Effect of Methanolic Extract of Securigera securidaca as Antioxidant and Antibacterial Activities

Main Article Content

Ghassab M. Al- Mazaideh
Saleh A. Al- Quran

Abstract

In the present work, the phytochemical screening, polyphenolic content, antibacterial activity and antioxidant activity of Securigera securidaca seeds in methanol were carried out. Phytochemical analysis of seeds showed the presence of alkaloids, flavonoids, saponins, terpenoids, steroids and glycosides. Total phenolic content was estimated by Folin Ciocalteau method and the result showed the highest phenolic content of 62.28 mg/g. Methanolic extract was screened for antibacterial activity by disc diffusion method and it found to be potent. The MIC of methanol extract identified by broth dilution method showed a MIC value of 0.25 mg/ml for both E. coli and Kl. Oxytoca, and also 0.5 mg/ml for both S. aureus and S. epidermis. The antioxidant effect of the seeds was tested by DPPH scavenging activity as in vitro assay. The extract had potent inhibitory activity (IC50) value of 0.057 mg/ml. The finding experimental results showed that methanolic extract of Securigera securidaca is important as a source of antibacterial activity and polyphenolic antioxidants.

Keywords:
Securigera securidaca, antioxidant, anticorrosion, polyphenolic content, MIC

Article Details

How to Cite
Mazaideh, G. M. A.-, & Quran, S. A. A.-. (2020). Effect of Methanolic Extract of Securigera securidaca as Antioxidant and Antibacterial Activities. Journal of Pharmaceutical Research International, 32(11), 10-17. https://doi.org/10.9734/jpri/2020/v32i1130498
Section
Original Research Article

References

Al-Quran SA, Al-Mazaideh GM. Inhibitive action of chamomile extract on the corrosion of Iron: Density Functional Theory, Mor. J. Chem. 2018;6(1):195-202.

Wedian F, Al-Qudah MA, Al-Mazaideh GM. Corrosion inhibition of copper by Capparis spinosa L. extract in strong acidic medium: Experimental and Density Functional Theory. Int. J. Electrochem. Sci. 2017;12: 4664-4676.

Al-Mazaideh GM, Carbohydrates as green corrosion inhibitors of cooper: Ab initio Study, Jord. J. Chem. 2017;12(4):189-200.

Al-Mazaideh GM, Ababneh TS, Abu-Shandi KH, Jamhour RMAQ, Ayaal Salman HJ, Al-Msiedeen AM, Khalil SM. DFT calculations of mesembryanthemum nodiflorum compounds as corrosion inhibitors of Aluminium. Phy. Sci. Inter. J. 2016;12(1):1-7.

Al-Mazaideh GM, Al-Zereini WA, Al-Mustafa AH, Khalil SM. The effect of nitro maleimides from a marine vibrio species compounds as a source of environment-tally friendly corrosion inhibitors for metals: A computational Study. Adv. Envi. Biol. 2016;10(8):159-168.

Abu-Shandi K, Al-Rawashdeh B, Al-Mazaideh GM, Abu-Nameh E, Al-Amro A, Al-Soufi H, Al-Ma'abreh A, Al-Dawdeyah A, A novel strategy for the identification of the medicinal natural products in rubus fruticosus plant by Using GC/MS technique: A study on leaves, stems and roots of the plant. Adv. Anal. Chem. 2015;5(2):31-41.

Anesini C, Perez C, Screening of plants used in argentine folkmedicine for antimicrobial activity. J. Ethnopharmacol. 1993;39(2):119-128.

Gharaman A, Flore de iranen couleurs naturelle, Tehran university press, Tehran. 1993;Vol12:1478.

Al-Hachim GM, Maki B. Effect of Securigera securidaca on electroshock seizure threshold in mice. Psychological Reports. 1969;24:551–553.

Hosseinzadeh H, Ramezani M, Danaei AR. Antihyperglycaemic effect and acute toxicity of Securigera securidaca L. seed extracts in mice. Phytotherapy Research. 2002;16:745–747.

Behbahani M, Sayedipour S, Pourazar A, Shanehsazzadeh M. In vitro anti- HIV-1 activities of kaempferol and kaempferol-7-Oglucoside isolated from Securigera securidaca. Res. Pharm. Sci., Decenber. 2014;9(6):463-469.

Garjani A, Fathiazad F, Zakheri A, et al. The effect of total extract of Securigera securidaca L. seeds on serum lipid profiles, antioxidant status, and vascular function in hypercholesterolemic rats, J. Ethnopharmacology. 2009;126(3): 525–532.

