Comparative Analysis of Antidyslipidemic Effects of Fenugreek Seed Extract and Standard Pharmacological Therapy in Diet Induced Animal Model of Dyslipidemia: An Experimental Study
Journal of Pharmaceutical Research International,
Page 143-151
DOI:
10.9734/jpri/2020/v32i2230782
Abstract
Objectives: The objective of this study was to compare the antidyslipidemic effects of fenugreek seed extract in comparison to standard drugs used for dyslipidemia in an animal model of dyslipidemia.
Methods: It was an experimental study conducted on 42 male, 9 weeks old Wistar albino rats for a period of 7 weeks. Animals were randomly divided into 7 groups, out of which 6 groups were given lipid rich diet to induce dyslipidemia for 28 days while one group served as control given normal diet. After 28 days standard drugs alone as well as in combinations (statins 10 mg/kg and fibrates 100 mg/kg) and fenugreek seed extract in two different doses, 250 mg/kg and 500 mg/kg were administered once a day for 21 days to all the respective groups except positive (lipid rich diet) and negative control groups (on normal diet), both were administered normal saline. Baseline body weights of all groups of animals were measured at the start of the study. At the end of the study period, body weights of all groups of animal groups were measured again and their blood was drawn through cardiac puncture for the assessment of TC, LDL-C, HDL, TGs, D/B, I/B and ALT. All the intervention groups were compared on the basis of above mentioned parameters and changes in weight.
Results: Amongst all the groups, lipid parameters (TC, LDL, TGs) showed a significant reduction and increase in HDL in the group on FSE in a dose dependent manner. Moreover, FSE also showed significant decrease in the levels of liver enzymes including ALT, D/B and in body weight when compared to other groups. While we did not find any significant change for urea, creatinine and I/B amongst all the groups.
Conclusion: It is concluded that the fenugreek seed extract showed superior effects when compared to traditional pharmacological therapy against dyslipidemia.
Keywords:
- Dyslipidemia
- lipid rich diet
- Atorvastatin
- Wistar albino rats
- Fenugreek seeds.
How to Cite
References
Collaboration PS. Blood cholesterol and vascular mortality by age, sex and blood pressure: A meta-analysis of individual data from 61 prospective studies with 55 000 vascular deaths. The Lancet. 2007;370:1829-1839.
Last AR, Ference JD, Menzel ER. Hyperlipidemia: Drugs for cardiovascular risk reduction in adults. American Family Physician. 2017;95:78-87.
Zodda D, Giammona R, Schifilliti S. Treatment strategy for dyslipidemia in cardiovascular disease prevention: Focus on old and new drugs. Pharmacy. 2018;6:10.
Gavronski M, Volmer D, Hartikainen S, Zharkovsky A. Potential drug interactions with statins: Estonian register-based study. Open Medicine. 2015;10.
Schultz BG, Patten DK, Berlau DJ. The role of statins in both cognitive impairment and protection against dementia: A tale of two mechanisms. Translational Neurodegeneration. 2018;7:5.
Björnsson ES. Hepatotoxicity of statins and other lipid‐lowering agents. Liver International. 2017;37:173-178.
Kheloussi S. Considerations in the approach to appropriate statin selection. US Pharmacist. 2018;43:22-26.
Maher RL, Hanlon J, Hajjar ER. Clinical consequences of polypharmacy in elderly. Expert Opinion on Drug Safety. 2014;13:57-65.
Nagulapalli Venkata KC, Swaroop A, Bagchi D, Bishayee A. A small plant with big benefits: Fenugreek (Trigonella foenum‐graecum Linn.) for disease prevention and health promotion. Molecular Nutrition & Food Research. 2017;61:1600950.
Kandhare AD, Thakurdesai PA, Wangikar P, Bodhankar SL. A systematic literature review of fenugreek seed toxicity by using ToxRTool: Evidence from preclinical and clinical studies. Heliyon. 2019;5:e01536.
Moosa ASM, Rashid MU, Asadi A, Ara N, Uddin MM, Ferdaus A. Hypolipidemic effects of fenugreek seed powder. Bangladesh Journal of Pharmacology. 2006;1:64-67.
Huang XS, Zhao SP, Bai L, Hu M, Zhao W, Zhang Q. Atorvastatin and fenofibrate increase apolipoprotein AV and decrease triglycerides by up‐regulating peroxisome proliferator‐activated receptor‐α. British Journal of Pharmacology. 2009;158:706-712.
