Comparative Evaluation of Inherent Antimicrobial Properties and Bacterial Surface Adherence between Copper and Stainless Steel Suction Tube
Journal of Pharmaceutical Research International,
Page 149-156
DOI:
10.9734/jpri/2020/v32i1930721
Abstract
Aim of the present study is comparative evaluation of the inherent antimicrobial properties of the copper and stainless steel suction tube also evaluate bacterial adherence and reduction with time within the suction tube. Present study was performed in an in vitro setting in Saveetha dental college and hospital. Both the metals suction tubes were cut into equal lengths of 2.3 inches and dipped in a sterile container for 5 minutes which had bacterial suspension of Streptococcus Mutans. Each group included three samples of copper and stainless steel suction tubes. Control group was taken out at 0 mins post initial dipping period de-coated in a sterile container and immediately cultured on Brain heart infusion agar, followed by group 1 at 15 minutes group 2 at 60 minutes and group 3 at 180 minutes respectively. Bacterial colonies were counted after overnight incubation. Copper suction tubes demonstrated less initial adhesion of bacterial colonies than that of stainless steel suction tubes. Percentage reduction in bacterial colonies was observed more in stainless steel suction tubes with respect to time. Spite of high initial adhesion stainless steel suction tube demonstrated higher degree of percentage reduction in bacterial colonies with time hence proven better for antimicrobial properties than copper suction tube.
Keywords:
- Antimicrobial property
- cross infection
- copper
- contact killing
- stainless steel
- suction tube
- S. mutans
How to Cite
Ketkar, G. N., Malaiappan, S., & N., M. (2020). Comparative Evaluation of Inherent Antimicrobial Properties and Bacterial Surface Adherence between Copper and Stainless Steel Suction Tube. Journal of Pharmaceutical Research International, 32(19), 149-156. https://doi.org/10.9734/jpri/2020/v32i1930721
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Boyle MA, O’Donnell MJ, Russell RJ, et al. Overcoming the problem of residual microbial contamination in dental suction units left by conventional disinfection using novel single component suction handpieces in combination with automated flood disinfection. J Dent. 2015;43:1268–1279.
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Sifri CD, Burke GH, Enfield KB. Reduced health care-associated infections in an acute care community hospital using a combination of self-disinfecting copper-impregnated composite hard surfaces and linens. Am J Infect Control. 2016;44:1565–1571.
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Accessed 4 July 2020.
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Ravi S, Malaiappan S, Varghese S, et al. Additive effect of plasma rich in growth factors with guided tissue regeneration in treatment of intrabony defects in patients with chronic periodontitis:A split-mouth randomized controlled clinical trial. J Periodontol. 2017;88:839–845.
Kavarthapu A, Malaiappan S. Comparative evaluation of demineralized bone matrix and type II collagen membrane versus eggshell powder as a graft material and membrane in rat model. Indian J Dent Res. 2019;30:877–880.
Murthykumar K, Arjunkumar R, Jayaseelan VP. Association of vitamin D receptor gene polymorphism (rs10735810) and chronic periodontitis. J Investig Clin Dent. 2019; 10:12440.
Ramesh A, Vellayappan R, Ravi S, et al. Esthetic lip repositioning: A cosmetic approach for correction of gummy smile - A case series. J Indian Soc Periodontol. 2019;23:290–294.
Ramesh A, Varghese S, Jayakumar ND, et al. Comparative estimation of sulfiredoxin levels between chronic periodontitis and healthy patients - A case-control study. J Periodontol. 2018;89:1241–1248.
Kavarthapu A, Thamaraiselvan M. Assessing the variation in course and position of inferior alveolar nerve among south Indian population: A cone beam computed tomographic study. Indian J Dent Res. 2018;29:405–409.
Ramesh A, Ravi S, Kaarthikeyan G. Comprehensive rehabilitation using dental implants in generalized aggressive periodontitis. J Indian Soc Periodontol. 2017;21:160–163.
Jain M, Nazar N. Comparative evaluation of the efficacy of intraligamentary and supraperiosteal injections in the extraction of maxillary teeth: A randomized controlled clinical trial. J Contemp Dent Pract. 2018; 19:1117–1121.
Vijayashree Priyadharsini J. In silico validation of the non-antibiotic drugs acetaminophen and ibuprofen as antibacterial agents against red complex pathogens. J Periodontol. 2019;90:1441–1448.
Amachawadi RG, Scott HM, Vinasco J, et al. Effects of in-feed copper, chlortetracycline, and tylosin on the prevalence of transferable copper resistance gene, tcrB, among fecal enterococci of weaned piglets. Foodborne Pathog Dis. 2015;12:670–678.
Solioz M. Copper and Bacteria: Evolution, homeostasis and toxicity. Springer; 2018.
