Screening of Enrofloxacin and Ciprofloxacin Residues in Chicken Meat by High-Performance Liquid Chromatography
Journal of Pharmaceutical Research International,
Page 64-69
DOI:
10.9734/jpri/2020/v32i2130753
Abstract
Enrofloxacin and Ciprofloxacin antibiotics are widely used in chicken production for prophylaxis and therapeutics purposes. Existence of these antibiotic residues in chicken meat can pose hazards to human health. The present study was aimed to assess the residue level of these antibiotics in chicken meat. Chicken meat samples (including muscle, liver, kidney and fat) from poultry farms and retail market were collected. High Performance Liquid Chromatography (HPLC) was used for screening of enrofloxacin and ciprofloxacin residues in chicken meat samples. The analysis revealed that 43.58% meat samples were positive for enrofloxacin and 38.71% for ciprofloxacin residues. Out of it, 45.17% samples were having concentration above the MRL for enrofloxacin and 50.28% for ciprofloxacin. So it can be concluded that the usage of these antimicrobial in chicken lead contamination of meat and it may cause resistance in consumers and seems to be a public health threat.
Keywords:
- Enrofloxacin
- ciprofloxacin
- residues
- Uttarakhand
How to Cite
Verma, M. K., Ahmad, A. H., Pant, D., Rawat, P., Sharma, S., & Arya, N. (2020). Screening of Enrofloxacin and Ciprofloxacin Residues in Chicken Meat by High-Performance Liquid Chromatography. Journal of Pharmaceutical Research International, 32(21), 64-69. https://doi.org/10.9734/jpri/2020/v32i2130753
References
López C, et al. Systemic and mammary gland disposition of enrofloxacin in healthy sheep following intramammary administration. BMC Vet. Res. 2015;11:88.
Trouchon T, Lefebvre S. A review of enrofloxacin for veterinary use. Open J. Vet. Med. 2016;06:40–58.
Yili Ding, Yuchang Pang, Chamakura VNS, Vara Prasad, Bingyun Wang. Formation of inclusion complex of enrofloxacin with 2-hydroxypropyl-β-cyclodextrin. Drug Delivery. 2020;27(1):334-343.
DOI: 10.1080/10717544.2020.1724210
Hooper DC. Emerging mechanisms of fluoroquinolone resistance. Emerging Infectious Diseases. 2001;7:337-341.
Lewis T, Cook J. Fluoroquinolones and tendinopathy: A guide for athletes and sports clinicians and a systematic review of the literature. Journal of Athletic Training. 2014;49(3):422-427.
CVMP. Public statement on the use of fuoroquinolones in food-producing animals in the European Union: Development of resistance and impact on human and animal health; 2007.
Dhary Alewy Al – Mashhadany, Nahla A. A. Abad Aljabar, Ahmed Mohammad Zaki, Vian Salim Mohammad. Detection of antibiotic residues among poultry meat in erbil city and impact of thermal processing on remnants. RJLBPCS. 2018;3(4):237.
Ramatla T, Ngoma L, Adetunji M, Mwanza M. Evaluation of antibiotic residues in raw meat using different analytical methods. Antibiotics. 2017;6(4):1–17.
Available:https://doi.org/10.3390/antibiotics6040034
Ewelina Patyra, Krzysztof Kwiatek. Determination of fluoroquinolones in animal feed by ion pair high-performance liquid chromatography with fluorescence detection. Analytical Letters; 2017.
DOI: 10.1080/00032719.2016.1249876
Varma R, Ahmad AH, Ahuja V, Gautam V, Pradeep K, Singh KP. Pharmacokinetic of enrofloxacin and its metabolite ciprofloxacin and their interaction with diclofenac sodium in cows. Indian Journal of Animal Sciences. 2006;76(1):6-9.
Shu-Chu Su, Mei-Hua Chang, Chin-Lin Chang, Pi-Chiou Chang, Shin-Shou Chou. Simultaneous determination of quinolones in livestock and marine products by high performance liquid chromatography. Journal of Food and Drug Analysis. 2003;11(2):114-127.
Babra Moyo, Nikita Tawanda Tavengwa. Modern extraction and cleanup methods of veterinary drug residues in food samples of animal origin; 2019.
