Assessment of Bacterial Micro Colonies Present in Tissue Samples Obtained Using Ozonated Water as an Irrigating Agent from Patients with Periodontitis
Journal of Pharmaceutical Research International,
Periodontal diseases are chronic inflammatory diseases that are constantly associated with microorganism, conventionally scaling and root planing followed by flap surgical procedure is done to treat chronic periodontitis condition, irrigating agents are used during both sub gingival scaling and root planing procedure and in flap surgical procedure. Saline and distilled water are the commonly used irrigants and they non specifically reduce the bacterial count, Ozonised water is also used as an irrigating agent. Though it’s effect on plaque is known to be effective its effect on tissues is still questionable. The study is conducted to assess the number bacterial micro colonies formed in gingival tissues after irrigating with ozonated water and comparing it with distilled water and saline water which are the commonly used irrigating agents.
The aim of the study is to assess the bacterial micro colonies formed in gingival tissues after irrigating with ozonated water and comparing it with distilled water and saline water which are the commonly used irrigating agents.
A randomized, split mouth study was performed. A total of 10 patients suffering from chronic generalised periodontitis were recruited into the study; 20 gingival tissue samples were obtained from the patients at the time of surgery. Both the patient and the clinician were blinded regarding the type of the irrigating agent used. The plaque samples were transferred into carrier medium and were cultured for 24 hours; 24 h after culture, the samples were collected and total colony forming units were calculated. On counting the number of colony forming units formed, it was found that the amount of colonies formed from the samples treated with ozone showed mixed results, wherein it showed both increase and decrease in colony counts.
- bacterial count
- ozone therapy
- irrigating agent
- distilled water
- ozonated water
How to Cite
Saini R, Marawar PP, Shete S, et al. Periodontitis, A true infection. J Glob Infect Dis. 2009;1:149–150.
Socransky SS, Haffajee AD. The bacterial etiology of destructive periodontal disease: Current concepts. Journal of Periodontology. 1992;63:322–331.
Moore WEC, Moore LVH. The bacteria of periodontal diseases. Periodontology 2000. 1994;5:66–77.
Lynch E. Ozone: The revolution in dentistry; 2004.
Burns DT, Thorburn Burns D. Early problems in the analysis and the determination of ozone. Fresenius’ Journal of Analytical Chemistry. 1997;357:178–183.
Nogales CG, Ferrari PH, Kantorovich EO, et al. Ozone therapy in medicine and dentistry. J Contemp Dent Pract. 2008;9: 75–84.
Holmes J. Clinical reversal of root caries using ozone, double-blind, randomised, controlled 18-month trial. Gerodontology. 2003;20:106–114.
Edmondo F. Apparatus for the production and use of ozone in therapeutics. 1936;2054367.
Accessed 4 July 2020.
Makkar S, Makkar M. Ozone - treating dental infections. 2011;2:256–259.
Srikanth A, Sathish M, Sri Harsha AV. Application of ozone in the treatment of periodontal disease. J Pharm Bioallied Sci. 2013;5:S89–94.
Seidler V, Linetskiy I, Hubálková H, et al. Ozone and its usage in general medicine and dentistry. A review article. Prague Med Rep. 2008;109:5–13.
Deepa D, Gupta S. Applications of ozone therapy in dentistry. Journal of Oral Research and Review. 2016;8:86.
Ezhilarasan D, Apoorva VS, Ashok Vardhan N. Syzygium cumini extract induced reactive oxygen species-mediated apoptosis in human oral squamous carcinoma cells. J Oral Pathol Med. 2019; 48:115–121.
Kaarthikeyan G, Jayakumar ND, Sivakumar D. Comparative evaluation of bone formation between PRF and blood clot alone as the sole sinus-filling material in maxillary sinus augmentation with the implant as a tent pole: A Randomized Split-Mouth Study. J Long Term Eff Med Implants. 2019;29:105–111.
