Antibacterial Effects of Hydro-alcoholic Extract of Pennyroyal, Cinnamon and Rhubarb on Klebsiella pneumoniae and Staphylococcus aureus: An In-vitro Study
Journal of Pharmaceutical Research International,
Background: Medicinal plants have been used for many different purposes in the past. These plants may produce antimicrobial metabolites that affect the natural growth of microorganisms. The aim of this research was to study the antibacterial properties of rhubarb, cinnamon and pennyroyal on Staphylococcus aureus and Klebsiella pneumoniae.
Materials and Methods: In this study, the effect of hydro-alcoholic extract of pennyroyal, cinnamon and rhubarb on Staphylococcus aureus and Klebsiella pneumoniae was investigated after their collection and extraction process. The Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC) of this extract on bacteria were also measured.
Results: The MIC of hydro alcoholic extract of pennyroyal was above 400 mg/ml for both Klebsiella pneumoniae and Staphylococcus aureus. Also the MBC has been reported 400 and 500 mg/dL, respectively. The MIC of cinnamon extract for Klebsiella pneumoniae and Staphylococcus aureus were reported 250 and higher and 500 and higher, respectively. Also, the MBC was reported 500 and 600 mg/ml, respectively. Klebsiella pneumoniae was resistant to rhubarb plant extract, and the MIC and the MBC for Staphylococcus aureus were reported 200 mg/ml and higher and 400 mg/ml and higher, respectively.
Conclusion: The results of this study demonstrated that the hydro alcoholic extract of cinnamon, pennyroyal and rhubarb has anti bacterial effect against Klebsiella pneumoniae and Staphylococcus aureus.
- Klebsiella pneumoni
- Staphylococcus aureus.
How to Cite
Chambers HF. Methicillin resistance in staphylococci: Molecular and biochemical basis and clinical implications. CMR. 1997;10(4):781-91.
Cunha B. Methicillin-resistant Staphylococcus aureus: Clinical manifestations and antimicrobial therapy. Clin Microbiol Infect. 2005;11:33-42.
George D, Bhat SS, Antony B. Comparative evaluation of the antimicrobial efficacy of Aloe vera tooth gel and two popular commercial toothpastes: An in vitro study. Gen Dent. 2009;57(3):238-41.
Ranade AN, Wankhede SS, Ranpise NS, Mundada MS. Development of bilayer floating tablet of amoxicillin and Aloe vera gel powder for treatment of gastric ulcers. AAPS PharmSciTech. 2012;13(4):1518- 23.
Saeedi M, Ebrahimzadeh MA, Morteza-Semnani K, Âkha O, Rabiei K. Evaluation of antibacterial effect of ethanolic extract of Foeniculum vulgare Mill. JMUMS. 2010;20(77):88-91.
Gulluce M, Sahin F, Sokmen M, Ozer H, Daferera D, Sokmen A, et al. Antimicrobial and antioxidant properties of the essential oils and methanol extract from Mentha longifolia L. ssp. longifolia. Food Chem. 2007;103(4):1449-56.
Yano Y, Satomi M, Oikawa H. Antimicrobial effect of spices and herbs on Vibrio parahaemolyticus. Int J Food Microbiol. 2006;111(1):6-11.
Andrews JM. Determination of minimum inhibitory concentrations. J Antimicrob Chemother. 2001;48(Suppl_1):5-16.
Khaledi M, Asadi-Samani M, Mahmoodi-Kouhi A, Gholipour A. Antibacterial effect of the hydroalcoholic extracts of four Iranian medicinal plants on Staphylococcus aureus and Acinetobacter baumanii. Int. J. Pharm. Phytopharm. Res. 2017;7(2):10-4.
Bowersox J. Experimental staph vaccine broadly protective in animal studies. NIH News. 1999;27.
Jalal Pour S, Kasra Kermanshahi R, Nouhi A, Zarkesh Isfahani H. Comparing the frequency of â-lactamase enzyme in isolated nosocomial infectious bacteria. Zahedan. J. Res. Med. Sci. 2010;12(4):3-10.
Groopman J. The new generation of resistant infections is almost impossible to treat. Medical Dispatch, Superbugs; 2008.
Guo L, Guo J, Xu F. Optimized extraction process and identification of antibacterial substances from Rhubarb against aquatic pathogenic Vibrio harveyi. 3 Biotech. 2017;7(6):377.
Nassan M, Mohamed E, Abdelhafez S, Ismail T. Effect of clove and cinnamon extracts on experimental model of acute hematogenous pyelonephritis in albino rats: Immunopathological and antimicrobial study. Int J Immunopathol Pharmacol. 2015;28(1):60-8.
Soltan Dallal M, Yazdi M, Aghaamiri S, Haghighat Khajavi S, Abedi Mohtasab T, Amin Harati F, et al. Antimicrobial effect of Zataria multiflora and Rosemarinus officinalis on antibiotic-resistant Staphylococcus aureus strains isolated from food. J Med Plant Res. 2014;4(52):41-7.
Sharifi A, Naghmachi M, Jahedi S, Khosravani S. A study on antimicrobial effects of Plantago psyllium. Armaghane Danesh Bimonthly Journal. 2011;16(2):191-9.
Lu C, Wang H, Lv W, Ma C, Xu P, Zhu J, et al. Ionic liquid-based ultrasonic/ microwave-assisted extraction combined with UPLC for the determination of anthraquinones in Rhubarb. Chromatographia. 2011;74(1-2):139.
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