Evaluation of Antioxidant and Cytotoxic Effect of Selenium Nanoparticles Synthesised Using Capparis decidua
Journal of Pharmaceutical Research International,
Page 60-66
DOI:
10.9734/jpri/2020/v32i1930709
Abstract
Background: Among the nanoparticles, selenium nanoparticles (SeNP) are one of the most extensively studied as Selenium has zero oxidation state, non toxic and biologically inert material. This is the reason why Selenium is considered as a major nanoparticulate. In this study SeNPs were extracted from the fruit of Capparis decidua which is a xerophytic small herb.
Aim: The aim of the present study is to evaluate the cytotoxic effect and antioxidant capacity of selenium nanoparticles.
Materials and Methods: In this study SeNPs were extracted from the fruit of Capparis decidua which is a xerophytic small herb. The cytotoxic effect of SeNPs was evaluated using Brine Shrimp assay and the antioxidant activity was determined using DPPH assay considering ascorbic acid as the standard.
Results: From the study of this assay the shrimps introduced into the well were almost alive in different concentrations and this indicates that there is no cytotoxicity in selenium nanoparticles. The percentage inhibition of Selenium nanoparticles in 10 µl was 15.4 0.1, 20 µl was 38.36 0.15, 30 µl was 45.3 0.1, 40 µl was 59.6 0.15 and 50 µl was 65.6 0.1. It can be inferred that percentage inhibition increases with increase in concentration but it was less when compared to the percentage inhibition of the standard.
Conclusion: The selenium nanoparticles extracted from Capparis decidua do not have any cytotoxic effect on shrimps. The SeNPs possessed significant antioxidant activity in increasing concentrations compared to the standard used. Thus SeNPs are biologically useful and can be used as eco-friendly, cost effective and efficient biomedical agents and therapeutics.
Keywords:
- Selenium nanoparticles
- Capparis decidua
- cytotoxicity
- antioxidant
- non-toxic
- brine shrimps
- DPPH radical
How to Cite
Lakshme, P. S. T., S, P., Jeevitha, M., & Rajeshkumar, S. (2020). Evaluation of Antioxidant and Cytotoxic Effect of Selenium Nanoparticles Synthesised Using Capparis decidua. Journal of Pharmaceutical Research International, 32(19), 60-66. https://doi.org/10.9734/jpri/2020/v32i1930709
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Tayebee R, Pejhan A, Ramshini H, et al. Equisetum arvense as an abundant source of silica nanoparticles. SiO2 /H3 PW12 O40 nanohybrid material as an efficient and environmental benign catalyst in the synthesis of 2-amino-4H-chromenes under solvent-free conditions. Applied Organometallic Chemistry. 2018;32:e3924.
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Gunti L, Dass RS, Kalagatur NK. Phytofabrication of selenium nanoparticles from Emblica officinalis fruit extract and exploring its biopotential applications: Antioxidant, antimicrobial and bio-compatibility. Frontiers in Microbiology; 10. Epub ahead of print; 2019.
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Zia-Ul-Haq M, Ćavar S, Qayum M, et al. Compositional studies: Antioxidant and antidiabetic activities of Capparis decidua (Forsk.) edgew. International Journal of Molecular Sciences. 2011;12:8846–8861.
Boroumand S, Safari M, Shaabani E, et al. Selenium nanoparticles: Synthesis, characterization and study of their cytotoxicity, antioxidant and antibacterial activity. Materials Research Express. 2019;6:0850d8.
Liu W, Li X, Wong Y-S, et al. Selenium Nanoparticles as a carrier Of 5-Fluorouracil to achieve anticancer synergism. ACS Nano. 2012;6:6578–6591.
McKenzie RC. Selenium, ultraviolet radiation and the skin. Clinical and Experimental Dermatology. 2000;25:631–636.
Qin S, Huang B, Ma J, et al. effects of selenium-chitosan on blood selenium concentration, antioxidation status, and cellular and humoral immunity in mice. Biological Trace Element Research. 2015;165:145–152.
Wang H, Zhang J, Yu H. Elemental selenium at nano size possesses lower toxicity without compromising the fundamental effect on selenoenzymes: Comparison with selenomethionine in mice. Free Radical Biology and Medicine. 2007;42:1524–1533.
Torres SK, Campos VL, León CG, et al. Biosynthesis of selenium nanoparticles by Pantoea agglomerans and their antioxidant activity. Journal of Nanoparticle Research; 14. Epub ahead of print; 2012.
DOI: 10.1007/s11051-012-1236-3
Wang W, Mai K, Zhang W, et al. Dietary selenium requirement and its toxicity in juvenile abalone Haliotis discus hannai Ino. Aquaculture. 2012;330-333:42–46.
Zhang J, Wang H, Yan X, et al. Comparison of short-term toxicity between Nano-Se and selenite in mice. Life Sciences. 2005;76:1099–1109.
Chen H, Shin D-W, Nam J-G, et al. Selenium nanowires and nanotubes synthesized via a facile template-free solution method. Materials Research Bulletin. 2010;45:699–704.
Kaur G, Iqbal M, Bakshi MS. Biomineralization of fine selenium crystalline rods and amorphous spheres. The Journal of Physical Chemistry C. 2009;113:13670–13676.
Zhang Y, Wang J, Zhang L. Creation of highly stable selenium nanoparticles capped with hyperbranched polysaccharide in water. Langmuir. 2010;26:17617–17623.
