Assessment of Antidiarrheal, Antispasmodic and Antimicrobial Activities of Methanolic Seeds Extract of Peganum harmala L. (Nitrariaceae)

Main Article Content

Mohd Nazam Ansari

Abstract

Background: Many plants and herbs have been shown to possess antidiarrheal, antispasmodic, and antimicrobial activities. The present study was developed to determine the possible antidiarrheal and antispasmodic effects of methanolic extract of Peganum harmala L. (MEPH) in diarrhea and hyperactive gut.

Methodology: The crude MEPH was studied using the in-vivo castor oil-induced diarrhea model in mice, while isolated rat ileum was used in the in-vitro studies. Antimicrobial efficacy of MEPH was tested against different bacterial strains (Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Klebsiella pneumonia), Yeast (Candida albicans) and Fungus (Aspergillus niger) using in-vitro assays.

Results: In the castor oil-induced diarrhea, MEPH at 100 and 200 mg/kg showed 40% and 80% protection in mice while positive control drug, loperamide showed 100% protection. In the in-vitro experiments, MEPH dose-dependently (0.01 to 0.3 mg/mL) inhibited carbachol and high K+-induced contractions at comparable EC50 values similar to papaverine, a phosphodiesterase (PDE) inhibitor. The PDE inhibitory like effect was further confirmed when pre-incubated ileum tissues with MEPH (0.1 and 0.3 mg/mL) shift the isoprenaline-mediated inhibitory CRCs against carbachol to the left, similar to papaverine. In antibacterial assay, MEPH showed efficacy against two Gram positive bacteria (S. aureus, and B. subtilis) and one Gram negative bacteria (E. coli). But extract didn’t show any effect against K. pneumoniae. The MEPH also showed efficacy against fungal strain (A. niger) and yeast strain (C. albicans).

Conclusions: MEPH shows antidiarrheal and antispasmodic effects because of its antimotility effect which were possibly due to the inhibition of PDE enzyme. The study has shown an optimal antimicrobial activity of MEPH as all bacteria, yeast and fungal strains were found sensitive except K. pneumoniae. MEPH proved efficacy against both enteric and non-enteric pathogens causing diarrhea, thus confirming its role in both the infectious and non-infectious diarrhea.

Keywords:
Syrian rue, hyperactive gut disorder, diarrhea, antibacterial, PDE inhibition

Article Details

How to Cite
Ansari, M. N. (2020). Assessment of Antidiarrheal, Antispasmodic and Antimicrobial Activities of Methanolic Seeds Extract of Peganum harmala L. (Nitrariaceae). Journal of Pharmaceutical Research International, 32(24), 74-82. https://doi.org/10.9734/jpri/2020/v32i2430812
Section
Original Research Article

References

Al-Bari MA, Sayeed MA, Rahman MS, Mossadik MA. Characterization and antimicrobial activities of a phenolic acid derivative produced by Streptomyces bangladeshiensis a novel species collected in Bangladesh. Res J Med Sci. 2006;1: 77-81.

Robin EH, Anril W, Alexander M, Loeto M, Keith K. Nasopharyngeal carriage and antimicrobial resistance in isolates of Streptococcus pneumonia and Haemophilus influenzae Type b in children under 5 years of age in otswana. Int J Infect Dis. 1998;3(1):18-25.

Tumah H. Fourth-generation cephalosporins: In vitro activity against nosocomial gram-negative bacilli compared with β-lactam antibiotics and ciprofloxacin. Chemotherapy. 2005;51(2-3):80-5.

Aslam B, Wang W, Arshad MI, Khurshid M, Muzammil S, Rasool MH, et al. Antibiotic resistance: a rundown of a global crisis. Infect Drug Resist. 2018;11:1645-58.

Özçelik B, Aslan M, Orhan İ, Karaoğlu T. Antibacterial, antifungal, and antiviral activities of the lipophilic extracts of Pistacia vera. Microbiol Res. 2005;160: 159-64.

Djeridane A, Yousfi M, Nadjemi B, Boutassouna D, Stocker P, Vidal N. Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chem. 2006; 97:654-60.

Cheesman MJ, Ilanko A, Blonk B, Cock IE. Developing New Antimicrobial Therapies: Are Synergistic Combinations of Plant Extracts/Compounds with Conventional Antibiotics the Solution? Pharmacogn Rev. 2017;11(22): 57-72.

Shamsa F, Monsef HR, Ghamooghi R, Verdian Rizi MR. Spectrophotometric determination of total alkaloids in Peganum harmala L. using bromocresol green. Res J Phytochem. 2007;1:79- 82.