Avaiable:https://play.google.com/store/books/details?id=5LZhDwAAQBAJ
Elguindi J, Wagner J, Rensing C. Genes involved in copper resistance influence survival of Pseudomonas aeruginosa on copper surfaces. J Appl Microbiol. 2009; 106:1448–1455.
Grass G, Rensing C, Solioz M. Metallic copper as an antimicrobial surface. Appl Environ Microbiol. 2011;77:1541–1547.
Santo CE, Quaranta D, Grass G. Antimicrobial metallic copper surfaces kill Staphylococcus haemolyticusvia membrane damage. Microbiology Open. 2012;1:46–52.
Santo CE, Taudte N, Nies DH, et al. Contribution of copper ion resistance to survival of Escherichia coli on metallic copper surfaces. Applied and Environmental Microbiology. 2008;74:977–986.
Nandakumar R, Santo CE, Madayiputhiya N, et al. Quantitative proteomic profiling of the Escherichia coli response to metallic copper surfaces. BioMetals. 2011;24:429–444.
Weber DJ, Otter JA, Rutala WA. Can Copper-Coated Surfaces Prevent Healthcare-Associated Infections? Infection control and hospital epidemiology: The official journal of the Society of Hospital Epidemiologists of America. 2017;38:772–776.
Vincent M, Duval RE, Hartemann P, et al. Contact killing and antimicrobial properties of copper. Journal of Applied Microbiology. 2018;124:1032–1046.
Montero DA, Arellano C, Pardo M, et al. Antimicrobial properties of a novel copper-based composite coating with potential for use in healthcare facilities. Antimicrob Resist Infect Control. 2019;8:3.
Allegranzi B, Bagheri Nejad S, Combescure C, et al. Burden of endemic health-care-associated infection in developing countries: Systematic review and meta-analysis. Lancet 2011;377:228–241.
Barahona N, Rodriguez M, De Moya Y. Importancia de la vigilancia epidemiológica en el control de las infecciones asociadas a la atención en salud. Biociencias. 2019; 14:79–96.
Barriga J, Cerda J, Abarca K, et al. Infecciones asociadas a la atención en salud (IAAS) en pacientes pediátricos post-operados de cardiopatías congénitas. Revista chilena de; 2014.
Available:https://scielo.conicyt.cl/scielo.php?pid=S0716-10182014000100002&script =sci_arttext&tlng=e
Brenner P, Nercelles P, Pohlenz M, et al. Costo de las infecciones intrahospitalarias en hospitales chilenos de alta y mediana complejidad. Revista chilena de infectología. 2003;20:285–290.
Attaway HH 3rd, Fairey S, Steed LL, et al. Intrinsic bacterial burden associated with intensive care unit hospital beds: Effects of disinfection on population recovery and mitigation of potential infection risk. Am J Infect Control. 2012;40:907–912.
Kramer A, Schwebke I, Kampf G. How long do nosocomial pathogens persist on inanimate surfaces? A systematic review. BMC Infect Dis. 2006;6:130.
Rutala WA, Weber DJ. Guideline for disinfection and sterilization in healthcare facilities; 2008.
Available:https://stacks.cdc.gov/view/cdc/47378
Prado V, Vidal R, Durán C. Aplicación de la capacidad bactericida del cobre en la práctica médica. Revista médica de Chile. 2012;140:1325–1332.
Boyle MA, O’Donnell MJ, Russell RJ, et al. Overcoming the problem of residual microbial contamination in dental suction units left by conventional disinfection using novel single component suction handpieces in combination with automated flood disinfection. J Dent. 2015;43:1268–1279.
von Dessauer B, Navarrete MS, Benadof D, et al. Potential effectiveness of copper surfaces in reducing health care–associated infection rates in a pediatric intensive and intermediate care unit: A nonrandomized controlled trial. Am J Infect Control. 2016;44:133–139.
Sifri CD, Burke GH, Enfield KB. Reduced health care-associated infections in an acute care community hospital using a combination of self-disinfecting copper-impregnated composite hard surfaces and linens. Am J Infect Control. 2016;44:1565–1571.
Marais F, Mehtar S, Chalkley L. Antimicrobial efficacy of copper touch surfaces in reducing environmental bioburden in a South African community healthcare facility. J Hosp Infect. 2010;74: 80–82.
Kalsi A, Balani N. Cleaning, disinfection and sterilisation. Physics for the Anaesthetic Viva.103–106.
Kenley RS. Devices, systems, and methods for cleaning, disinfecting, rinsing, and priming blood separation devices and associated fluid lines. 2013;8562908.
Available:https://patentimages.storage.googleapis.com/a2/35/50/ba25c0b6775fc7/US8562908.pdf
Accessed 4 July 2020.
Robertshaw RG. Automatic cleaning and disinfection of suction bottles. Journal of Hospital Infection. 1982;3:299–302.
