Development of High-performance Thin-layer Chromatography (HPTLC) Validated Method for Simultaneous Quantification of Eucalyptol and α-Pinene in Lamiaceae Plants

Main Article Content

Ahmed Ibrahim Foudah
Prawez Alam
Aftab Alam
Mohammed Ayman Salkini
Mohammed Hamed Alqarni
Hasan Soliman Yusufoglu

Abstract

Aim and Objective: Several plants from Lamiaceae family are used in the Saudi Arabia as a condiment and food preparation, and are generally used in the traditional preparation to treat various diseases, including anti-inflammatory, antioxidant, and microbial infections. Some of Lamiaceae species such as Mentha longifolia, Rosemarinus officinalis and Salvia officinalis having pharmacological active compounds such as α-pinene and eucalyptol. The aim of present study was to develop an accurate and precise chromatographic technique for quantification of α-pinene and eucalyptol in the Lamiaceae plants. 

Methods: The high-performance thin layer chromatography (HPTLC) method was developed as per International Conference on Harmonization (ICH) guideline.

Results: Simultaneous determination of α-pinene and eucalyptol was achieved by developing a densitometric analysis of high-performance thin layer chromatography (HPTLC). Silica gel 60 F254 glass-backed plates (E-Merck, Germany, 0.2 mm layers) as stationary phase and mixture n-hexane: ethyl acetate 8 : 2 (%, v/v) as mobile phase were used to produce a sharp, symmetrical and well-resolved peak at an Rf value of 0.19 ± 0.02 and 0.52 ± 0.04 for α-pinene and eucalyptol, respectively. Linearly range for α-pinene was 100–700 ng/spot (r2 = 0.9988), whereas that for eucalyptol was 1000–7000 ng/spot (r2 = 0.9987).

Conclusion: The developed method was found to be a simple, accurate, and precise, and it may be used to simultaneously analyses of many medicinal plants samples containing α-pinene and eucalyptol.

Keywords:
Eucalyptol, α-pinene, Mentha longifolia, Rosemarinus officinalis, Salvia officinalis, HPTLC and ICH.

Article Details

How to Cite
Foudah, A. I., Alam, P., Alam, A., Salkini, M. A., Alqarni, M. H., & Yusufoglu, H. S. (2019). Development of High-performance Thin-layer Chromatography (HPTLC) Validated Method for Simultaneous Quantification of Eucalyptol and α-Pinene in Lamiaceae Plants. Journal of Pharmaceutical Research International, 31(6), 1-11. https://doi.org/10.9734/jpri/2019/v31i630354
Section
Original Research Article

References

Gottardi D, Bukvicki D, Prasad S, Tyagi AK. Beneficial effects of spices in food preservation and safety. Front Microbiol. 2016;7:1394.

Mamadalieva NZ, Akramov DK, Ovidi E, Tiezzi A, Nahar L, Azimova SS, Sarker SD. Aromatic medicinal plants of the lamiaceae family from Uzbekistan: ethnopharmacology, essential oils composition, and biological activities. Medicines (Basel). 2017;4:8.

Anwar A, Alkharfy KM, Najeeb-ur-Rehman, Adam EHK, Gilani A. Chemo-geographical variations in the composition of volatiles and the biological attributes of Mentha longifolia (L.) Essential oils from Saudi Arabia. Int J Pharmacol. 2017; 13:408-424.

Arraiza MP, Arrabal C, López JV. Seasonal variation of essential oil yield and composition of sage (Salvia officinalis L.) grown in Castilla - La Mancha (Central Spain). Not Bot Horti Agrobot ClujNapoca. 2012;40:106-108.

Satyal P, Jones TH, Lopez EM, McFeeters RL, Ali NA, Mansi I, Al-Kaf AG, Setzer WN. Chemotypic characterization and biological activity of Rosmarinus officinalis. Foods. 2017;6:20.

Hussain AI, Anwar F, Chatha SA, Jabbar A, Mahboob S, Nigam PS. Rosmarinus officinalis essential oil: Antiproliferative, antioxidant and antibacterial activities. Braz. J. Microbiol. 2010;4:1070-1078.

Damyanova S. Mollova S, Stoyanova A, Gubenia O. Chemical composition of Salvia officinalis L. essential oil from Bulgaria. Ukr. Food J. 2016;5:695-700.

Berthold-Pluta A, Stasiak-Różańska L, Pluta A, Garbowska M. Antibacterial activities of plant-derived compounds and essential oils against Cronobacter strains. Eur Food Res Technol. 2019;245:1137-1147.

Kaushik D, Kaushik P, Kumar A, Rana AC, Sharma C, Aneja KR. GC-MS analysis and antimicrobial activity of essential oil of Pinus roxburghii Sarg. from Northern India. J Essent Oil Bear Pl. 2013;16:563-567.

Wajs-Bonikowska A, Szoka Ł, Karna E, Wiktorowska-Owczarek A, Sienkiewicz M. Abies concolor seeds and cones as new source of essential oils-composition and biological activity. Molecules. 2017;22: 1880.

Pinheiro Mde A, Magalhães RM, Torres DM, Cavalcante RC, Mota FS, Oliveira Coelho EM, Moreira HP, Lima GC, Araújo PC, Cardoso JH, de Souza AN, Diniz LR. Gastroprotective effect of alpha-pinene and its correlation with antiulcerogenic activity of essential oils obtained from Hyptis species. Pharmacogn Mag. 2015; 11(41):123–130.

