GC/MS Analysis of n-Hexane and Chloroform Extracts of Chenopodium murale Leaves in Iraq

Main Article Content

Muthanna Saadi Farhan
Amjed Haseeb Khamees
Omar Hussein Ahmed
Amani AmerTawfeeq
Yahya Saad Yaseen

Abstract

Chenopodium murale L. it is an essential annual herbaceous weed belongs to the genus Chenopodium and family Chenopodiaceae. Chenopodium murale L. commonly called as nettle leaf goosefoot. Aim of this study is the gas chromatography-mass spectroscopy analysis of chemical constituents of Chenopodium murale leaves in two different extracts; n-hexane and chloroform. These extracts contain 37 chemical components which are Monoterpenes, steroids precursor and fatty acids. Furthermore the n- hexane extract revealed about 35.22% of cyclic and acyclic monoterpenoids, fatty acid about 2.07%, also 2.31% of nitrogenous compounds and sterol precursor about 0.41%.

However the chloroform extract revealed the presence of linolenic acid representing 13.54% and neo menthol representing 18.87%, also the other minor components are carvone oxide (0.27%), alpha- pinene epoxide (1.71%), Trans- Squalene (0.77%) and other minor bioactive components.

Keywords:
GC/MS analysis, n-hexane, chloroform extracts, Chenopodium murale leaves.

Article Details

How to Cite
Saadi Farhan, M., Haseeb Khamees, A., Hussein Ahmed, O., AmerTawfeeq, A., & Saad Yaseen, Y. (2019). GC/MS Analysis of n-Hexane and Chloroform Extracts of Chenopodium murale Leaves in Iraq. Journal of Pharmaceutical Research International, 31(6), 1-6. https://doi.org/10.9734/jpri/2019/v31i630325
Section
Original Research Article

References

Gilani AH. Trends in ethnopharmacology. Journal of Ethnopharmacology. 2005; 100(1-2):43-9.

Morteza-Semnani K. A review on Chenopodium botrys L.: Traditional uses, chemical composition and biological activities. Pharm Biomed Res [Internet]. 2015;1(2):1–9.
[Cited 2019 Aug 22]
Available:http://pbr.mazums.ac.ir

Smith BD. Eastern North America as an independent center of plant domestication. Proceedings of the National Academy of Sciences. 2006;103(33):12223-8.

Paliwal R. Phytochemical and Pharmaco-gnostical analysis on chenopodium album: A plant with immense antioxidant and nutritional potential. 2015;2(1).

Riaz B, Zahoor MK, Zahoor MA, Majeed HN, Javed I, Ahmad A, et al. Toxicity, phytochemical composition, and enzyme inhibitory activities of some indigenous weed plant extracts in fruit fly, Drosophila melanogaster. Evidence-based Complement Altern Med;2018.

Ahmad B, Jan Q. Phytochemical evaluation of Chenopodium murale Linn. Bashir Ahmad, Qasim Jan, Shumaila Bashir, Muhammad Iqbal Choudhary and Muhammad Nisar. Asian Journal of Plant Sciences. 2003;2(15-16):1072-8.

Davis PH. No Title [Internet]. Edinburgh University Press. Available:PH Davis - Flora of Turkey, 1965 - cabdirect.org. 1967;567.

Kokanova-Nedialkova Z, Nedialkov PT, Nikolov SD. The genus Chenopodium: Phytochemistry, ethnopharmacology and pharmacology. Pharmacognosy Reviews; 2009.

Dembitsky V, Shkrob I, Hanus LO. Ascaridole and related peroxides from the genus Chenopodium. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub; 2008.

Singh HP, Batish DR, Kohli RK, Mittal S, Yadav S. Chemical composition of essential oil from leaves of Chenopodium ambrosioides from Chandigarh, India. Chem Nat Compd; 2008.

Van Rompuy LLL, Zeevaart JAD. Are steroidal estrogens natural plant constituents, Phytochemistry. 1979;18(5): 863–5.

Salt TA, Adler JH. Diversity of sterol composition in the family chenopodiaceae. Lipids; 1985.

Mizui F, Kasai R, Ohtani K, Tanaka O. Saponins from Brans of Quinoa, Chenopodium quinoa Willd I. Chem Pharm Bull; 1988.