DOI: 10.5772/intechopen.82656
Naeem M, Khan K, Rafiq S. Determination of residues of quinolones in poultry products by high performance liquid chromatography. Journal of Applied Sciences. 2006;6:373–379.
Qiao F, Sun H. Simultaneous extraction of enrofloxacin and ciprofloxacin from chicken tissue by molecularly imprinted matrix solid-phase dispersion. Journal of Pharmaceutical and Biomedical Analysis. 2010;53(3):795-798.
Seri HI. Introduction to veterinary drug residues: Hazards and risks. Veterinary drug residues in food derived from animals. The National Medicinal and Poisons Board, Khartoum, Sudan. 2013;1–7.
Godfred Darko, John Kenneth Mensah, Sylvester Samuel Dapaah, Judith Odei. Estimated dietary exposure to veterinary residues in chicken and eggs. International Journal of Food Contamination. 2015;2:16.
DOI: 10.1186/s40550-015-0022-2
Karin Hoelzer, Nora Wong, Joe Thomas, Kathy Talkington, Elizabeth Jungman, Allan Coukell. Antimicrobial drug use in food-producing animals and associated human health risks: What and how strong, is the evidence? BMC Veterinary Research. 2017;13:211.
Yorke JC, Froc P. Quantitation of nine quinolones in chicken tissues by high-performance liquid chromatography with fluorescence detection. J. Chromatography A. 2000;882:63-77.
Buket ER, Onurdağ FK, Demirhan B, Özgacar SO, Öktem AB, Abbasoğlu U. Screening of quinolone antibiotic residues in chicken meat and beef sold in the markets of Ankara, Turkey. Poultry Science. 2013;92(8):2212–2215.
Available:https://doi.org/10.3382/ps.2013-03072
Amro FH, Hassan MA, Mahmoud Ah. Spiramycin residues in chicken meat and giblets. Benha Veterinary Medical Journal. 2013;24(1):51-61.
Chaiba A, Filali FR, Chebaibi A. Investigation of antibiotics residues in poultry products in Meknes-Morocco. J. Advanc. Microbiol. 2017;2(1):1-8.
Shareef AM, James ZT, Yonis KM. Detection of antibiotic residues in stored poultry products. Iraqi Journal of Veterinary Science. 2009;23:45–48.
Bilal Aslam, Naheed Kousar, Ijaz Javed, Ahmad Raza, Asghar Ali, Tanweer Khaliq, Faqir Muhammad, Junaid Ali Khan. Determination of enrofloxacin residues in commercial broilers using high performance liquid chromatography. International Journal of Food Properties. 2016;19:2463–2470.
Zoltán Gregus. Casarett and Doull’s toxicology. The Basic Science of Poisons 8ed p-51. McGraw-Hill Education, New York; 2013.
Demetris AJ, Bellamy COC, Gandhi CR, Prost S, Nakanuma Y, Stolz DB. Functional immune anatomy of the liver as an allograft. American Journal of Transplantation. 2016;16:1653–1680.
Trouchon T, Lefebvre S. A review of enrofloxacin for veterinary use. Open J. Vet. Med. 2016;06:40–58.
Yili Ding, Yuchang Pang, Chamakura VNS, Vara Prasad, Bingyun Wang. Formation of inclusion complex of enrofloxacin with 2-hydroxypropyl-β-cyclodextrin. Drug Delivery. 2020;27(1):334-343.
DOI: 10.1080/10717544.2020.1724210
Hooper DC. Emerging mechanisms of fluoroquinolone resistance. Emerging Infectious Diseases. 2001;7:337-341.
Lewis T, Cook J. Fluoroquinolones and tendinopathy: A guide for athletes and sports clinicians and a systematic review of the literature. Journal of Athletic Training. 2014;49(3):422-427.
CVMP. Public statement on the use of fuoroquinolones in food-producing animals in the European Union: Development of resistance and impact on human and animal health; 2007.
Dhary Alewy Al – Mashhadany, Nahla A. A. Abad Aljabar, Ahmed Mohammad Zaki, Vian Salim Mohammad. Detection of antibiotic residues among poultry meat in erbil city and impact of thermal processing on remnants. RJLBPCS. 2018;3(4):237.
