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The present study has been targeted towards; investigation of molecular epidemiology and analysis of antibiotic resistance in different bacterial sp. Total 120 new bacterial isolates has been obtained having majority of bacteria Enterococcus sp. from 4 regional hospitals of 92 patients. The antimicrobial susceptibility test has been performed using 18 different antibiotics and resistant strains have been analyzed. Additionally, the isolated strains were tested for antibiotic resistance and polymerase chain reaction (PCR) has been performed for van A and van B genes. In the series of antimicrobial bacterial species Enterococcus sp. has emerged as one of the potential cause to raise the healthcare problems. This study has significant impact on such kind of molecular epidemiology investigations and may be useful in producing the basic knowledge on the local microorganism to refine and resolve the antimicrobial resistance issues faced by hospitals in the world.
Franz Charles MAP, Michael E. Stiles, Karl Heinz Schleifer, Wilhelm H. Holzapfel. Enterococci in foods—a conundrum for food safety. International Journal of Food Microbiology. 2003;88(2-3):105-122.
Upadhyaya PM, Giridhara KL, Ravi Kumar, Umapathy BL. Review of virulence factors of enterococcus: An emerging nosocomial pathogen. Indian Journal of Medical Microbiology. 2009;27(4):301.
Domig Konrad J, Helmut K. Mayer, Wolfgang Kneifel. Methods used for the isolation, enumeration, characterisation and identification of Enterococcus spp.: 2. Pheno-and genotypic criteria. International Journal of Food Microbiology. 2003; 88(2-3):165-188.
Foka T, Eric F, Ateba CN. Detection of virulence genes in multidrug resistant enterococci isolated from feedlots dairy and beef cattle: Implications for Human Health and Food Safety. Bio Med Research International; 2019.
Haque, Khalid N. Neonatal sepsis in the very low birth weight preterm infants: Part 2: Review of definition, diagnosis and management. J Med Sci. 2010;3(1):11- 27.
Ahmed, Mohamed O, Keith E. Baptiste. Vancomycin-resistant enterococci: A review of antimicrobial resistance mechanisms and perspectives of human and animal health. Microbial Drug Resistance. 2018;24(5):590-606.
Shahraki Shahram, Morteza Rabi Nezhad Mousavi. Determination of virulence factors in clinical multidrug resistance Enterococci isolates at Southeast of Iran. Jundishapur Journal of Microbiology. 2017;10(5).
Esmail, Mona Abdel Monem, Hend M. Abdulghany, Rasha MM Khairy. Prevalence of multidrug-resistant Enterococcus faecalis in hospital-acquired surgical wound infections and bacteremia: concomitant analysis of antimicrobial resistance genes. Infectious Diseases: Research and Treatment. 2019;12: 11786337-19882929.
Arias Cesar A, German A. Contreras, Barbara E. Murray. Management of multidrug-resistant enterococcal infections. Clinical Microbiology and Infection. 2010;16(6):555-562.
Medeiros Aline Weber, Derek Blaese Amorim, Maurício Tavares, Tiane Martin de Moura, Ana Claudia Franco, Pedro Alvesd’ Azevedo, Jeverson Frazzon, Ana Paula Guedes Frazzon. Enterococcus species diversity in fecal samples of wild marine species as determined by real-time PCR. Canadian Journal of Microbiology. 2017;63(2):129-136.
Jones Mark E, Deborah C, Draghi Clyde Thornsberry, James A. Karlowsky, Daniel F. Sahm, Richard P. Wenzel. Emerging resistance among bacterial pathogens in the intensive care unit–a European and North American Surveillance study (2000–2002). Annals of Clinical Microbiology and Antimicrobials. 2004; 3(1):14.
Ferguson Donna, Caterina Signoretto. Environmental persistence and naturalization of fecal indicator organisms. In Microbial Source Tracking: Methods, Applications and Case Studies. Springer, New York, NY. 2011;379-397.
Zaheer Rahat, Shaun R. Cook, Ruth Barbieri, Noriko Goji, Andrew Cameron, Aaron Petkau, Rodrigo Ortega Polo, et al. Surveillance of Enterococcus spp. reveals distinct species and antimicrobial resistance diversity across a one-health continuum. Scientific Reports. 2020;10(1): 1-16.
Zhu Xiaofei, Qianqian Wang, Chengfei Zhang, Gary SP Cheung, Ya Shen. Prevalence, phenotype and genotype of Enterococcus faecalis isolated from saliva and root canals in patients with persistent apical periodontitis. Journal of endodontics. 2010;36(12):1950-1955.
Sassone L, Fidel R, Figueiredo L, Fidel S, Faveri M, Feres M. Evaluation of the microbiota of primary endodontic infections using checkerboard DNA–DNA hybridization. Oral Microbiology and Immunology. 2007;22(6):390-397.
