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This study aimed to identify, evaluate the antibiotic resistance pattern and detect virulence genes iss, and ompT in avian pathogenic Escherichia coli (APEC) from broiler chickens in central Nepal. To determine the antibiotic resistance pattern of the obtained isolates, the Kirby-Bauer disc diffusion method was used with six different commercial antibiotic discs: Amikacin, Gentamycin, Ciprofloxacin, Doxycycline, Chloramphenicol and Levofloxacin. A polymerase chain reaction (PCR) assay was used for the selected isolates (n=40) to screen the presence of the iss and ompT genes after the extraction of DNA using the boiling method Out of 60 suspected Colibacillosis liver samples, 40 were confirmed as E. coli positive The antibiogram profile revealed maximum resistance to Doxycycline (87.5%), followed by Levofloxacin (72.5%), Ciprofloxacin (67.5%), Chloramphenicol (40.0%), Gentamycin (32.5%) and Amikacin (10.0%).. The presence of the iss and ompT genes was found to be 100.0% and 90.0%, respectively. APEC was found to be highly resistant to most of the antibiotics. Virulence-associated genes iss and ompT were obtained at high percentages from Colibacillosis suspected broiler chickens in Chitwan, Nepal. These finding suggests that the judicial use of antimicrobials is compulsory to check antibiotic resistance and Colibacillosis outbreaks in poultry farms.
Lamarche MG, Dozois CM, Daigle F, Caza M, Curtiss R, Dubreuil JD, Harel J. Inactivation of the pst system reduces the virulence of an avian pathogenic Escherichia coli O78 strain. Infection and Immunity. 2005;73(7):4138-45.
De Carli S, Ikuta N, Lehmann FK, da Silveira VP, de Melo PG, Fonseca AS, Lunge VR. Virulence gene content in Escherichia coli isolates from poultry flocks with clinical signs of Colibacillosis in Brazil. Poultry Science. 2015;94(11):2635-40.
Matin MA, Islam MA, Khatun MM. Prevalence of Colibacillosis in chickens in greater Mymensingh district of Bangladesh. Veterinary World. 2017;10(1): 29.
Matter LB, Barbieri NL, Nordhoff M, Ewers C, Horn F. Avian pathogenic Escherichia coli MT78 invades chicken fibroblasts. Veterinary Microbiology. 2011;148(1):51-9.
Shrestha A, Bajracharya AM, Subedi H, Turha RS, Kafle S, Sharma S, Neupane S, Chaudhary DK. Multi-drug resistance and extended spectrum beta lactamase producing Gram negative bacteria from chicken meat in Bharatpur Metropolitan, Nepal. BMC Research Notes. 2017;10(1): 1-5.
Aarestrup FM. Veterinary drug usage and antimicrobial resistance in bacteria of animal origin. Basic & Clinical Pharmacology & Toxicology. 2005;96(4): 271-81.
Gyles CL. Antimicrobial resistance in selected bacteria from poultry. Animal Health Research Reviews. 2008;9(2):149.
EMEA. Antibiotic resistance in the European Union associated with therapeutic use of veterinary medicines. Report and qualitative risk assessment by the Committee for Veterinary Medical Products, The European Agency for the evaluation of Medical products; 1999. Available:https://www.ema.europa.eu/en/documents/report/antibiotic-resistance-european-union-associated-therapeutic-use-veterinary-medicines-report_en-0.pdf Accessed September 2, 2020.
Miles TD, McLaughlin W, Brown PD. Antimicrobial resistance of Escherichia coli isolates from broiler chickens and humans. BMC Veterinary Research. 2006;2(1):7.
Umolu PI, Ohenhen ER, Okwu IG, Ogiehor IS. Multiple antibiotic resistant index and plasmid of Escherichia coli in beef in Ekpoma. J. Am. Sci. 2006;2(3):22-8.
Centres for Disease Control and Prevention (US). Antibiotic resistance threats in the United States, 2013. Centres for Disease Control and Prevention, US Department of Health and Human Services; 2013.
Rodriguez-Siek KE, Giddings CW, Doetkott C, Johnson TJ, Nolan LK. Characterizing the APEC pathotype. Veterinary Research. 2005;36(2):241-56.
Badouei MA, Jajarmi M, Mirsalehian A. Virulence profiling and genetic relatedness of Shiga toxin-producing Escherichia coli isolated from humans and ruminants. Comparative Immunology, Microbiology And Infectious Diseases. 2015;38:15-20.
Pfaff-McDonough SJ, Horne SM, Giddings CW, Ebert JO, Doetkott C, Smith MH, Nolan LK. Complement resistance-related traits among Escherichia coli isolates from apparently healthy birds and birds with colibacillosis. Avian Diseases. 2000; 23-33.
Hwang BY, Varadarajan N, Li H, Rodriguez S, Iverson BL, Georgiou G. Substrate specificity of the Escherichia coli outer membrane protease OmpP. Journal of Bacteriology. 2007;189(2):522-30.
Karki A, Tiwari BR, Pradhan SB. Study of bacteria isolated from urinary tract infection and their sensitivity pattern. Journal of the Nepal Medical Association. 2004;43(154).
Osti R, Bhattarai D, Chaudhary H, Singh V. Poultry production in Nepal: Characteristics, productivity and constraints. International Journal of Applied Sciences and Biotechnology. 2017;5(2):222-6.
Subedi M, Luitel H, Devkota B, Bhattarai RK, Phuyal S, Panthi P, Shrestha A, Chaudhary DK. Antibiotic resistance pattern and virulence genes content in avian pathogenic Escherichia coli (APEC) from broiler chickens in Chitwan, Nepal. BMC Veterinary Research. 2018;14(1):1-6.
Wayne PA. Clinical and Laboratory Standards Institute (CLSI): Performance standards for antimicrobial susceptibility testing: 24th informational supplement (M100- S23). CLSI; 2014.
Diana JE, Pui CF, Son R. Enumeration of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in fruit juices. International Food Research Journal. 2012;19(1):51.
Johnson TJ, Kariyawasam S, Wannemuehler Y, Mangiamele P, Johnson SJ, Doetkott C, Skyberg JA, Lynne AM, Johnson JR, Nolan LK. The genome sequence of avian pathogenic Escherichia coli strain O1: K1: H7 shares strong similarities with human extraintestinal pathogenic E. coli genomes. Journal of Bacteriology. 2007;189(8):3228-36.
Mohamed MA, Shehata MA, Rafeek E. Virulence genes content and antimicrobial resistance in Escherichia coli from broiler chickens. Veterinary Medicine International. 2014;2014.
Radwan IA, Salam HS, Abd-Alwanis AA, Al-Sayed MY. Frequency of some virulence associated genes among multidrug-resistant Escherichia coli isolated from septicemic broiler chicken. Int. J. Adv. Res. 2014;2(12):867-74.
Madadi MS, Ghaniei A, Zare P, Isakakroudi N. Antimicrobial susceptibility pattern of Escherichia Coli Isolates to Antibacterial Agents in Urmia, Iran. Int J Basic Sci Res. 2014; 3:695-7.
Li Y, Chen L, Wu X, Huo S. Molecular characterization of multidrug-resistant avian pathogenic Escherichia coli isolated from septicemic broilers. Poultry Science. 2015;94(4):601-11.
Jin WJ, Zheng ZM, Zhang YZ, Qin AJ, Shao HX, Liu YL, Jiao WA, Wang QQ. Distribution of virulence-associated genes of avian pathogenic Escherichia coli isolates in China. Agricultural Sciences in China. 2008;7(12):1511-5.