Adherence of Streptococcus mutans to Nanoceramic and Nanohybrid Resin Composites: An In vitro Study
Journal of Pharmaceutical Research International,
Page 128-133
DOI:
10.9734/jpri/2020/v32i2530831
Abstract
Streptococcus mutans (S. mutans) is one of the cariogenic microorganisms. The restorative materials which harbor a biofilm with high number of S. mutans can accelerate the occurrence of dental caries. The purpose of this study was to evaluate the adherence of S. mutans to nanoceramic and nanohybrid resin composites. Fifteen discs of each material (Nanohybrid resin composite, Nanoceramic resin composite) were prepared, polished, and sterilized in a gamma radiation chamber. Specimens were exposed to the S. mutans bacterial suspension (0.5 McFarland) and were incubated for 4 hours. Specimens were rinsed and sonicated in normal saline, 10 μl of the obtained suspension was cultured in a sterile blood agar medium. After 24 hours, the number of colony forming units of S. mutans was counted. A sterility test control was considered for each group of materials. The data was analyzed by Independent t test. The means and standard deviations of the logarithmic counts of the colonies on the surfaces of nanohybrid resin composites and nanoceramic resin composite were equal to 3.2±0.87 and 2.8±0.324 respectively. Ceram X Universal did not show any significant difference in the bacterial adhesion compared to Filtek Z350XT. Both composites showed similar behaviour in terms of S. mutans colonization in a simple biofilm formation model.
Keywords:
- Bacterial adhesion
- Ceram X universal
- dental restoration
- Filtek Z350XT
- Streptococcus mutans.
How to Cite
Jaju, K., & Nasim, I. (2020). Adherence of Streptococcus mutans to Nanoceramic and Nanohybrid Resin Composites: An In vitro Study. Journal of Pharmaceutical Research International, 32(25), 128-133. https://doi.org/10.9734/jpri/2020/v32i2530831
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Hansel C, Leyhausen G, Mai UEH, et al. Effects of various resin composite (Co) monomers and extracts on two caries-associated micro-organisms in vitro. Journal of Dental Research. 1998;77:60-67.
Xu S, Guo J, Huang J, et al. Effect of two dental restorative materials on adhesion and molecular structure of oral bacteria. Journal of Nanoscience and Nanotechnology. 2020;20:4643–4647.
Adhesion of Streptococcus mutans to zirconia, titanium alloy and some other restorative materials: ‘An in-vitro study’. Advances in Bioscience and Clinical Medicine; 4. Epub ahead of print; 2015. DOI: 10.7575/aiac.abcmed.15.03.02.04
Basta AH, El-Saied H, Deffallah EM. Optimising the process for production of high performance bagasse-based composites from rice bran-UF adhesive system. Pigment & Resin Technology. 2014;43:212–218.
Marghalani HY. Effect of filler particles on surface roughness of experimental composite series. J Appl Oral Sci. 2010; 18:59–67.
Tuncer S, Demirci M, Tiryaki M, et al. The effect of a modeling resin and thermocycling on the surface hardness, roughness, and color of different resin composites. Journal of Esthetic and Restorative Dentistry. 2013;25:404–419.
Courtney HS, Hasty DL. Aggregation of group A streptococci by human saliva and effect of saliva on streptococcal adherence to host cells. Infection and Immunity. 1991;59:1661–1666.
Ramarao S, Sathyanarayanan U. CRA Grid - A preliminary development and calibration of a paper-based objectivization of caries risk assessment in undergraduate dental education. J Conserv Dent. 2019; 22:185–190.
Poorni S, Srinivasan MR, Nivedhitha MS. Probiotic strains in caries prevention: A systematic review. J Conserv Dent. 2019; 22:123–128.
Manohar MP, Sharma S. A survey of the knowledge, attitude, and awareness about the principal choice of intracanal medicaments among the general dental practitioners and nonendodontic specialists. Indian J Dent Res. 2018;29: 716–720.
Azeem RA, Sureshbabu NM. Clinical performance of direct versus indirect composite restorations in posterior teeth: A systematic review. J Conserv Dent. 2018; 21:2–9.
