):3264- 3267.
Pandey Arvind , Tripathi Satyendra Chandra, Mahata Sutapa, Vishnoi Kanchan, Shukla Shirish, Sri Prakash Misra, Misra Vatsala, Hedau Suresh, Mehrotra Ravi, Dwivedi Manisha , Alok C Bharti. Carcinogenic Helicobacter pylori in gastric pre-cancer and cancer lesions: Association with tobacco- chewing. World J Gastroenterol. 201414;20(22):6860–6868.
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