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Medicinal plants are potential natural sources of biologically active substances that have geroprotective properties, slowing down the aging process. Vegetable raw materials are used as antioxidants in the food industry. Pulmonaria officinalis L. has a high content of biologically active substances. The purpose of this work is to determine the operating parameters for extracting the maximum amount of biologically active substances from Pulmonaria officinalis L. by extracting ethanol and finding sources of natural antioxidants.The antioxidant activity of several ethanol extracts of Pulmonaria officinalis L. – 30-70% with a step of 10 ethanol obtained from the dried root culture of the medicinal plant of biologically active substances was determined. The total content of polyphenols, flavonoids and proanthocyanidins was estimated. According to the data obtained, the relationship between the values determined for the biologically active substances presented and the values of antioxidant activity was revealed. Extracts of Pulmonaria officinalis L. showed a high yield of polyphenolic compounds-889.39±4.29 mcg of Gallic acid/ml, flavonoids-728.90±6.98 mcg of rutin/ml, proanthocyanidins-211.65±5.31 mcg of catechin/ml at an ethyl alcohol concentration of 60% and an extraction temperature of 50°C in 4 hours. The antioxidant activity using 2 methods: A spectrophotometric method DPPH (2,2-diphenyl-1-picrylhydrazyl) and the FRAP method (iron reducing/antioxidant power) was investigated. During DPPH spectrophotometric analysis, the antioxidant activity is equal to 86.96% for polyphenols, 75.47% for flavonoids, and 51.25% for proanthocyanidins. FRAP analysis showed that extracts with operating parameters had a pronounced antioxidant activity (tэ= 4 ч; Tэ= 50°C; Сэ= 60%)и (tэ= 5 ч; Tэ= 60°C; Сэ, = 70%).
Dang LAA, Fadzil NHM, Jamaluddin A, Rashid NYA, Musaalbakri AM. Effects of different extracting conditions on anti-tyrosinase and antioxidant activities of Schizophyllum commune fruit bodies/ Biocatalysis and Agricultural Biotechnology. 2019;19:101116.
Lin JY, Tang CY. Determination of total phenolic and flavonoid contents in selected fruits and vegetables, as well as their stimulatory effects on mouse splenocyte proliferation. Food Chem. 2007;101:140–147.
Kamble SS, Gacche RN. Evaluation of anti-breast cancer, anti-angiogenic and antioxidant properties of selected medicinal plants. European Journal of Integrative Medicine. 2019;25:13–19.
Ghuman S, Ncube B, Finnie JF, McGaw LJ, Van Staden J. Antioxidant, anti-inflammatory and wound healing properties of medicinal plant extracts used to treat wounds and dermatological disorders South African. Journal of Botany 2019;126:232–240.
Harhaun R, Kunik O, Saribekova D, Lazzara G. Biologically active properties of plant extracts in cosmetic emulsions. Microchemical Journal. 2020;154:104543.
Alseekh S, Souza LP, Benina M, Fernie AR. The style and substance of plant flavonoid decoration; towards defining both structure and function. Phytochemistry. 2020;174:112347.
Zalesak F, Bon DJYD, Pospísil J. Lignans and Neolignans: Plant secondary metabolites as a reservoir of biologically active substances. Pharmacological Research. 2019;146: 104284.
Bubalo MS, Vidovic S, Redovnikovic IR, Jokic S. New perspective in extraction of plant biologically active compounds by green solvents. Food and Bioproducts Processing. 2018;109:52–73.
He JL, Guo H, Wei SY, Zhou J. Effects of different extraction methods on the structural properties and bioactivities of polysaccharides extracted from Qingke (Tibetan hulless barley). Journal of Cereal Science. 2020;92: 102906.
Johner CF, Tahmasb H, Angela M, Meireles A. Developing a supercritical fluid extraction method assisted by cold pressing for extraction of pequi (Caryocar brasiliense). The Journal of Supercritical Fluids. 2018;137:34–39.
Ayyildiz SS, Karadeniz B, Sagcan N, Bahar B. Optimizing the extraction parameters of epigallocatechin gallate using conventional hot water and ultrasound assisted methods from green tea. Food and Bioproducts Processing. 2018;111:37–44.
Yuan Z, Li G, Wei W, Wang J, Fang Z. A comparison of different pre-extraction methods followed by steam pretreatment of bamboo to improve the enzymatic digestibility and ethanol production. Energy. 2020;1961: 117156.
Oreopoulou A, Tsimogiannis D, Oreopoulou V. Extraction of polyphenols from aromatic and medicinal plants: an overview of the methods and the effect of extraction parameters. Polyphenols in Plants (Second Edition). 2019;25(2):243–259.
Sourki H, Koocheki A, Elahi M. Ultrasound-assisted extraction of β-d- glucan from hull-less barley: assessment of physicochemical and functional properties. Int. J. Biol. 2017;95:462–475.
Smotraeva IV, Balanov PE, Tretyakov NA. Application of ultrasound in the processing of plant raw materials. Proceedings of the Saint Petersburg State Agrarian University. 2014;37:264–267.
Oliveira C.F., Giordani D., Gurak PD, Cladera-Olivera F, Damasceno L. Extraction of pectin from passion fruit peel using moderate electric field and conventional heating extraction methods / Innovative Food Science & Emerging Technologies. 2015;29:201-208.
Rui MA, Fan C, Wang Y, Lo J, Komarneni S. Gas-liquid extraction in a new rotating Microchannel extractor. Journal Pre-proof. 2020;54(5):258–296.