Arjunkumar R. Nanomaterials for the Management of Periodontal Diseases. In: Chaughule RS (ed). Dental Applications of Nanotechnology. Cham: Springer International Publishing. 2018;203–215.
Ravi S, Malaiappan S, Varghese S, et al. Additive effect of plasma rich in growth factors with guided tissue regeneration in treatment of intrabony defects in patients with chronic periodontitis: A split-mouth randomized controlled clinical trial. J Periodontol. 2017;88:839–845.
Kavarthapu A, Malaiappan S. Comparative evaluation of demineralized bone matrix and type II collagen membrane versus eggshell powder as a graft material and membrane in rat model. Indian J Dent Res. 2019;30:877–880.
Murthykumar K, Arjunkumar R, Jayaseelan VP. Association of vitamin D receptor gene polymorphism (rs10735810) and chronic periodontitis. J Investig Clin Dent. 2019; 10:e12440.
Ramesh A, Vellayappan R, Ravi S, et al. Esthetic lip repositioning: A cosmetic approach for correction of gummy smile - A case series. J Indian Soc Periodontol. 2019;23:290–294.
Ramesh A, Varghese S, Jayakumar ND, et al. Comparative estimation of sulfiredoxin levels between chronic periodontitis and healthy patients - A case-control study. J Periodontol. 2018;89:1241–1248.
Kavarthapu A, Thamaraiselvan M. Assessing the variation in course and position of inferior alveolar nerve among south Indian population: A cone beam computed tomographic study. Indian J Dent Res. 2018;29:405–409.
Ramesh A, Ravi S, Kaarthikeyan G. Comprehensive rehabilitation using dental implants in generalized aggressive periodontitis. J Indian Soc Periodontol 2017;21:160–163.
Jain M, Nazar N. Comparative evaluation of the efficacy of intraligamentary and supraperiosteal injections in the extraction of maxillary teeth: A randomized controlled clinical trial. J Contemp Dent Pract. 2018; 19:1117–1121.
Vijayashree Priyadharsini J. In silico validation of the non-antibiotic drugs acetaminophen and ibuprofen as antibacterial agents against red complex pathogens. J Periodontol. 2019;90:1441–1448.
Ramamurthy J, Mg V. Comparison of effect of hiora mouthwash versus chlorhexidine mouthwash in gingivitis patients: a clinical trial. Asian J Pharm Clin Res. 2018;11:84–88.
Skurska A, Pietruska MD, Paniczko-Drężek A, et al. Evaluation of the influence of ozonotherapy on the clinical parameters and MMP levels in patients with chronic and aggressive periodontitis. Advances in Medical Sciences. 2010;55:297–307.
Vasthavi C, Babu HM, Rangaraju VM, et al. Evaluation of ozone as an adjunct to scaling and root planing in the treatment of chronic periodontitis: A randomized clinico-microbial study. J Indian Soc Periodontol 2020;24:42–46.
Habashneh RA, Al Habashneh R, Alsalman W, et al. Ozone as an adjunct to conventional nonsurgical therapy in chronic periodontitis: A randomized controlled clinical trial. Journal of Periodontal Research. 2015;50:37–43.
Niveda R, Malaiappan S. Comparison of the amount of bacterial microcolonies formed on using distilled water and ozonated water as an irrigating agent; 2019.
Accessed 6 July 2020.
Ramzy MI, Gomaa HE, Mostafa MI, et al. Management of aggressive periodontitis using ozonized water. 2005;6.
Accessed 4 July 2020.
Nagayoshi M, Fukuizumi T, Kitamura C, et al. Efficacy of ozone on survival and permeability of oral microorganisms. Oral Microbiology and Immunology. 2004;19: 240–246.
Laxman V, Kshitish D. The use of ozonated water and 0.2% chlorhexidine in the treatment of periodontitis patients: A clinical and microbiologic study. Indian Journal of Dental Research. 2010;21:341.
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