Evaluation of Calcium and Alkaline Phosphatase in the Salivary Samples of Premenopausal and Postmenopausal Women
Journal of Pharmaceutical Research International,
The quality of life for women after menopause is one of the key health issues today. Rapid bone loss occurs in post-menopausal women due to hormonal factors that lead to an increased risk of fractures. The aim of the study is to evaluate the calcium and Alkaline phosphate levels (ALP) in the salivary samples of premenopausal and postmenopausal women. The study was conducted with a sample size of 30, with 15 samples from premenopausal women belonging to the age group of 40-46 years and 15 samples from postmenopausal women aged more than 50 years. Calcium and alkaline phosphatase levels were estimated using the commercial kits available which were Spinreact and Accucare alkaline phosphatase respectively. The statistical analysis was done using independent t-test. Saliva was stimulated and biochemical analysis of these salivary samples was done to evaluate the calcium and alkaline phosphatase. Mean salivary calcium in premenopausal women was found to be 7.37±1.141 mg/dl and in postmenopausal women it was found to be 5.19±1.141mg/dl,P=0.000(p<0.05). Mean salivary ALP levels in premenopausal women was found to be 10.81±3.295 mg/dl and in postmenopausal women was found to be 33.58±3.295 mg/dl, P=0.001(p <0.05), hence statistically significant. Within the limitations of the study, it was found that calcium were found to be significantly more in premenopausal women when compared to postmenopausal women, while alkaline phosphatase levels were increased in postmenopausal women when compared to premenopausal women. Hence, salivary calcium and ALP levels can be used as biomarkers for diagnosis of bone diseases as it is a non-invasive technique.
- Alkaline phosphatase
How to Cite
Reddy S, Karthikeyan R, Herald Justin Sherlin, Anuja N, Pratibha R, Priya P, et al. Oral signs and salivary parameters as indicators of possible osteoporosis and osteopenia in postmenopausal women - A study of 45 subjects. Brazilian Journal of Oral Sciences. 2008;7(24):1502–6.
Rabiei M, Masooleh IS, Leyli EK, Nikoukar LR. Salivary calcium concentration as a screening tool for postmenopausal osteoporosis. Int J Rheum Dis. 2013;16(2): 198–202.
Frutos R, Rodríguez S, Miralles-Jorda L, Machuca G. Oral manifestations and dental treatment in menopause. Med Oral. 2002;7(1):26–30, 31–5.
Nederfors T. Xerostomia and hyposalivation. Adv Dent Res. 2000;14: 48–56.
Agha-Hosseini F, Mirzaii-Dizgah I, Moghaddam PP, Akrad ZT. Stimulated whole salivary flow rate and composition in menopausal women with oral dryness feeling. Oral Dis. 2007;13(3):320–3.
Ben Aryeh H, Gottlieb I, Ish-Shalom S, David A, Szargel H, Laufer D. Oral complaints related to menopause. Maturitas. 1996;24(3):185–9.
Tarkkila L, Linna M, Tiitinen A, Lindqvist C, Meurman JH. Oral symptoms at menopause—the role of hormone replacement therapy. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology. 2001 1;92(3):276–80.
Borhan MK, Esfahani M, Jahani HH. Evaluation of unstimulated salivary flow rate and oral symptoms in menopausal women; 2007.
Sevón L, Laine MA, Karjalainen S, Doroguinskaia A, Helenius H, Kiss E, et al. Effect of age on flow-rate, protein and electrolyte composition of stimulated whole saliva in healthy, non-smoking women. Open Dent J. 2008;2:89–92.
Agha-Hosseini F, Mirzaii-Dizgah I, Mansourian A, Zabihi-Akhtechi G. Serum and stimulated whole saliva parathyroid hormone in menopausal women with oral dry feeling. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009;107(6):806–10.
Delmas PD, Eastell R, Garnero P, Seibel MJ, Stepan J. The use of biochemical markers of bone turnover in osteoporosis [Internet]. Osteoporosis International. 2000;11:S2–17.
