The Content, Purification Degree, and Molecular Weight of Inulin of Natural Dangshen Roots (Codonopsis javanica) in Highland Lam Vien, Vietnam

Main Article Content

Nguyen Thi Thang Long
Vu Ngoc Boi
Dang Xuan Cuong

Abstract

Introduction: Inulins are a group of natural active polysaccharides found in ginseng and dangshen and mainly used in pharmaceutical preparations and functional food. The purification condition of inulin from dangshen grown in Vietnam did not occur in previous studies. Hence, the study presented on the content, purification degree, molecular weight, and functional group characteristics of inulin extracted from natural dangshen roots (Codonopsis javanica) in other purification conditions.

Methods: Some factors survey impact purification conditions of inulin, for example, the kinds and the concentration of the solvent, the temperature and the times of the precipitation, and the active coal impact. The objects such as the content of inulin, fructan, and crude polysaccharide, purification degree of inulin, and fructan were analyzed. Molecular weight and functional group characteristics of purification inulin extracted from dangshen roots (Codonopsis javanica) grown in highland Lam Vien, Vietnam, were also analyzed.

Results: The purification degree of inulin purified by using ethanol was higher than that using active coal, corresponding to 97.85a ± 0.84 % and 94.05 %, respectively. Inulin content was the large ratio in crude polysaccharide, exhibited via FTIR and the analysis results. Inulin and fructan were the most precipitated in ethanol, for example, 80 % and 90 % ethanol, respectively, compared to another solvent. The kinds and the concentration of the solvent, the temperature and the precipitation times, and active coal impacted the content and the purification degree of inulin and fructan (p < 0.05). The precipitation of inulin and fructan in ethanol solvent was affected by the temperature. The molecular weight of inulin and fructan corresponded to 3,193 Da and 1,112,892 Da, respectively.

Conclusion: Inulin of natural dangshen roots in Lam Vien highland, Vietnam was useful for functional foods and pharmaceutics.

Keywords:
Inulin, fructan, dangshen, Codonopsis javanica, purification.

Article Details

How to Cite
Long, N. T. T., Boi, V. N., & Cuong, D. X. (2020). The Content, Purification Degree, and Molecular Weight of Inulin of Natural Dangshen Roots (Codonopsis javanica) in Highland Lam Vien, Vietnam. Journal of Pharmaceutical Research International, 32(24), 83-92. https://doi.org/10.9734/jpri/2020/v32i2430813
Section
Original Research Article

References

Shoaib M, Shehzad A, Omar M, Rakha A, Raza H, Sharif HR, Shakeel A, Ansari A, Niazi S. Inulin: Properties, health benefits and food applications. Carbohydr Polym. 2016;147:444-454.

Advax A. A potent and safe immunopotentiator composed of delta inulin. Immunopotentiators in Modern Vaccines. Immunopotentiators in Modern Vaccines. 2017;199-210.

Barclay T, Ginic MM, Cooper P, Petrovsky N. Inulin-a versatile polysaccharide with multiple pharmaceutical and food chemical uses. J Excip Food Chem. 2016;1(3):1132.

Chang R. Bioactive polysaccharides from traditional Chinese medicine herbs as anticancer adjuvants. J Altern Complem Med. 2002;8(5):559-565.

Nadezhda P, Denev Panteley. Evaluation of fructan contents in the taproots of plants Lactuca serriola L. and Sonchus oleraceus L. Scientific Bulletin Series F Biotechnologies. 2013;17:117-122.

Elok Z, Wilda A. Comparative study of inulin extracts from dahlia, yam, and gembili tubers as prebiotic. Food Sci Nutr. 2013;4(11):8-12.

Kelly Greg. Inulin-type prebiotics: A review. (Part 2). Alternative medicine review: Clin Ther. 2009;14(1):36-55.

Flamm G, Glinsmann W, Kritchevsky D, Prosky L, Roberfroid M. Inulin and oligofructose as dietary fiber: A review of the evidence. Food Sci Nutr. 2001;41(5): 353-362.

Marcel BR. Inulin-type fructans: Functional food ingredients. J Nutr. 2007;137(11 Suppl):2493S-2502S.