Hifnawy MS, Ammar HH, Kenawy SK, Zaki ME, Yossef AK, Awaad SA. Phyto-chemical and biological studies on alkaloidal content of some allergy producing plants growing in Egypt. Bull Fac Pharm; Cairo Univ; 1999.

Cutillo F, D’Abrosca B, DellaGreca M, Di Marino C, Golino A, Previtera L, et al. Cinnamic acid amides from Chenopodium album: Effects on seeds germination and plant growth. Phytochemistry; 2003.

Gohar AA, Maatooq GT, Niwa M. Two flavonoid glycosides from Chenopodium murale. Phytochemistry. 2000;53(2):299–303.

Ahmed Oh, Hamad Mn, Jaafar Ns. Phyto-chemical investigation of Chenopodium Murale (Family: Chenopodiaceae) cultivated In Iraq, isolation and identification of Scopoletin and Gallic acid. Asian J Pharm Clin Res. 2017;10(11):70-7.

Yadav N, Vasudeva N, Singh S, Sharma SK. Medicinal properties of Genus chenopodium linn. Indian J Nat Prod Resour; 2007.

Ibrahim LF, Kawashty SA, Baiuomy AR, Shabana MM, El-Eraky WI, El-Negoumy SI. A comparative study of the flavonoids and some biological activities of two Chenopodium species. Chem Nat Compd; 2007.

Abdel-Aziz MS, Shaheen MS, El-Nekeety AA, Abdel-Wahhab MA. Antioxidant and antibacterial activity of silver nanoparticles biosynthesized using Chenopodium murale leaf extract. J Saudi Chem Soc [Internet]. 2014;18(4):356–63.
Available:http://dx.doi.org/10.1016/j.jscs.2013.09.011

Chludil HD, Corbino GB, Leicach SR. Soil quality effects on Chenopodium album flavonoid content and antioxidant potential. J Agric Food Chem; 2008.

Nowak R, Szewczyk K, Gawlik-Dziki U, Rzymowska J, Komsta Ł. Antioxidative and cytotoxic potential of some Chenopodium L. species growing in Poland. Saudi J Biol Sci. 2016;23(1):15–23.

Okuyama E, Umeyama K, Saito Y, Yamazaki M, Satake M. Ascaridole as a pharmacologically active principle of Paico, a medicinal peruvian plant. Chem Pharm Bull; 1993.

García R, Lemus I, Rivera P, Erazo S. Biological and chemical study of paico (Chenopodium chilense, Chenopodiaceae). J Ethnopharmacol; 1997.

Santos FA, Rao VSN. Antiinflammatory and antinociceptive effects of 1,8-cineole a terpenoid oxide present in many plant essential oils. Phyther Res; 2000.

Tawfeeq AA, Mahdi MF, Abaas IS, Alwan AH. Phytochemical and anti-bacterial studies of leaves of Rosmarinus officinalis cultivated in Karbala, Iraq. Al-Mustansiriyah Journal for Pharmaceutical Sciences. 2017;17(2):86-94.

de Sousa DP, Quintans L, de Almeida RN. Evolution of the anticonvulsant activity of α-terpineol. Pharm Biol [Internet]. 2007; 45(1):69–70.
Available:http://www.tandfonline.com/doi/full/10.1080/13880200601028388

Wu X, Li X, Xiao F, Zhang Z, Xu Z, Wang H. Studies on the analgesic and anti-inflammatory effect of bornyl acetate in volatile oil from Amomum villosum. Zhong Yao Cai; 2004.

Tawfeeq AA, Mahdi MF, Abaas IS, Alwan AH. Isolation, quantification and identifi-cation of rosmarinic acid, gas chromato-graphy-mass spectrometry analysis of essential oil, cytotoxic effect and antimicrobial investigation of rosmarinus officinalis leaves. Asian J Pharm Clin Res. 2018;11(6):126–32.

De Cássia Da Silveira E Sá R, Andrade LN, De Sousa DP. A review on anti-inflammatory activity of monoterpenes. Molecules. 2013;18.

Chaudhary J, Jain A, Kaur N, Kishore L. Stigmasterol: A comprehensive review; 2011.