Subramaniam N, Muthukrishnan A. Oral mucositis and microbial colonization in oral cancer patients undergoing radiotherapy and chemotherapy: A prospective analysis in a tertiary care dental hospital. J Investig Clin Dent. 2019;10(4):e12454.
Vadivel JK, Govindarajan M, Somasundaram E, Muthukrishnan A. Mast cell expression in oral lichen planus: A systematic review. J Investig Clin Dent. 2019;10(4):e12457.
Patil SR, Maragathavalli G, Ramesh DNSV, Vargheese S, Al-Zoubi IA, Alam MK. Assessment of maximum bite force in oral submucous fibrosis patients: A Preliminary Study. Pesqui Bras Odontopediatria Clin Integr. 2020;20:482.
Patil SR, Maragathavalli G, Araki K, Al-Zoubi IA, Sghaireen MG, Gudipaneni RK, et al. Three-rooted mandibular first molars in a Saudi Arabian population: A CBCT study. Pesqui Bras Odontopediatria Clin Integr. 2018;18(1):e4133.
Patil SR, Yadav N, Al-Zoubi IA, Maragathavalli G, Sghaireen MG, Gudipaneni RK, et al. Comparative study of the efficacy of newer antioxitands lycopene and oxitard in the treatment of oral submucous fibrosis. Pesqui Bras Odontopediatria Clin Integr. 2018;18(1):1–7.
Acharya D, Gautam S, Neupane N, Kaphle HP, Singh JK. Health problems of women above forty years of age in rupandehi district of Nepal. Int J Health Sci Res. 2013;3(3):29–36.
Nelson HD, Haney E, Humphrey L, Others. Management of menopause-related symptoms (Agency of Healthcare Research and Quality, Rockville, MD) Evidence report. Technology Assessment. 2005;120.
Ishikawa K, Nagai T, Sakamoto K, Ohara K, Eguro T, Ito H, et al. High bone turnover elevates the risk of denosumab-induced hypocalcemia in women with postmenopausal osteoporosis. Ther Clin Risk Manag. 2016;12:1831–40.
Streckfus CF, Bigler LR. Saliva as a diagnostic fluid. Oral Dis. 2002;8(2):69–76.
Ng PYB, Donley M, Hausmann E, Hutson AD, Rossomando EF, Scannapieco FA. Candidate salivary biomarkers associated with alveolar bone loss: Cross-sectional and in vitro studies. FEMS Immunol Med Microbiol. 2007;49(2):252–60.
Khan YS, Srivastava A, Shahni R, Srivastava V, Yadav S, Husain S. Comparative analysis of salivary alkaline phosphatase in pre and post-menopausal women with and without periodontitis. University Journal of Dental Sciences. 2019;5(2):23–6.
Matsuura T, Tokutomi K, Sasaki M, Katafuchi M, Mizumachi E, Sato H. Distinct characteristics of mandibular bone collagen relative to long bone collagen: relevance to clinical dentistry. Biomed Res Int. 2014;2014:769414.
Suresh M, Naidu DM. Influence of years since menopause on bone mineral metabolism in South Indian women. Indian J Med Sci. 2006;60(5):190–8.
Ashuma S, Shashi S, Sachdeva S. Biochemical Markers of bone turnover: diagnostic and therapeutic principles. Osteoporos Int. 2005;3(2):305–11.
Massé PG, Dosy J, Jougleux J-L, Caissie M, Howell DS. Bone Mineral Density and Metabolism at an Early Stage of Menopause When Estrogen and Calcium Supplement Are Not Used and without the Interference of Major Confounding Variables [Internet]. Journal of the American College of Nutrition. 2005;24: 354–60.
Bhattarai T, Bhattacharya K, Chaudhuri P, Sengupta P. Correlation of common biochemical markers for bone turnover, serum calcium, and alkaline phosphatase in post-menopausal women. Malays J Med Sci. 2014;21(1):58–61.
Ramesh A, Bhandary R, Thomas B, D'Souza SR, Kumari S. Alkaline phosphatase-a diagnostic marker of periodontitis in postmenopausal women-a biochemical study. Nitte University Journal of Health Science. 2013;3(4):71.
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