De R. Cleaning, Sterilisation and Disinfection. Diagnostic Microbiology (For DMLT Students). 2007;12–12.
Ezhilarasan D, Apoorva VS, Ashok Vardhan N. Syzygium cumini extract induced reactive oxygen species-mediated apoptosis in human oral squamous carcinoma cells. J Oral Pathol Med. 2019; 48:115–121.
Gajendran PL, Parthasarathy H, Tadepalli A. Comparative evaluation of cathepsin K levels in gingival crevicular fluid among smoking and nonsmoking patients with chronic periodontitis. Indian J Dent Res. 2018;29:588–593.
Kaarthikeyan G, Jayakumar ND, Sivakumar D. Comparative evaluation of bone formation between prf and blood clot alone as the sole sinus-filling material in maxillary sinus augmentation with the implant as a tent pole: A Randomized Split-Mouth Study. J Long Term Eff Med Implants. 2019;29:105–111.
Arjunkumar R. Nanomaterials for the Management of Periodontal Diseases. In: Chaughule RS (Ed) Dental Applications of Nanotechnology. Cham: Springer International Publishing. 203–215.
Ravi S, Malaiappan S, Varghese S, et al. Additive effect of plasma rich in growth factors with guided tissue regeneration in treatment of intrabony defects in patients with chronic periodontitis:A split-mouth randomized controlled clinical trial. J Periodontol. 2017;88:839–845.
Kavarthapu A, Malaiappan S. Comparative evaluation of demineralized bone matrix and type II collagen membrane versus eggshell powder as a graft material and membrane in rat model. Indian J Dent Res. 2019;30:877–880.
Murthykumar K, Arjunkumar R, Jayaseelan VP. Association of vitamin D receptor gene polymorphism (rs10735810) and chronic periodontitis. J Investig Clin Dent. 2019; 10:12440.
Ramesh A, Vellayappan R, Ravi S, et al. Esthetic lip repositioning: A cosmetic approach for correction of gummy smile - A case series. J Indian Soc Periodontol. 2019;23:290–294.
Ramesh A, Varghese S, Jayakumar ND, et al. Comparative estimation of sulfiredoxin levels between chronic periodontitis and healthy patients - A case-control study. J Periodontol. 2018;89:1241–1248.
Kavarthapu A, Thamaraiselvan M. Assessing the variation in course and position of inferior alveolar nerve among south Indian population: A cone beam computed tomographic study. Indian J Dent Res. 2018;29:405–409.
Ramesh A, Ravi S, Kaarthikeyan G. Comprehensive rehabilitation using dental implants in generalized aggressive periodontitis. J Indian Soc Periodontol. 2017;21:160–163.
Jain M, Nazar N. Comparative evaluation of the efficacy of intraligamentary and supraperiosteal injections in the extraction of maxillary teeth: A randomized controlled clinical trial. J Contemp Dent Pract. 2018; 19:1117–1121.
Vijayashree Priyadharsini J. In silico validation of the non-antibiotic drugs acetaminophen and ibuprofen as antibacterial agents against red complex pathogens. J Periodontol. 2019;90:1441–1448.
Amachawadi RG, Scott HM, Vinasco J, et al. Effects of in-feed copper, chlortetracycline, and tylosin on the prevalence of transferable copper resistance gene, tcrB, among fecal enterococci of weaned piglets. Foodborne Pathog Dis. 2015;12:670–678.
Solioz M. Copper and Bacteria: Evolution, homeostasis and toxicity. Springer; 2018.
Avaiable:https://play.google.com/store/books/details?id=5LZhDwAAQBAJ
Elguindi J, Wagner J, Rensing C. Genes involved in copper resistance influence survival of Pseudomonas aeruginosa on copper surfaces. J Appl Microbiol. 2009; 106:1448–1455.
Grass G, Rensing C, Solioz M. Metallic copper as an antimicrobial surface. Appl Environ Microbiol. 2011;77:1541–1547.
Santo CE, Quaranta D, Grass G. Antimicrobial metallic copper surfaces kill Staphylococcus haemolyticusvia membrane damage. Microbiology Open. 2012;1:46–52.
Santo CE, Taudte N, Nies DH, et al. Contribution of copper ion resistance to survival of Escherichia coli on metallic copper surfaces. Applied and Environmental Microbiology. 2008;74:977–986.
Nandakumar R, Santo CE, Madayiputhiya N, et al. Quantitative proteomic profiling of the Escherichia coli response to metallic copper surfaces. BioMetals. 2011;24:429–444.
Weber DJ, Otter JA, Rutala WA. Can Copper-Coated Surfaces Prevent Healthcare-Associated Infections? Infection control and hospital epidemiology: The official journal of the Society of Hospital Epidemiologists of America. 2017;38:772–776.
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