Scholz-Ahrens KE, Adolphi B, Rochat F, Barclay DV, De Vrese M, Açil Y, Schrezenmeir J. Effects of probiotics, prebiotics, and synbiotics on mineral metabolism in ovariectomized rats—impact of bacterial mass, intestinal absorptive area and reduction of bone turn-over. NFS Journal. 2016;3:41-50.

Mensink MA, Frijlink HW, van der Voort Maarschalk K, Hinrichs WL. Inulin, a flexible oligosaccharide I: Review of its physicochemical characteristics. Carbohydr Polym. 2015;130:405-419.

Apolinário AC, Lima DB, Ponciano G, Macêdo B, Napoleão E, Pessoa A, Converti A, Silva JA. Inulin-type fructans: A review on different aspects of biochemical and pharmaceutical technology. Carbohydr Polym. 2014;101:368-378.

Anil KG, Navdeep K, Kaur N. Preparation of inulin from chicory roots. JSIR. 2003; 62(09):916-920.

Xu J, Yue R-Q, Liu J, Ho H-M, Yi T, Chen H-B, Han Q-B. Structural diversity requires individual optimization of ethanol concentration in polysaccharide precipitation. Int J Biol Macromol. 2014;67: 205-209.

Reza K, Hossein AM, Mehran G, Vaziri Moharam. Application of inulin in cheese as prebiotic, fat replacer and texturizer: A review. Carbohydr Polym. 2015;119:85-100.

JTCL T, KJ P, JRP R, FEX M, Fabbro IMD. Rheological characterization of chicory root (Cichorium intybus L.) inulin solution. Braz J Chem Eng. 2008;25(3):461-471.

Nielsen S. Food analysis laboratory manual. Second Edition ed. USA: University West Lafayette. Springer Publishing. 2010;117.

DuBois M, Gilles EKA, Hamilton JK, Rebers PAJ, Smith F. Calorimetric dubois method for determination of sugar and related substances. Analytical Chemistry. 1956;28(3):350-356.

Pencheva D, Petkova N, Denev P. Determination of inulin in dough products. Scientific Works of UFT. 2012;LIX:339-344.

Yevtifieieva ОА, Proskurina KI, Smelova NN, Petukhova IY. Development and validation of UV-Spectrophotometric method for quantitative determination of inulin by specific absorbance. Der Pharma Chemica. 2016;8(1):213-222.

Jun X, Rui-Qi Y, Jing L, Hing-Man H, Tao Y, Hu-Biao C, Quan-Bin H. Structural diversity requires individual optimization of ethanol concentration in polysaccharide. Int. J. Biol. Macromol. 2014;67:205–209.

Nadezhda P, Manol O, Mina T, Denev Panteley. Ultrasound-assisted extraction and characterisation of inulin-type fructan from roots of elecampane (Inula helenium L.). Acta Scientifica Naturalis. 2015;1(1): 225-235.

Khuenpet K, Fukuoka M, Jittanit W, Sirisansaneeyakul S. Spray drying of inulin component extracted from Jerusalem artichoke tuber powder using conventional and ohmic-ultrasonic heating for extraction process. J Food Eng. 2017;194:67-78.

John C. Interpretation of Infrared Spectra, A Practical Approach. In: Robert AM, editor. Encyclopedia of Analytical Chemistry. New York, United States. John Wiley & Sons; 2007.

Aurea BN, Gerardo AG, Norma G, José LMS, María dlÁVV, Leopoldo GC. Fourier transform infrared and Raman spectroscopic study of the effect of the thermal treatment and extraction methods on the characteristics of ayocote bean starches. J Food Sci Technol. 2017;54(4): 933–943.

Rengasamy RRK, Rajasekaran A, Perumal A. Fourier transform infrared spectroscopy analysis of seagrass polyphenols. Current Bioactive Compounds. 2011;7:118-125.

Luca P. Advances in nanoparticles: Synthesis, characterization, theoretical modelling, and applications. Switzerland: MDPI. 2020;144.

Jakubczyk E, Ostrowska-Lige˛za E, Gondek E. Moisture sorption characteristics and glass transition temperature of apple puree powder. Int J Food Sci Technol. 2010;45:2515-2523.

Amir P, Mohammad BHN, Mohammad HHK, Mohammad HK. Serish inulin and wheat biopolymers interactions in model systems as a basis for understanding the impact of inulin on bread properties: A FTIR investigation. J Food Sci Technol. 2015;52(12):7964–7973.