Goel N, Singh N, Saini R. Efficient in vitro multiplication of Syrian Rue (Peganum harmala L.) using 6-benzylaminopurine pre-conditioned seedling explants. Nat Sci. 2009;7:129-34.

Moloudizargari M, Mikaili P, Aghajanshakeri S, Asghari MH, Shayegh J. Pharmacological and therapeutic effects of Peganum harmala and its main alkaloids. Pharmacogn Rev. 2013;7(14): 199-212.

Shapira Z, Terkel J, Egozi Y, Nyska A, Friedman J. Abortifacient potential for the epigeal parts of Peganum harmala. J Ethnopharmacol. 1989;27:319–25.

Nenaah G. Antibacterial and antifungal activities of (beta)-carboline alkaloids of Peganum harmala (L) seeds and their combination effects. Fitoterapia. 2010;81: 779–82.

Saadabi AM. Antifungal activity of some Saudi plants used in traditional medicine. Asian J Plant Sci. 2006;5: 907–9.

Akhtar MS, Iqbal Z, Khan MN, Lateef M. Anthelmintic activity of medicinal plants with particular reference to their use in animals in the Indo±Pakistan subcontinent. Small Rumin Res. 2000;38:99–107.

National Research Council, Guide for the Care and Use of Laboratory Animals, National Academy Press, Washington, 1996;1-7.

Rehman NU, Ansari MN, Samad A. In silico, ex-vivo and in-vivo studies of Roflumilast as potential Antidiarrheal and Antispasmodic agent: Inhibition of PDE-4 Enzyme and Voltage-gated Ca++ ion Channels. Molecules. 2020;25(4):1008.

Rang HP, Dale MM, Ritter JM. Pharmacology, 4th edn. Churchill Livingstone, New York. 1999;289-90.

Sacchetti G, Maietti S, Muzzoli M, Scaglianti M, Manfredini S, Radice M, et al. Comparative evaluation of 11 essential oils of different origin as functional antioxidants, antiradicals and antimicrobials in foods. J Food Chem. 2005;91:621-32.

Celiktas OY, Kocabas EEH, Bedir E, Sukan FV, Ozek T, Baser KHC. Antimicrobial activities of methanol extracts and essential oils of Rosmarinus officinalis, depending on location and seasonal variations. J Food Chem. 2007;100: 553-9.

Jenkins SG, Schuetz AN. Current concepts in laboratory testing to guide antimicrobial therapy. Mayo Clin Proc. 2012;87(3):290-308.

Iwao I, Terada Y. On the mechanism of diarrhea due to castor oil. Jap J Pharmacol. 1962;12:137-45.

Croci T, Landi M, Elmonds-Alt X, Le-Fur G, Maffrand JP, Manara L. Role of tachykinins in castor oil-induced diarrhoea in rats. Br J Pharmacol. 1997;121:375–80.

Rehman NU, Gilani AH, Khan A, Nazneen M, El Gamal AA, Fawzy GA, et al. Antidiarrheal and Antispasmodic Activities of Buddleja polystachya are Mediated Through Dual Inhibition of Ca(++) Influx and Phosphodiesterase Enzyme. Phytother Res. 2015;29(8):1211-8.

Palla AH, Sibhat GG, Karim A, Rehman NU, Hiben MG. Multiple Pathway–Mediated Gut-Modulatory Effects of Maerua subcordata (Gilg) DeWolf. J Exp Pharmacol. 2020;12:203-11.

Gilani AH, Khan A, Subhan F, Khan M. Antispasmodic and bronchodilator activities of St. John’s wort are putatively mediated through dual inhibition of calcium influx and phosphodiesterase. Fundam Clin Pharmacol. 2005;19:695-705.

Choo LK, Mitchelson F. Antagonism of cholinomimetics by troxy pyrrolidinium in guinea-pig atria and longitudinal ileal muscle: comparison with hemicholinium-3. Eur J Pharmacol. 1978;52(3):313-22.

Kaneda T, Takeuchi Y, Matsui H, Shimizu K, Urakawa N, Nakajyo S. Inhibitory mechanism of papaverine on carbachol-induced contraction in bovine trachea. J Pharmacol Sci. 2005;98:275-82.

Othman L, Sleiman A, Abdel-Massih RM. Antimicrobial Activity of Polyphenols and Alkaloids in Middle Eastern Plants. Front Microbiol. 2019;10:911.

Shahverdi AR, Monsef-Esfahani HR, Nickavar B, Bitarafan L, Khodaee S, Khoshakhlagh N. Antimicrobial activity and main chemical composition of two smoke condensates from Peganum harmala seeds. Z Naturforsch C J Biosci. 2005;60:707– 10.