Andersen MH, Schrama D, Thor Straten P, et al. Cytotoxic T cells. J Invest Dermatol. 2006;126:32–41.
Jingwen B, Yaochen L, Guojun Z. Cell cycle regulation and anticancer drug discovery. Cancer Biology & Medicine. 2017;14:348.
Abner EL, Schmitt FA, Mendiondo MS, et al. Vitamin E and all-cause mortality: A meta-analysis. Current Aging Sciencee. 2011;4:158–170.
Shekelle PG, Morton SC, Jungvig LK, et al. Effect of supplemental vitamin E for the prevention and treatment of cardiovascular disease. Journal of General Internal Medicine. 2004;19:380–389.
Duarte TL, Lunec J. Review part of the series: From dietary antioxidants to regulators in cellular signalling and gene expression review: When is an antioxidant not an antioxidant? A review of novel actions and reactions of vitamin C. Free Radical Research. 2005;39:671–686.
Sharma P. Diversity, indigenous uses, threat categorization and conservation prioritization of medicinal plants: A case study from Himachal Pradesh, India. Journal of Biodiversity & Endangered Species; 02. Epub ahead of print; 2014.
DOI: 10.4172/2332-2543.1000134
Rathee P, Rathee D, Rathee D, et al. In-vitro cytotoxic activity of β-Sitosterol triacontenate isolated from Capparis decidua (Forsk.) Edgew. Asian Pacific Journal of Tropical Medicine. 2012;5:225–230.
Dalvi SN, Funde PE, Pokharkar RD, et al. Effect of concentration of KOH, H2O, temp in in-situ transestrification reaction of Sesbania sesban, capparis deciduas seed. Renewable Energy and Power Quality. 2009;1:28–31.
Dar AI, Masar G, Jadhaw V, et al. Isolation and structural elucidation of the novel flavone glycoside from Feronia limonia L. Journal of Pharmacy Research. 2013;7: 697–704.
Zhai X, Zhang C, Zhao G, et al. Antioxidant capacities of the selenium nanoparticles stabilized by chitosan. Journal of Nanobiotechnology; 15. Epub ahead of print; 2017.
DOI: 10.1186/s12951-016-0243-4
Subramaniam N, Muthukrishnan A. Oral mucositis and microbial colonization in oral cancer patients undergoing radiotherapy and chemotherapy: A prospective analysis in a tertiary care dental hospital. J Investig Clin Dent. 2019;10:e12454.
Vadivel JK, Govindarajan M, Somasundaram E, et al. Mast cell expression in oral lichen planus: A systematic review. J Investig Clin Dent. 2019;10:e12457.
Patil SR, Maragathavalli G, Ramesh DNSV, et al. Assessment of maximum bite force in oral submucous fibrosis patients: A preliminary study. Pesqui Bras Odontopediatria Clin Integr. 2020;20:482.
Patil SR, Maragathavalli G, Araki K, et al. Three-rooted mandibular first molars in a Saudi Arabian population: A CBCT study. Pesqui Bras Odontopediatria Clin Integr. 2018;18:e4133.
Patil SR, Yadav N, Al-Zoubi IA, et al. Comparative study of the efficacy of newer antioxitands lycopene and oxitard in the treatment of oral submucous fibrosis. Pesqui Bras Odontopediatria Clin Integr. 2018;18:1–7.
Dave PH, Preetha. Pathogenesis and novel drug for treatment of asthma-a review. Research Journal of Pharmacy and Technology. 2016;9:1519.
Fathima F, Preetha P. Evaluation of thyroid function test in obese patients. Asian Journal of Pharmaceutical and Clinical Research. 2016;9:353.
Preetha S, Packyanathan J. Comparison of the effect of Yoga, Zumba and Aerobics in controlling blood pressure in the Indian population. Journal of Family Medicine and Primary Care. 2020;9:547.
Shruthi M, Preetha S. Effect of simple tongue exercises in habitual snorers. Research Journal of Pharmacy and Technology. 2018;11:3614.
Mollania N, Tayebee R, Narenji-Sani F. An environmentally benign method for the biosynthesis of stable selenium nanoparticles. Research on Chemical Intermediates. 2016;42:4253–4271.
Tayebee R, Pejhan A, Ramshini H, et al. Equisetum arvense as an abundant source of silica nanoparticles. SiO2 /H3 PW12 O40 nanohybrid material as an efficient and environmental benign catalyst in the synthesis of 2-amino-4H-chromenes under solvent-free conditions. Applied Organometallic Chemistry. 2018;32:e3924.
Xu BJ, Chang SKC. A comparative study on phenolic profiles and antioxidant activities of legumes as affected by extraction solvents. Journal of Food Science. 2007; 72:S159–S166.
Gunti L, Dass RS, Kalagatur NK. Phytofabrication of selenium nanoparticles from Emblica officinalis fruit extract and exploring its biopotential applications: Antioxidant, antimicrobial and bio-compatibility. Frontiers in Microbiology; 10. Epub ahead of print; 2019.
DOI: 10.3389/fmicb.2019.00931.
Zia-Ul-Haq M, Ćavar S, Qayum M, et al. Compositional studies: Antioxidant and antidiabetic activities of Capparis decidua (Forsk.) edgew. International Journal of Molecular Sciences. 2011;12:8846–8861.
Boroumand S, Safari M, Shaabani E, et al. Selenium nanoparticles: Synthesis, characterization and study of their cytotoxicity, antioxidant and antibacterial activity. Materials Research Express. 2019;6:0850d8.
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