Anti-proliferative Properties of New Variety Organic Rice MRQ74 Extracts against Colon Cancer Cells: In-vitro Study

Main Article Content

Mohd Adzim Khalili Rohin
Norhayati Abd Hadi
Norhaslinda Ridzwan

Abstract

Aims: To examine the anti-proliferative properties of different extracts of new variety an organic rice MRQ74 towards colon cancer cells: in-vitro study.

Study Design: Experimental study.

Place and Duration of Study: Central Laboratory, Tissue Culture Laboratory, University of Sultan Zainal Abidin, Terengganu from November 2019 until February 2020.

Methodology: The organic rice MRQ74 extracts had been led to tetrazolium salt reduction (MTT) assay and an inhibition concentration of 50 (IC50) value for their cytotoxic potential against colon cancer cells. Meanwhile, cells morphology observation and fluorescence double staining of treatment cells were determined using a light inverted microscope and acridine orange/propidium iodide staining.

Results: Results showed that 50% aqueous ethanol of rice grains gave the lowest IC50 values towards HCT-116 and CT-26 cell lines, while an aqueous solution of rice grains gave the lowest IC50 values towards HT-29 cells (p<0.05). Thoroughly, the treated colon cancer cells had shown morphological alterations after treated with different solvent extracts of an organic rice MRQ74.

Conclusion: The outcomes had observed preliminary results on cancer study for better health by inspiring the consumption of an organic rice MRQ74 and future product developments.

Keywords:
Organic rice MRQ74, colon cancer, cytotoxic, MTT assay, AO/PI staining.

Article Details

How to Cite
Rohin, M. A. K., Hadi, N. A., & Ridzwan, N. (2020). Anti-proliferative Properties of New Variety Organic Rice MRQ74 Extracts against Colon Cancer Cells: In-vitro Study. Journal of Pharmaceutical Research International, 32(12), 55-67. https://doi.org/10.9734/jpri/2020/v32i1230560
Section
Original Research Article

References

Syahrin S, Mohd Rashid R, Abu Kasim A, Tapsir S, Ahmad Shokri O. Persepsi pengguna dan kesanggupan membeli beras Maswangi (MRQ 74). Economics and Technology Management Review. 2008;3:47-56.

Abubakar B, Yakasai HM, Zawawi N, et al. Compositional analyses of white, brown and germinated forms of popular Malaysian rice to offer insight into the growing diet-related diseases. J Food Drug Anal. 2018;26(2):706-15.

Mohd Yusof BN, Abd Talib R, Karim NA. Glycaemic index of eight types of commercial rice in Malaysia. Malays J Nutr. 2005;11(2):151-63.

Abu Bakar NK, Hashim S. Pengaruh jarak tanaman dan umur semaian ke atas prestasi padi wangi tempatan. Jurnal Teknologi. 2014;70:1-6.

MARDI. Pengeluaran padi wangi dalam kawasan luar jelapang padi. 2011-2020.

Available:https://blogmardi.wordpress.com/2011/10/20/pengeluaran-padi-wangi-dalam-kawasan-luar-jelapang-padi/. Malay

Huang MT, Smart RC, Wong CQ, Conney AH. Inhibitory effect of curcumin, chlorogenic acid, caffeic acid and ferulic acid on tumor promotion in mouse skin by 12-O-tetradecanoylphorbol-13-acetate. Cancer Res. 1988;48:5941–6.

Verghese M, Rao DR, Chawan CB, Walker LT, Shackelford L. Anticarcinogenic effect of phytic acid (IP6): Apoptosis as a possible mechanism of action. LWT - Food Sci Technol. 2006;39:1093–8.

Janicke B, Onning G, Oredsson SM. Differential effects of ferulic acid and p-coumaric acid on S phase distribution and length of S phase in the human colonic cell line Caco-2. J Agric Food Chem. 2005;53:6658–65.

Jackson CL, Dreaden TM, Theobald LK, Tran NM, Beal TL, Eid M, Gao MY, Shirley RB, Stoffel MT, Kumar MV, Mohnen D. Pectin induces apoptosis in human prostate cancer cells: Correlation of apoptotic function with pectin structure. Glycobiology. 2007;17:805–19.

Ryan EP. Bioactive food components and health properties of rice bran. J Am Vet Med Assoc. 2011;238:593–600.

Angela JH, Cadie AO, Ajay K, Erica CB, Komal R, Rajesh A, Elizabeth PR. Chemopreventive properties of dietary rice bran; current status and future prospects. Adv Nutr. 2012;3:643-53.

Katyama M, Yoshimi N, Yamada Y, Sakata K, Kuno T, Yoshida K, Qiao Z, Vihn PQ, Iwasaki T, Kobayashi H, et al. Preventive effect of fermented brown rice and rice bran against colon carcinogenesis in male F344 rats. Oncol Rep. 2002;9:817–22.

Phutthaphadoong S, Yamada Y, Hirata A, Tomita H, Hara A, Limtrakul P, Iwasaki T, Kobayashi H, Mori H. Chemopreventive effect of fermented brown rice and rice bran (FBRA) on the inflammation-related colorectal carcinogenesis in ApcMin/+ mice. Oncol Rep. 2010;23:53–9.