Garjani A, Fathiazad F, Zakheri A, Allaf Akbari N, Azarmie Y, Fakhrjoo A et al. The effect of total extract of Securigera securidaca L. seeds on serum lipid profiles, antioxidant status, and vascular function in hypercholesterolemic rats. J.Ethnopharmacol. 2009;126:525–32.

Zatula VV, Kovalev IP, Kolesnikov DG. Configuration of securigenin and securigenol. Chem. Nat Compd. 19695:111-112.

Komissarenko AN, Kovalev VN, Hydroxycoumarins and flavones of Securigera securidaca, Chem. Nat. Comp. 1987;23(2):252-252.

Choi CW, Kim SC, Hwang SS, Choi BK, Ahn HJ, Lee MY, Park SH, Kim SK. Antioxidant activity and free radical scavenging capacity between Korean medicinal plants and flavonoids by assay-guided comparison. Plant Science. 2002;163:1161–1168.

Sadat-Ebrahimi S, Mir MH, Amin G, Hajimehdipoor H. Identification of amino acids in Securigera securidaca, a popular medicinal herb in Iranian folk medicine. Res. J. Pharmacogn. 2014;1(1):23-26.

Tofighi Z, Molazem M, Doostdar B, Taban P, Shahverdi A, Samadi N, Yassaa N. Antimicrobial activities of three medicinal plants and investigation of flavonoids of Tripleurospermum disciforme, Iranian J. Pharm. Res. 2015; 14(1):225-231.

Jaradat N, Abualhasan M, Ali I. Comparison of anti-oxidant activities and exhaustive extraction yields between wild and cultivated Cyclamen persicum, Malva sylvestris and Urtica pilulifera leaves. J. Appl. Pharm Sci. 2015;5(4):101-107.

Jaradat N, Hussen F, Al Ali A. Preliminary phytochemical screening, quantitative estimation of total flavonoids, total phenols and antioxidant activity of Ephedra alata decne. J. Mater Environ. Sci. 2015;6(6): 1771-1779.

Evans WC. Trease and Evans' pharmacognosy. United States: Elsevier Health Sciences; 2009.

Kumar S, Rajesekaran T, Shanmugam P, Kumar VM. Phytochemical constituents and antibacterial activivties of Elaeocarpus ganitrus Roxb. and canthium parviflora linn. leaves. Advanced Biotech. 2007;6: 23-25.

Malik CP, Singh MB. Plant enzymology and histo-enzymology: A text Manual. Kalyani Publishers, New Delhi. 1980;286.

Sakanaka S, Tachibana Y, Okada Y. Preparation and antioxidant properties of extracts of Japanese persimmon leaf tea (kakinoha-cha). Food Chemistry. 2005; 89(4):569–575.

Bauer AW, Krirby WM, Sherris JC, Turck M. Antibiotic susceptility testing by a standardized single disc method. Amer. J. Clin. Pathol. 1966;45(4):493-496.

Oreky NS, Nakahara K, Antibacterial activity of extracts from some edible plants commonly consumed. Asia International J. Food Microbiology. 2003;80:223-230.

Dharajiya D, Patel P, Patel M, Moitra N. In vitro antimicrobial activity and qualitative phytochemical analysis of With Ania somnifera (L.) dunal extracts. Int. J. Pharm Sci. Rev. Res. 2014;27:349-54.

Kaviarasan S, Naik GH, Gangabhagirathi R, Anuradha CV, Priyadarsini KI. In vitro studies on antiradical and antioxidant activities of fenugreek (Trigonella foenum-graecum) seeds. Food Chemistry. 2007; 103(1):31–37.

Chung YC, Chang C, Chao WW, Lin CF, Chou ST. Antioxidative activity and safety of the 50 ethanolic extracts from red beam fermentd by Bacillus subtilis MR-K1. J. Agricultural and Food Chemistry. 2002; 50(8):2454-2458.

Ganguly R, Mishra P, Sharma A. Microbes and infection. Indian, J. Microbiol. 2001;41:211-213.

Igbinosa OO, Igbinosa EO, Aiyegoro OA. Antimicrobial activity and phytochemical screening of stem bark extracts from Jairopha curcas (Linn), Afr. J. Pharmacol. 2009;3:58-62.

Kumar GS, Jayaveera KN, Kumar ACK, Sanjay UP, Swamy BMV and Kumar DVK. Antimicrobial effects of acne-inducing bacteria. Tropical, J. Pharma. Res. 2007;6: 717-723.

Yadav R, Tiwari R, Chowdhary P, Pradhan C. A pharmacognostical monograph of Trigonella foenum-graecum seeds. Int. J. Pharm. Pharm. Sci. 2011; 3(5):442-445.

Komissarenko AN, Kovalev VN. Hydroxycoumarins and flavones of Securigera securidaca. Chem. Nat. Compd. 1987;23:252.