Association of dupA, iceA, homB Genes of Helicobacter pylori with Gastritis, Peptic Ulcer Disease and Gastric Cancer
Journal of Pharmaceutical Research International,
Objective: To determine the association of dupA, iceA, homB genes of Helicobacter pylori with gastro-duodenal diseases such as gastritis, peptic ulcer disease PUD and gastric cancer.
Materials and Methods: This cross-sectional analytical study was conducted at Gastroenterology Department, Shaikh Zayed Hospital Lahore. Patients with gastro-duodenal diseases and positive H. pylori were included. Gastric biopsies were taken from fundus, body and antrum. H. pylori DNA were removed utilizing Gentra DNA extraction Kit (Life Technologies, USA) as per the technique and Qualitative PCR for the recognition of H. Pylori DNA. The PCR primers sets were designed for the specific detection of dupA, iceA and homB genes of H. pylori. All the data was recorded in proforma and analyzed by SPSS version 20.
Results: Mean age of the cases was 41.22+8.04 years. Males were more affected 118(60.2%). HomB was the most common 76(38.8%) followed by dupA and iceA 28.6% and 24.5% respectively. Peptic ulcer disease and gastritis were higher among patients having dup A and iceA positive strains as compared to homB gene patients, while gastric cancer was significantly higher among HomB gene infected patients, p-values were quite significant.
Conclusion: It was concluded that homB gene was most frequent in H. pylori infected population. Peptic ulcer disease and gastritis are markedly associated with dupA and iceA genes, while homB gene infected patients are at high risk of gastric cancer.
- H. pylori
- homB genes
- gastric diseases.
How to Cite
Liu ZF, Chen CY, Tang W, Zhang JY, Gong YQ, Jia JH. Gene-expression profiles in gastric epithelial cells stimulated with spiral and coccoid Helicobacter pylori. J Med Microbiol. 2006;55:1009-1015.
Schubert ML, Peura DA. Control of gastric acid secretion in health and disease. Gastroenterology. 2008;134(7):1842-1860.
Suerbaum S, Michetti P. Helicobacter pylori infection. New England Journal of Medicine. 2002;347(15):1175-1186.
Oleastro M, et al. Helicobacter pylori virulence genotypes in Portuguese children and adults with gastroduodenal pathology. Eur J Clin Microbiol Infect Dis. 2003;22: 85-91.
Tsuji S, Kawai N, Tsujii M, Kawano S, Hori M. inflammation‐related promotion of gastrointestinal carcinogenesis–a perigenetic pathway. Alimentary Pharmacology & Therapeutics. 2003;18: 82-89.
Suganuma M, Yamaguchi K, Ono Y, Matsumoto H, Hayashi T, Ogawa T, Imai K, Kuzuhara T, Nishizono A, Fujiki H. TNF‐α‐inducing protein, a carcinogenic factor secreted from H. pylori, enters gastric cancer cells. International Journal of Cancer. 2008;123(1):117-122.
Arachchi HJ, Kalra V, Lal B, Bhatia V, Baba CS, Chakravarthy S, Rohatgi S, Sarma PM, Mishra V, Das B, Ahuja V. Prevalence of duodenal ulcer‐promoting gene (dupA) of Helicobacter pylori in patients with duodenal ulcer in North Indian population. Helicobacter. 2007; 12(6):591-597.
Oleastro M. Disease association with two Helicobacter pylori duplicate outer membrane protein genes, homB and homA. Gut Pathog. 2009;1(1):12.
Jung SW, Sugimoto M, Graham DY, Yamaoka Y. homB status of Helicobacter pylori as a novel marker to distinguish gastric cancer from duodenal ulcer. J Clin Microbiol. 2009;47(10):3241-3245.
Wroblewski LE, Peek RM, Wilson KT. Helicobacter pylori and gastric cancer: factors that modulate disease risk. Clinical Microbiology Reviews. 2010;23(4):713- 739.
Agah S, Khedmat H, Ghamar-Chehreh ME, et al. Female gender and Helicobacter Pylori Infection, the most important predisposition factors in a cohort of gastric cancer. A longitudinal study. Caspian J Intern Med. 2016;7(2):136-141.