Ramatla T, Ngoma L, Adetunji M, Mwanza M. Evaluation of antibiotic residues in raw meat using different analytical methods. Antibiotics. 2017;6(4):1–17.
Available:https://doi.org/10.3390/antibiotics6040034
Ewelina Patyra, Krzysztof Kwiatek. Determination of fluoroquinolones in animal feed by ion pair high-performance liquid chromatography with fluorescence detection. Analytical Letters; 2017.
DOI: 10.1080/00032719.2016.1249876
Varma R, Ahmad AH, Ahuja V, Gautam V, Pradeep K, Singh KP. Pharmacokinetic of enrofloxacin and its metabolite ciprofloxacin and their interaction with diclofenac sodium in cows. Indian Journal of Animal Sciences. 2006;76(1):6-9.
Shu-Chu Su, Mei-Hua Chang, Chin-Lin Chang, Pi-Chiou Chang, Shin-Shou Chou. Simultaneous determination of quinolones in livestock and marine products by high performance liquid chromatography. Journal of Food and Drug Analysis. 2003;11(2):114-127.
Babra Moyo, Nikita Tawanda Tavengwa. Modern extraction and cleanup methods of veterinary drug residues in food samples of animal origin; 2019.
DOI: 10.5772/intechopen.82656
Naeem M, Khan K, Rafiq S. Determination of residues of quinolones in poultry products by high performance liquid chromatography. Journal of Applied Sciences. 2006;6:373–379.
Qiao F, Sun H. Simultaneous extraction of enrofloxacin and ciprofloxacin from chicken tissue by molecularly imprinted matrix solid-phase dispersion. Journal of Pharmaceutical and Biomedical Analysis. 2010;53(3):795-798.
Seri HI. Introduction to veterinary drug residues: Hazards and risks. Veterinary drug residues in food derived from animals. The National Medicinal and Poisons Board, Khartoum, Sudan. 2013;1–7.
Godfred Darko, John Kenneth Mensah, Sylvester Samuel Dapaah, Judith Odei. Estimated dietary exposure to veterinary residues in chicken and eggs. International Journal of Food Contamination. 2015;2:16.
DOI: 10.1186/s40550-015-0022-2
Karin Hoelzer, Nora Wong, Joe Thomas, Kathy Talkington, Elizabeth Jungman, Allan Coukell. Antimicrobial drug use in food-producing animals and associated human health risks: What and how strong, is the evidence? BMC Veterinary Research. 2017;13:211.
Yorke JC, Froc P. Quantitation of nine quinolones in chicken tissues by high-performance liquid chromatography with fluorescence detection. J. Chromatography A. 2000;882:63-77.
Buket ER, Onurdağ FK, Demirhan B, Özgacar SO, Öktem AB, Abbasoğlu U. Screening of quinolone antibiotic residues in chicken meat and beef sold in the markets of Ankara, Turkey. Poultry Science. 2013;92(8):2212–2215.
Available:https://doi.org/10.3382/ps.2013-03072
Amro FH, Hassan MA, Mahmoud Ah. Spiramycin residues in chicken meat and giblets. Benha Veterinary Medical Journal. 2013;24(1):51-61.
Chaiba A, Filali FR, Chebaibi A. Investigation of antibiotics residues in poultry products in Meknes-Morocco. J. Advanc. Microbiol. 2017;2(1):1-8.
Shareef AM, James ZT, Yonis KM. Detection of antibiotic residues in stored poultry products. Iraqi Journal of Veterinary Science. 2009;23:45–48.
Bilal Aslam, Naheed Kousar, Ijaz Javed, Ahmad Raza, Asghar Ali, Tanweer Khaliq, Faqir Muhammad, Junaid Ali Khan. Determination of enrofloxacin residues in commercial broilers using high performance liquid chromatography. International Journal of Food Properties. 2016;19:2463–2470.
Zoltán Gregus. Casarett and Doull’s toxicology. The Basic Science of Poisons 8ed p-51. McGraw-Hill Education, New York; 2013.
Demetris AJ, Bellamy COC, Gandhi CR, Prost S, Nakanuma Y, Stolz DB. Functional immune anatomy of the liver as an allograft. American Journal of Transplantation. 2016;16:1653–1680.
-
Abstract View: 320 times
PDF Download: 260 times
Download Statistics
Downloads
Download data is not yet available.
