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A rapid and feasible method of HPTLC is standardized for quantification of anethole in essential oil’s extract and from herbal formulations of fennel seed. The developed densitometric HPTLC method was performed to estimate the existence of anethole in the essential oil, extract and herbal formulations of fennel with the optimized concentration of hexane: Ethyl acetate (8:2%, v/v, mobile phase) on glass coated silica gel 60 F254 plates (20 × 10 cm) scanned with the absorbance of λ260 nm under densitometric condition. The Linearity of regressions revealed a satisfactory relationship between peak area and concentration of anethole in between the range of 100-600 ng/spot. This reliable method was validated as per the ICH guidelines to fulfill the necessary parameters such as accuracy and robustness. The amount of anethole in essential oil (0.098 ± 0.002%), extract (0.101 ± 0.004%) and three herbal formulations A (0.024 ± 0.004%), C (0.019 ± 0.002%) while anethole is not detected in B formulations from fennel seed was completely estimated by the developed method. The standardized methods and its validation gave new insights of HPTLC based detection and quantification of anethole in other aromatic plants as well as in other pharmacological formulations.
Jamwal NS, Kumar S, Rana AC, Phytochemical and pharmacological review on Foeniculum vulgare. 2013;4: 327–341.
Shahat AA, Ibrahim AY, Hendawy SF, Omer EA, Hammouda FM, Abdel-Rahman FH, Saleh MA. Chemical composition, antimicrobial and antioxidant activities of essential oils from organically cultivated fennel cultivars. Molecules. 2011;16(2): 1366-77.
Anand P, Kunnumakara A, Sundaram C, Harikumar K, Tharakan S, Lai O, Sung B, Aggarwal B. Cancer is a preventable disease that requires major lifestyle changes. Pharmaceut. Res. 2008;25: 2097-2116.
Sajomsang W, Gonil P, Tantayanon S. Antibacterial activity of quaternary ammonium chitosan containing mono or disaccharide moieties: Preparation and characterization. Int. J. Biol. Macro-molecules. 2009;44:419-427.
Alam P, Abdel-Kader MS, Alqarni MH, Zaatout HH, Ahamad SR, Shakeel F. Chemical composition of fennel seed extract and determination of fenchone in commercial formulations by GC-MS method. J Food Sci Technol. 2019;56(5): 2395-2403.
Hammouda FM, Saleh MA, Abdel-Azim NS, Shams KA, Ismail SI, Shahat AA, Saleh IA. Evaluation of the essential oil of Foeniculum vulgare Mill (fennel) fruits extracted by three different extraction methods by GC/MS. Afr J Tradit Complement Altern Med. 2014;11(2):277-9.
Renjie L, Zhenhong L, Shidi S. GC-MS analysis of fennel essential oil and its effect on microbiology growth in rats’ intestine. Afr. J. Microbiol. Res. 2010;4: 1319-1323.
Nassar MI, Aboutabl EA, Makled YA, ElKhrisy EA, Osman AF. Secondary metabolites and pharmacology of Foeniculum vulgare Mill. Subsp. Piperitum. Revista Latinoamericana de Química. 2010;38(2):103–112.
Tao Y, He F, Jin K, Wang J, Wang Y, Zhou J, Sun, J, Fang Q. Facile conversion of plant oil (anethole) to a high-performance material. Polym. Chem. 2017;8:2010–2015.
Marinov V, Valcheva-Kuzmanova S. Review on the pharmacological activities of anethole. Scripta scientifica pharmaceutica. 2015;2(2):14-19.
Egyptian Pharmacopoeia (Eng. Text) Cairo University. Cairo, 3rd Edn. 1984;429: 472-733.
International Conference on Harmoni-sation (ICH) of Technical Requirements for registration of pharmaceuticals for human use: Harmonised triplicate guideline on validation of analytical procedures: Methodology, recommended for adoption at Step 4 of the ICH Process on November 1996 by the ICH Steering Committee, IFPMA, Switzerland; 1996.
Bilia AR, Fumarola M, Gallori S, Mazzi G, Vincieri FF. Identification by HPLC-DAD and HPLC-MS analyses and quantification of constituents of fennel teas and decoctions. J Agric Food Chem. 2000;48:4734-8.
Diaz-Maroto MS, Perez-Coello S, Esteban J, Sanz J. Comparison of the volatile composition of wild fennel samples (Foeniculum vulgare Mill.) from Central Spain. J. Agric. Food Chem. 2006;54: 6814-6818.