Abadi AT, Rafiei A, Ajami A, Hosseini V, Taghvaei T, Jones KR, Merrell DS. Helicobacter pylori homB, but not cagA, is associated with gastric cancer in Iran. Journal of Clinical Microbiology. 2011; 49(9):3191-3197.
Jung SW, Sugimoto M, Graham DY, Yamaoka Y. homB status of Helicobacter pylori as a novel marker to distinguish gastric cancer from duodenal ulcer. Journal of Clinical Microbiology. 2009; 47(10):3241-3245.
Kang J, Jones KR, Jang S, Olsen CH, Yoo YJ, Merrell DS, Cha JH. The geographic origin of Helicobacter pylori influences the association of the homB gene with gastric cancer. Journal of Clinical Microbiology. 2012;50(3):1082-1085.
Tsang TK, Shrestha MP. Helicobacter Pylori Infection in Peptic Ulcer Disease. Peptic Ulcer Disease. 2011;39.
Lu H, Hsu PI, Graham DY, Yamaoka Y. Duodenal ulcer promoting gene of Helicobacter pylori. Gastroenterology. 2005;128(4):833-848.
Shiota S, Matsunari O, Watada M, Hanada K, Yamaoka Y. Systematic review and meta-analysis: The relationship between the Helicobacter pylori dupA gene and clinical outcomes. Gut Pathog. 2010;2(1): 13.
Babaei V, Saghaei Y, Gohardani HZ, Vali F, Teimourian S. Effects of Different Environmental Factors and Virulence Factors, dupA and iceA Genes, of Helicobacter pylori on Peptic Ulcer. Jundishapur Journal of Microbiology. 2017; 10(5).
Momenah AM, Tayeb MT. Helicobacter pylori cagA and iceA genotypes status and risk of peptic ulcer in Saudi patients. Saudi Med J. 2007;28(3):382–385.
Ciftci IH, Uslan I, Dilek FH, Asik G, Ozgur MA, Dilek ON. Investigation of Helicobacter pylori iceA1 and iceA2 genes in patients with chronic gastritis and gastric cancer. Mikrobiyol Bul. 2011;45(2):228–233.
Abu-Taleb AM, Abdelattef RS, Abdel-Hady AA, Omran FH, El-Korashi LA, El- hady AA, El-Gebaly AM. Prevalence of Helicobacter pylori cagA and iceA genes and their association with gastrointestinal diseases. International Journal of Microbiology; 2018.
Ashour AA, Magalhaes PP, Mendes EN, Collares GB, de Gusmao VR, Queiroz DM, Nogueira AM, Rocha GA and de Oliveira CA. Distribution of vacA genotypes in Helicobacter pylori strains isolated from Brazilian adult patients with gastritis, duodenal ulcer or gastric carcinoma. FEMS Immunol Med Microbiol. 2002;33: 173-178.
Ben Mansour K, Fendri C, Zribi M, Masmoudi A, Labbene M, Fillali A, Ben Mami N, Najjar T, Meherzi A, Sfar T and Burucoa C. Prevalence of Helicobacter pylori vacA, cagA, iceA and oipA genotypes in Tunisian patients. Ann Clin Microbiol Antimicrob. 2010;9:10.
Van Doorn LJ, Figueiredo C, Sanna R, Plaisier A, Schneeberger P, de Boer W and Quint W. Clinical relevance of the cagA, vacA, and iceA status of Helicobacter pylori. Gastroenterology. 1998;115:58-66.
Peek RM Jr, Thompson SA, Donahue JP, Tham KT, Atherton JC, Blaser MJ, Miller GG. Adherence to gastric epithelial cells induces expression of a Helicobacter pylori gene, iceA, that is associated with clinical outcome. Proc Assoc Am Physicians. 1998;110:531-544.
Lu C, Yu Y, Li L, Yu C, Xu P. Systematic review of the relationship of Helicobacter pylori infection with geographical latitude, average annual temperature and average daily sunshine. BMC Gastroenterology. 2018;18(1):50.
Zhu Y, Zhou X, Wu J, Su J, Zhang G. Risk factors and prevalence of Helicobacter pylori infection in persistent high incidence area of gastric carcinoma in Yangzhong city. Gastroenterology Research and Practice; 2014.
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