Chemo-typing of Three Egyptian Rosemary Oil for Their Chemical Composition and Anti-oxidant Activity

Main Article Content

Menna I. Elshorbagy
Marwa Elsbaey
Hany N. Baraka
Mohamed Farid Lahloub


Aims: To study the chemical composition of volatile oil samples from different place in Egypt (Mansoura, Gamsa and Assuit) and study their antioxidant activity determined by DPPH assay.

Study Design: Hydro distillation of volatile oil samples and their GC/MS analysis and determination of their antioxidant activity by DPPH assay.

Place and Duration of Study: Department of Pharmacognosy, Faculty of pharmacy, Mansoura university, Egypt, between June 2015 and November 2017.  

Methodology: The essential oil was isolated by hydro-distillation for 5 h using a Clevenger-type all-glass apparatus according to the standard procedure of the European pharmacopeia and volatile oils analysis was performed by GC and GC-MS. GC analysis was carried out using Focus-DSQ-ӀI GC/MS instrument (Thermo Scientific, MA, USA) equipped with TR-5 fused silica column (30 m × 0.25 mm, film thickness 0.25 µm).

Results: The yield of the essential oils of three rosemary plants growing in Mansoura (RM), Gamsa (RG) and Assiut (RA) were 0.20% v/w, 0.32% v/w and 0.24% v/w, respectively. Their chemical composition was analyzed by GC/MS, RM and RG were found to be α-pinene dominated chemotypes, 32.4% and 29.6%, respectively, meanwhile RA was camphor dominated chemotype (17.2%). Furthermore, their antioxidant activity was determined by DPPH assay. Their IC50 values of essential oils of RM, RG and RA were 8.66 ± 0.7, 8.18 ± 0.5 and 9.74 ± 0.2, respectively.

Conclusion: The GC/MS spectral data revealed the considerable difference between the chemical composition of essential oil constituents of RM, RG and RA which lead to different chemotypes. The present results also demonstrate that REO obtained from different areas in Egypt exhibited free radical scavenging activity determined by DPPH assay due to the synergistic effect between their constituents.

Rosmarinus officinalis L., essential oil, GC/MS, anti-oxidant activity

Article Details

How to Cite
Elshorbagy, M. I., Elsbaey, M., Baraka, H. N., & Lahloub, M. F. (2020). Chemo-typing of Three Egyptian Rosemary Oil for Their Chemical Composition and Anti-oxidant Activity. Journal of Pharmaceutical Research International, 32(3), 9-14.
Original Research Article


Stefanovits-bányai E, Tulok MH, Hegedûs A, Renner C, Varga IS. Antioxidant effect of various rosemary (Rosmarinus officinalis L.) clones. Acta Biologica Szegediensis. 2003;47:111–13.

Rašković AL, Milanović I, Pavlović N, Ćebović T, Vukmirović S, Mikov M. Antioxidant activity of rosemary (Rosmarinus officinalis L.) essential oil and its hepatoprotective potential. BMC Complementary and Alternative Medicine. 2014;(14):225.

Amin T, Naik HR, Hussain SZ. Chemotyping the essential oil in different rosemary (Rosmarinus officinalis L.) plants grown in Kashmir Valley. Biosciences, Biotechnology Research Asia. 2017; 14(3):1025–31

Neito G, Ros G and Castillo J. Biological activities of three essential oils of the Lamiaceae Family. Medicines. 2017;4(4):63.

Ozcan MM, Chalchat JC. Chemical compostion and anti fungal activty of rosemary (Rosmarinus officinalis L.) oil from Turkey. International Jornal of Food Sceience and Nutrition. 2008;691-98.

Hussain AI, Anwar F, Ali S, Chatha S, Jabbar A, Mahboob S, Nigam PS. Rosmarinus officinalis essential oil: Antiproliferative, antioxidant and antibacterial activities. Brazilian Journal of Microbiology. 2014;41(4):1070–78.

Bousbia N, Vian MA, Ferhat MA, Petitcolas E, Meklati BY, Chemat F. Comparison of two isolation methods for essential oil from rosemary leaves: Hydrodistillation and microwave hydrodiffusion and gravity. Food Chemistry. 2009;114(1):355–62.

Chalchat JC, Garry RP, Michet A, Benjilali B, Chabart JL. Essential oils of rosemary (Rosmarinus officinalis L.). the chemical composition of oils of various origins (Morocco, Spain, France). Journal of Essential Oil Research. 1993;(6):613–18.

Strasbourg, 7th ed. European directorate for the quality of medicines & healthcare (EDQM). European Pharmacopoeia; 2013.

Adam RP. 4th ed, Allured Books, IL, USA. Identification of essential oil components by gas chromatography/mass Spectro-metry; 2009.

Ojeda- Sana AM, van Baren CM, Elechosa MA, Juárez MA, Moreno S. New insights into antibacterial and antioxidant activities of rosemary essential oils and their main components. Food Control 31. 2013;(1):189–95.

Tisserand R, Young R. 2nd ed. essential oil safety. A guide for health care professionals; 2014.

Sebranek JG, Sewalt VJH, Robbons KLF, Houser TA. Comparison of natural rosemary extract and BHA/BHT for relative antioxidant effectiveness in pork sausage. Meat Science. 2005;289-296.

Wang W, Li N, Zu Y, Efferth T. Anti-bacterial activity and anticancer activity of Rosmarinus officinalis L. essential oil compared to that of its main components. Molecules. 2012;17:2704-2713.