Sorescu AA, Nuta A, Ion RM, Nistor CL, Ghiurea M. Biosynthesis of noble metallic nanoparticles from Pulmonaria officinalis. International Multidisciplinary Scientific GeoConference SGEM. 2019;19:61–68.
Polukhina TV, Nurgalaev GB. Study of the quantitative content of ascorbic acid in the aboveground part of the medicinal honeydew (Pulmonaria officinalis). Basic and applied scientific research. 2017;1: 243–245.
Lazareva SV. Pharmacological study of medicinal honeydew. Smolensk Medical Almanac. 2018;2:4–7.
Adewusi AEA, Steenkamp V. In vitro screening for acetylcholinesterase inhibition and antioxidant activity of medicinal plants from southern Africa. Asia Pac. J. Trop. Med. 2011;4(11):829–835.
Adewusi AEB, Moodley N, Steenkamp V. Medicinal plants with cholinesterase inhibitory activity: A review. J. Biotechnol. 2010;9:8257–8276.
Al-Ansari M, Al-Humaid LA, Vijayaraghavan P, Ravindran B, Balamuralikrishnan B. Identification of phytochemical components from Aerva lanata (Linn.) medicinal plants and its in-vitro inhibitory activity against drug resistant microbial pathogens and antioxidant properties. Saudi Journal of Biological Sciences. 2019;26(6):1129-1133.
Eruygur N, Ucar E, Akpulat HA, Shahsavari K, Safavi SM, Kahrizi D. In vitro antioxidant assessment, screening of enzyme inhibitory activities of methanol and water extracts and gene expression in Hypericum lydium. Molecular Biology Reports. 2019;46(2):2121–2129.
Gan RY, Song FL, Kuang L, Li HB, Xu XR. Antioxidant activity and total phenolic content of medicinal plants associated with prevention and treatment of cardiovascular and cerebrovascular diseases. Journal of Medicinal Plants Research. 2010;4(22): 2438–2444.
Ghuman S, Ncube B, Finnie JF, McGaw LJ, Van Staden J. Antioxidant, anti-inflammatory and wound healing properties of medicinal plant extracts used to treat wounds and dermatological disorders South African / Journal of Botany. 2019;126:232–240.
Solovyova NA, Khizhnyak SD, Pakhomov PM. Investigation of the qualitative composition and antioxidant activity of phenolic compounds of common yarrow in the conditions of industrial pollution of the city of Tver. Bulletin of Tver State University. Series: Chemistry. 2015;4: 102–111. Available:https://www.elibrary.ru/item.asp?id=25416836
Neagu E, Radu GL, Gabriela FC. Antioxidant activity, acetylcholinesterase and tyrosine inhibitory potential of Pulmonary officinal is and Centrum umbellate extracts. Saudi Journal of Biological Sciences. 2018;25(3):578– 585.
Bystrova EA, Alekseenko EV. Investigation of the component composition of phenolic compounds and the antioxidant activity of cranberry juice. University news. Applied chemistry and biotechnology. 2017;3(22): 19–26.
Velasco L, Goffman FD. Chemotaxonomic significance of fatty acids and tocopherols in Boraginaceae. Phytochemistry. 1999; 52(3):423–426.
Chirinos R, Pedreschi R, Rogez H, Larondelle Y, Campos D. Phenolic compound contents and antioxidant activity in plants with nutritional and/or medicinal properties from the Peruvian Andean region. Industrial Crops and Products. 2013;47:145–152.
Meeus S, Janssens S, Helsen K, Jacquemyn H. Evolutionary trends in the distylous genus Pulmonaria (Boraginaceae): Evidence of ancient hybridization and current interspecific gene flow. Molecular Phylogenetics and Evolution. 2016;98:63–73.
Fale PLA, Amaral F, Madeira PJ, Silva SM, Florencio MH, Frazao FN, Serralheiro MLM. Acetylcholinesterase inhibition, antioxidant activity and toxicity of Peumus boldus water extracts on HeLa and CaCo-2 cell lines. Food Chem. Toxicol. 2012; 50:2656–2662.
Ferreira A, Proenc C, Serralheiro MLM, Araujo MEM. The in vitro screening for acetylcholinesterase inhibition and antioxidant activity of medicinal plants from Portugal. J. Ethnopharmacol. 2018;15:31–37.
Greggio E, Bergantino E, Carter D, Ahmad R, Costin GE, Hearing VJ, etc. Tyrosine exacerbates dopamine toxicity but is not genetically associated with Parkinson’s disease. J. Neurochem. 2005;93:246– 256.
Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. 1999;299:152–178.
Butler LG, Price ML. Vanilin assay for proanthocyanidins (condensed tannins) modification of the solvent for estimation of the degree of polymerization. J. Agric. Food Chem. 1982;30:1087–1089.
Bondet V, Brand-Williams W, Berset C. Kinetics and mechanisms of antioxidant activity using the DPPH free radical method. Lebensm. Wiss. Technol. 1999; 30:609.
Berker K, Guclu K, TorI, Apak R. Comparative evaluation of Fe(III) reducing power-based antioxidant capacity assays in the presence of phenanthroline, batho-phenanthroline, tripyridyltriazine (FRAP), and ferricyanide reagents . Talanta. 2007; 72:1157–1165.
Rajurkar NS, Hande SM. Estimation of phytochemical content and antioxidant activity of some selected traditional Indian medicinal plants. Indian Journal of Pharmaceutical Sciences. 2011;73(2): 146.