Sun W, Xu W, Liu H, Liu J, Wang Q, Zhou J, Dong F, Chen B. Gamma- Tocotrienol induces mitochondria-mediated apoptosis in human gastric adenocarcinoma SGC-7901 cells. J Nutr Biochem. 2009;20:276–84.

Ferlay J, Soerjomataram I, Dikshit R, et al. Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer. 2015;136(5):59-386.

Torre LA, Bray F, Siegel RL, et al. Global cancer statistics. CA-Cancer J Clin. 2015;65(2):87-108.

Melini V, Acquistucci R. Health-promoting compounds in pigmented Thai and wild rice. Foods. 2017;6(1):9.

Mohd Adzim Khalili R, Norhaslinda R, Mimie NJ, Norhayati AH, Syed Ahmad Tajudin TJ, Ahmad Zubaidi AL. Cytotoxicity study and morphological changes of different extraction for Bismillah leaf (Vernonia amygdalina) in human glioblastoma multiforme cell line (U-87). Biomed Res. 2017;28(2):1-7.

Zhang ZS, Li D, Wang LJ, et al. Optimization of ethanol–water extraction of lignans from flaxseed. Sep Purif Technol. 2007;57(1):17–24.

Khalili RMA, Noratiqah JM, Norhaslinda R, et al. Cytotoxicity effect and morphological study of different Duku (Lansium domesticum Corr.) extract toward human colorectal adenocarcinoma cells line (HT-29). Pharmacog J. 2017;9(6):757-61.

Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods. 1983;65(1-2):55–63.

Rohin MAK, Jumli MN, Ridzwan N, Baig AA, Latif AZA, Hadi NA. Effects of Gynura procumbens extracts on anti- proliferative activity and its associated morphological changes of human glioblastoma multiforme cell line (U-87). Pharmacog J. 2018;10(3): 492-6.

Merlin NJ, Parthasarathy V, Santhoshkumar TR. Induction of apoptosis in human breast cancer cell line MCF-7 by phytochemicals from Gmelina asiatica. Afr J Biotechnol. 2010;9(28):4451-6.

Hajiaghaalipour F, Faraj FL, Bagheri E, et al. Synthesis and characterization of a new benzoindole derivative with apoptotic activity against colon cancer cells. Curr Pharm Des. 2017;23(41):6358-65.

Bhebhe M, Fuller TN, Chipurura B, Muchuweti M. Effect of solvent type on total phenolic content and free radical scavenging activity of black tea and herbal infusions. Food Anal Methods. 2015;4(9): 1060-7.

Ye F, Liang Q, Li H, Zhao G. Solvent effects on phenolic content, composition, and antioxidant activity of extracts from florets of sunflower (Helianthus annuus L.). Ind Crops Prod. 2015;76:574–81.

Do QD, Angkawijaya AE, Tran-Nguyen PL, et al. Effect of extraction solvent on the total phenolic content, total flavonoid content, and antioxidant activity of Limnophila aromatica. J Food Drug Anal. 2014;22:296-302.

Dhanani T, Shah S, Gajbhiye NA, Kumar S. Effect of extraction methods on yield, phytochemical constituents and antioxidant activity of Withania somnifera. Arab J Chem. 2017;10(1):S1193-9.

Dailey A, Vuong QV. Effect of extraction solvents on recovery of bioactive compounds and antioxidant properties from macadamia (Macadamia tetraphylla) skin waste. Cogent Food Agric. 2015;1(1): 1-10.

Qu WJ, Pan ZL, Ma HL. Extraction modelling and activities of antioxidants from pomegranate marc. J Food Eng. 2010;99:16–23.

Forster GM, Raina K, Kumar A, et al. Rice varietal differences in bioactive bran components for inhibition of colorectal cancer cell growth. Food Chem. 2013;141(2):1545-52.

Tan BL, Norhaizan ME, Suhania HJ, et al. Antioxidant properties and anti-proliferative effect of brewers’ rice extract (temukut) on selected cancer cell lines. Int Food Res J. 2013;20(5):2117-24.

Tan BL, Norhaizan ME, Yeap SK, Roselina K. Water extract of brewers’ rice induces anti-proliferation of human colorectal cancer (HT-29) cell lines via the induction of apoptosis. Eur Rev Med Pharmacol Sci. 2015;19(6):1022–9.

Kim SJ, Park HR, Park E, Lee SC. Cytotoxic and antitumor activity ofmomilactone B fromrice hulls. J Agric Food Chem. 2007;55(5):1702–6.

Norazalina S, Norhaizan ME, Hairuszah I, Norashareena MS. anticarcinogenic efficacy of phytic acid extracted from rice bran on azoxymethane-induced colon carcinogenesis in rats. Experimental and Toxicol Pathol. 2010;62(3):259–68.

Cai H, Al-Fayez M, Tunstall RG, et al. The rice bran constituent tricin potently inhibits cyclooxygenase enzymes and interferes with intestinal carcinogenesis in