Ueno H, Shimada A, Suemitsu S, Murakami S, Kitamura N, Wani K, Matsumoto Y, Okamoto M, Ishihara T. Attenuation effects of alpha-pinene inhalation on mice with dizocilpine-induced psychiatric-like behaviour. Evid Based Complement Alternat Med. 2019;2019: 2745453.

De Vincenzi M, Silano M, De Vincenzi A, Maialetti F, Scazzocchio B. Constituents of aromatic plants: Eucalyptol. Fitoterapia. 2002;73:269-75.

Swamy MK, Akhtar MS, Sinniah UR. Antimicrobial properties of plant essential oils against human pathogens and their mode of action: An updated review. Evid Based Complement Alternat Med. 2016; 3012462.

Cha JD, Kim YH, Kim JY. Essential oil and 1,8-cineole from Artemisia lavandulaefolia Induces apoptosis in KB cells via mitochondrial stress and caspase activation. Food Sci. Biotechnol. 2010;19: 185-191.

Juergens UR. Anti-inflammatory properties of the monoterpene 1.8-cineole: Current evidence for co-medication in inflammatory airway diseases. Drug Res (Stuttg). 2014;64:638-46.

Kim KY, Seo HJ, Min SS, Park M, Seol GH. The effect of 1,8-Cineole inhalation on preoperative anxiety: A randomized clinical trial. Evid Based Complement Alternat Med. 2014;2014:820126.

Xu J, Hu ZQ, Wang C, Yin ZQ, Wei Q, Zhou LJ, Li L, Du YH, Jia RY, Li M, Fan QJ, Liang XX, He CL, Yin LZ. Acute and subacute toxicity study of 1,8-cineole in mice. Int J Clin Exp Pathol. 2014;7:1495-501.

Shaha A, Salunkhe VR. Development and validation of a high performance thin layer chromatographic method for determination of 1, 8-Cineole in Callistemon Citrinus. Pharmacognosy Res. 2014;6: 143–147.

Alam A, Majumdar RS. Antioxidant activity of essential oil of three cultivars of Amomum subulatum and standardization of high performance thin layer chromatography (HPTLC) method for the estimation of 1,8-cineole. Afr. J. Biotechnol. 2018;17:1129-1137.

Patra KC, Pareta SK, Harwansh RK, Kumar KJ. A validated HPTLC method for estimation of Eucalyptol in polyherbal formulations. Pharm Lett. 2010;2(6):316-320.

Alam P, Alqasoumi SI, Abdel-Kader MS. Simultaneous determination of menthol and eucalyptol by the densitometric HPTLC method in some external analgesic Formulations. J Chromatogr Sci. 2016;54:58-63.

Porel A, Sanyal Y, Kundu A. Simultaneous HPLC determination of 22 components of essential oils; Method robustness with experimental design. Indian J Pharm Sci. 2014;76:19-30.

Esfahanizadeh M, Ayatollahi SA, Goodarzi A, Bayat M, Ata A, Kobarfard F. Development and validation of a GC/MS method for simultaneous determination of 7 monoterpens in two commercial pharmaceutical dosage forms. Iran J Pharm Res. 2018;17:24–32.

Yusufoglu HS, Foudah AI, Alam P, Alam A, Salkini MA, Alqarni MH. Development a validated HPTLC method for quantification of linalool in the oils and extracts of Saudi Arabian Ocimum basilicum and Lavandula angustifolia. Indo Am. j. Pharm. 2019;06: 14408-14417.

International Conference on Harmonisation (ICH) ICH harmonised triplicate guideline on validation of analytical procedures. In: International conference on harmonisation of technical requirements for registration of pharmaceuticals for human use; Switzerland: ICH; 2010.

Guetat A, Al-Ghamdi FA, Osman AK. 1, 8-Cineole, α-Pinene and verbenone chemotype of essential oil of species Rosmarinus officinalis L. from Saudi Arabia. Int. J. Herb. Med. 2014;2:137-141.

Takayama C, De-Faria FM, Almeida ACA, Dunder RJ, Manzo LP, Socca EAR, Batista LM, Salvador MJ, Souza-Brito ARM, Luiz-Ferreira A. Chemical composition of Rosmarinus officinalis essential oil and antioxidant action against gastric damage induced by absolute ethanol in the rat. Asian. Pac. J. Trop. Biomed. 2016;6:677-681.

Burham BO, Osman OA, Nour AAM. Chemical composition and antibacterial activity of essential oil of Mentha longifolia Leaf from Albaha Area Southern Saudi Arabia. Asian J Biol Life Sci. 2019;8:48- 52.

Miguel G, Cruz C, Faleiro ML, Simões MT, Figueiredo AC, Barroso JG, Pedro LG. Salvia officinalis L. essential oils: effect of hydrodistillation time on the chemical composition, antioxidant and antimicrobial activities. Nat Prod Res. 2011;25:526-41.

Jakovljević M, Jokić S, Molnar M, Jašić M, Babić J, Jukić H, Banjari I. Bioactive profile of various Salvia officinalis L. Preparations. Plants (Basel). 2019;8: 55.

Llorens-Molina JA, Castel V, Vacas S, Verdeguer M. TLC-GC/MS Method for identifying and selecting valuable essential oil chemotypes from wild populations of Mentha longifolia L. Nat. Volatiles & Essent. Oils. 2017;4:49-61.