Sharma MS, Choudhary PR. Effect of fenugreek seeds powder (Trigonella foenum-graecum L.) on experimental induced hyperlipidemia in rabbits. Journal of Dietary Supplements. 2017;14:1-8.
Ekor M. The growing use of herbal medicines: Issues relating to adverse reactions and challenges in monitoring safety. Frontiers in Pharmacology. 2014;4:177.
Kiss R, Pesti-Asbóth G, Szarvas MM, Stündl L, Cziáky Z, Hegedűs C, Kovács D, Badale A, Máthé E, Szilvássy Z. Diosgenin and its fenugreek based biological matrix affect insulin resistance and anabolic hormones in a rat based insulin resistance model. BioMed Research International; 2019.
Sharma MS, Choudhary PR. Hypolipidemic effect of fenugreek seeds and its comparison with atorvastatin on experimentally induced hyperlipidemia. J Coll Physicians Surg Pak. 2014;24:539-42.
Belguith-Hadriche O, Bouaziz M, Jamoussi K, El Feki A, Sayadi S, Makni-Ayedi F. Lipid-lowering and antioxidant effects of an ethyl acetate extract of fenugreek seeds in high-cholesterol-fed rats. Journal of Agricultural and Food Chemistry. 2010;58:2116-2122.
Vijayakumar MV, Pandey V, Mishra GC, Bhat MK. Hypolipidemic effect of fenugreek seeds is mediated through inhibition of fat accumulation and upregulation of LDL receptor. Obesity. 2010;18:667-674.
Takkella N, Konuri A, Kishore A, Rai KS, Bhat KM. Role of choline-docosahexaenoic acid and Trigonella foenum graecum seed extract on ovariectomy induced dyslipidemia and oxidative stress in rat model. Journal of Krishna Institute of Medical Sciences (JKIMSU). 2018;7.
Chaturvedi U, Shrivastava A, Bhadauria S, Saxena JK, Bhatia G. A mechanism-based pharmacological evaluation of efficacy of Trigonella foenum graecum (fenugreek) seeds in regulation of dyslipidemia and oxidative stress in hyperlipidemic rats. Journal of Cardiovascular Pharmacology. 2013;61:505-512.
Muraki E, Hayashi Y, Chiba H, Tsunoda N, Kasono K. Dose-dependent effects, safety and tolerability of fenugreek in diet-induced metabolic disorders in rats. Lipids in Health and Disease. 2011;10:240.
Uemura T, Goto T, Kang MS, Mizoguchi N, Hirai S, Lee JY, Nakano Y, Shono J, Hoshino S, Taketani K. Diosgenin, the main aglycon of fenugreek, inhibits LXRα activity in HepG2 cells and decreases plasma and hepatic triglycerides in obese diabetic mice. The Journal of Nutrition. 2011;141:17-23.
Nagamma T, Konuri A, Nayak CD, Kamath SU, Udupa PE, Nayak Y. Dose-dependent effects of fenugreek seed extract on the biochemical and haematological parameters in high-fat diet-fed rats. Journal of Taibah University Medical Sciences. 2019;14:383-389.
Taleb MH, Almasri IM, Siam NI, Najim AA, Ahmed AI. The effect of atorvastatin on liver function among patients with coronary heart disease in Gaza Strip; 2014.
Jose J. Statins and its hepatic effects: Newer data, implications and changing recommendations. Journal of Pharmacy & Bioallied Sciences. 2016;8:23.
Kandhare AD, Bodhankar SL, Mohan V, Thakurdesai PA. Glycosides based standardized fenugreek seed extract ameliorates bleomycin-induced liver fibrosis in rats via modulation of endogenous enzymes. Journal of Pharmacy & Bioallied Sciences. 2017;9:185.
Kumar P, Bhandari U, Jamadagni S. Fenugreek seed extract inhibit fat accumulation and ameliorates dyslipidemia in high fat diet-induced obese rats. BioMed Research International; 2014.
Handa T, Yamaguchi K, Sono Y, Yazawa K. Effects of fenugreek seed extract in obese mice fed a high-fat diet. Bioscience, Biotechnology and Biochemistry. 2005;69:1186-1188.
Council NR. Guide for the care and use of laboratory animals. National Academies Press; 2010.
-
Abstract View: 224 times
PDF Download: 184 times