Zoletti Gustavo O, Eliezer M. Pereira, Ricardo P. Schuenck, Lúcia M. Teixeira, José F. Siqueira Jr, Kátia Regina N. dos Santos. Characterization of virulence factors and clonal diversity of Enterococcus faecalis isolates from treated dental root canals. Research in Microbiology. 2011;162(2):151-158.
Lins, Renata Ximenes, Aurimar de Oliveira Andrade, Raphael Hirata Junior, Melanie J. Wilson, Michael A. O. Lewis, David W. Williams, Rivail Antonio Sergio Fidel. Antimicrobial resistance and virulence traits of Enterococcus faecalis from primary endodontic infections. Journal of Dentistry. 2013;41(9):779-786.
Barbolla RE, Centrón D, Maimone S, Rospide F, Salgueira C, Altclas J, Catalano M. Molecular epidemiology of Acinetobacter baumannii spread in an adult intensive care unit under an endemic setting. American Journal of Infection Control. 2008;36(6):444-452.
Kristich CJ, Rice LB, Arias CA. Enterococcal infection—treatment and antibiotic resistance; 2014.
Farman Muhammad, Muhammad Yasir, Rashad R. Al-Hindi, Suha A. Farraj, Asif A. Jiman-Fatani, Maha Alawi, Esam I. Azhar. Genomic analysis of multidrug-resistant clinical Enterococcus faecalis isolates for antimicrobial resistance genes and virulence factors from the Western Region of Saudi Arabia. Antimicrobial Resistance & Infection Control. 2019;8(1):55.
El-Kersh TA, Marie MA, Al-Sheikh YA, Al-Agamy MH, Al-Bloushy AA. Prevalence and risk factors of early fecal carriage of Enterococcus faecalis and Staphylococcus spp and their antimicrobial resistant patterns among healthy neonates born in a hospital setting in Central Saudi Arabia. Saudi Med J. 2016;37(3):280.
Salem-Bekhit MM, Moussa IMI, Elsherbini MMAM, AlRejaie S. Increasing prevalence of high-level gentamicin resistant enterococci: An emerging clinical problem. Afr J Microbiol Res. 2011;5(31):5713– 20.
Fernández-Hidalgo N, Almirante B, Gavaldà J, Gurgui M, Peña C, de Alarcón A, Ruiz J, Vilacosta I, Montejo M, Vallejo N. Ampicillin plus ceftriaxone is as effective as ampicillin plus gentamicin for treating Enterococcus faecalis infective endocarditis. Clin Infect Dis. 2013;56(9): 1261–8.
Richey E, Waters P, Jovic M, Rakhman C. Treatment of ampicillin-resistant enterococcus faecium urinary tract infections. Fed Pract. 2015;32:20–3.
Salem-Bekhit MM, Moussa IM, Muharram MM, Alanazy FK, Hefni HM. Prevalence and antimicrobial resistance pattern of multidrug-resistant enterococci isolated from clinical specimens. Indian J Med Microbiol. 2012;30(1):44–51.
Sievert DM, Ricks P, Edwards JR, Schneider A, Patel J, Srinivasan A, Kallen A, Limbago B, Fridkin S. Antimicrobial-resistant pathogens associated with healthcare-associated infections summary of data reported to the National Healthcare Safety Network at the Centers for Disease Control and Prevention, 2009–2010. Infect Control HospEpidemiol. 2013;34(1): 1–14.
Hollenbeck BL, Rice LB. Intrinsic and acquired resistance mechanisms in enterococcus. Virulence. 2012;3(5):421–433.
Shankar N, Lockatell CV, Baghdayan AS, Drachenberg C, Gilmore MS, Johnson DE. Role of Enterococcus faecalis surface protein Esp in the pathogenesis of ascending urinary tract infection. Infection and Immunity. 2001;69(7):4366–4372.
Padmasini E, Padmaraj R, Ramesh SS. High level aminoglycoside resistance and distribution of aminoglycoside resistant genes among clinical isolates of Enterococcus species in Chennai, India. Sci World J.; 2014.
Cho S, Hiott LM, McDonald JM, Barrett JB, McMillan EA, House SL, Adams ES, Frye JG, Jackson CR. Diversity and antimicrobial resistance of Enterococcus from the Upper Oconee Watershed, Georgia. Journal of Applied Microbiology. 2020;128(4):1221-1233.
Quale J, Landman D, Saurina G, et al. Manipulation of a hospital antimicrobial formulary to control an outbreak of vancomycin-resistant enterococci. Clinical Infectious Diseases. 1996;23:1020–1025.
Samra Siddiqui MS, Faisal SM, Akhter A, Sarim KM, Khan M, Ahmad I, Ahmad I. Molecular and phenotypic characterization of the vancomycin-resistant gene in bacterial isolates acquired from catheter tips. Cell Mol Biol (Noisy le Grand). 2017;63(12).
Boyle JF, Soumakis SA, Rendo A, et al. Epidemiologic analysis and genotypic characterization of a nosocomial outbreak of vancomycin-resistant entero; 1993.