Jenarthanan S, Subbarao C. Comparative evaluation of the efficacy of diclofenac sodium administered using different delivery routes in the management of endodontic pain: A randomized controlled clinical trial. J Conserv Dent. 2018;21:297–301.
Nandakumar M, Nasim I. Comparative evaluation of grape seed and cranberry extracts in preventing enamel erosion: An optical emission spectrometric analysis. J Conserv Dent. 2018;21:516–520.
Malli Sureshbabu N, Selvarasu K, V JK, et al. Concentrated growth factors as an ingenious biomaterial in regeneration of bony defects after periapical surgery: A report of two cases. Case Rep Dent. 2019;2019:7046203.
Siddique R, Nivedhitha MS, Jacob B. Quantitative analysis for detection of toxic elements in various irrigants, their combination (precipitate), and para-chloroaniline: An inductively coupled plasma mass spectrometry study. J Conserv Dent. 2019;22:344–350.
Teja KV, Ramesh S, Priya V. Regulation of matrix metalloproteinase-3 gene expression in inflammation: A molecular study. J Conserv Dent. 2018;21:592–596.
Rajakeerthi R, Ms N. Natural Product as the Storage medium for an avulsed tooth – A systematic review. Cumhuriyet Dental Journal. 2019;22:249–256.
Siddique R, Nivedhitha MS. Effectiveness of rotary and reciprocating systems on microbial reduction: A systematic review. J Conserv Dent. 2019;22:114–122.
Janani K, Sandhya R. A survey on skills for cone beam computed tomography interpretation among endodontists for endodontic treatment procedure. Indian J Dent Res. 2019;30:834–838.
Siddique R, Sureshbabu NM, Somasundaram J, et al. Qualitative and quantitative analysis of precipitate formation following interaction of chlorhexidine with sodium hypochlorite, neem, and tulsi. J Conserv Dent. 2019; 22:40–47.
Rajendran R, Kunjusankaran RN, Sandhya R, et al. Comparative evaluation of remineralizing potential of a paste containing bioactive glass and a topical cream containing casein phosphopeptide-amorphous calcium phosphate: An in vitro study. Pesqui Bras Odontopediatria Clin Integr. 2019;19:1–10.
Govindaraju L, Neelakantan P, Gutmann JL. Effect of root canal irrigating solutions on the compressive strength of tricalcium silicate cements. Clin Oral Investig. 2017; 21:567–571.
Khandelwal A, Palanivelu A. Correlation between dental caries and salivary albumin in adult population in chennai: An in vivo study. Bds. 2019;22:228–233.
Yu P, Wang C, Zhou J, et al. Influence of surface properties on adhesion forces and attachment of Streptococcus mutans to Zirconia in vitro. Biomed Res Int; 2016. DOI: 10.1155/2016/8901253
Epub ahead of print 15 November 2016.
O’Beirne JL, Shelton RM, Lumley PJ, et al. Influence of accelerated setting using plaster of Paris addition on long term properties of Portland cement based dental material. Advances in Applied Ceramics. 2011;110:157–161.
Verma A. Differences in bacterial colonization and biofilm formation property of uropathogens between the two most commonly used indwelling urinary catheters. Journal of Clinical and Diagnostic Research. Epub ahead of print; 2016. DOI: 10.7860/jcdr/2016/20486.7939.
Kim DH, Kwon T-Y. study of adhesion on composite resin coated with three surface sealants. Restor Dent Endod. 2017;42:39–47.
Astasov-Frauenhoffer M, Glauser S, Fischer J, et al. Biofilm formation on restorative materials and resin composite cements. Dental Materials. 2018;34:1702–1709.
Bollenl CML, Lambrechts P, Quirynen M. Comparison of surface roughness of oral hard materials to the threshold surface roughness for bacterial plaque retention: A review of the literature. Dental Materials. 1997;13:258–269.
Virdi M. Emerging Trends in Oral Health Sciences and Dentistry. BoD – Books on Demand; 2015.
Hansel C, Leyhausen G, Mai UEH, et al. Effects of various resin composite (Co) monomers and extracts on two caries-associated micro-organisms in vitro. Journal of Dental Research. 1998;77:60-67.
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