The Effect of Ellagic Acid on Paraoxanase - 1 Activity and DNA Damage in Acute Exercise

Main Article Content

Ersan Kara
Funda Karabağ Çoban
Mustafa Akıl
Recep Liman
İbrahim Hakkı Ciğerci

Abstract

The purpose of this study was to evaluate the correlation between exhaustive and intensive exercise with changes in paraoxonase-1 enzyme activity, oxidative DNA damage and the role of ellagic acid against possible damage.

The study was carried out on 32 male and adult Spraque - Dawley rats at the Experimental Animal Research and Research Center of Afyon Kocatepe University. The experimental animals were equally divided into four groups. Swimming exercises were performed as acute exercises for once and experimental animals are made to swim in groups including two rats following the completion of the study and before the decapitation.

At the end of the experiment, obtained blood samples; Paraoxonase-1 (PON-1), Malondialdehyde (MDA) and 8-HydroxyGuanine (8-OhdG) levels were measured to determine DNA damage and DNA damage was assessed by Comet Assay method.

As a result, PON-1 levels in rats with intense swimming training were found to be significantly lower (p <0.05) than the control group. MDA and (8-OhdG) levels were significantly higher in the swimming group than in the control group (p <0.05). As to the DNA damage determination by COMET analysis, DNA damage was observed in the swimming groups according to the control groups. When the ellagic acid groups were compared with the swimming groups, there was a significant increase in PON-1 levels, and the levels of MDA and (8-OhdG) were significantly lower than the swimming groups. The DNA damage was also found to be low in these groups.

Keywords:
Exercise, ellagic acid, PON-1, DNA damage

Article Details

How to Cite
Kara, E., Çoban, F. K., Akıl, M., Liman, R., & Ciğerci, İbrahim H. (2019). The Effect of Ellagic Acid on Paraoxanase - 1 Activity and DNA Damage in Acute Exercise. Journal of Pharmaceutical Research International, 29(5), 1-7. https://doi.org/10.9734/jpri/2019/v29i530250
Section
Original Research Article

References

Abed KE, Rebai H, Bloomer RJ, Trabelsi K, Masmoudi L, Zbidi A, Sahnoun Z, Hakim A, Tabka Z. Antioksidant status and oksidative stres at rest and in response to acute exercise in judokas and sedantary men. Journal of Strength & Conditioning Research. 2011;25(9):2400-2409.

Jakesevic M, Aaby K, Borge GIA, Jeppsson B, Ahrne S, Molin G. Antioxidative protection of dietary bilberry, chokeberry and Lactobacillus plantarum HEAL19 in mice subjected to intestinal oxidative stress by ischemia-reperfusion. BMC Complementary and Alternative Medicine. 2011;27:8-11.

Bloomer RJ, Goldfarb AH. Anaerobic exercise and oxidative stres. Canadian Journal of Applied Physiology. 2004;29: 245-63.

Boveris A, Navarro A. Systemic and mitochondrial adaptive responsest moderate exercise in rodents. Free Radical Biology and Medicine. 2008;44:224-229.

Leonardo Sepúlveda, Alberto Ascacio, Raúl Rodríguez-Herrera, Antonio Aguilera-Carbó and Cristóbal N. Aguilar. Ellagic acid: Biological properties and biotechno-logical development for production processes. African Journal of Biotechno-logy. 2011;10(22):4518-4523.

Dawson Ca, Horvarth SM. Swimming in small laboratory animals. Medicine and Science in Sports. 1970;2:51-78.

Draper HH, Hardley M. Malondialdehydedetermination as index of lipid peroxidation. Methods Enzymol. 1990; 186:421-31.

Kocyigit A, Keles H, Selek S, Guzel S, Celik H, Erel O. Increased DNA damage and oxidative stress in patients with Cutaneous Leishmaniasis. Mutat Res. 2005;585:71-78.

Aguilo A, Tauler P, Pilar Guix M, Villa G, Cordova A, Tur JA, Pons A. Effect of exercise intensity and training on antioxidants and cholesterol profile in cyclists. The Journal of Nutritional Biochemistry. 2003;14:319-325.

Tekcan M. Oksidatif stres-antioksidan sistemler. Infertilite, Androloji Bülteni. 2009; 131-136.

Radak Z, Sasvari M, Nyakas C, Pucsok J, Nakamoto H, Goto S. Exercise precondi-tioning against hydrogen peroxide induced oxidative damage in proteins of rat myocardium. Archives of Biochemistry and Biophysics. 2000;376:248–251.

Atalay M, Oksala NK, Laaksonen DE, Savita Khanna, Chitose Nakao, Jani Lappalainen, Sashwati Roy, Osmo Hanninen, Sen CK. Exercise training-modulates heat shock protein response in diabetic rats. Journal of Applied Physiology. 2004;97:605-611.

Banerjee AK, Mandal A, Chanda D, Chakraborti S. Oxidant, antioxidant and physical exercise. Molecular and Cellular Biochemistry. 2003;253:307-312.

White A, Estrada M, Walker, K, Wisnia P. Filgueira G, Valdes F, Araneda O, Behn C, Martinez R. Role of exercise and ascorbate on plasma antioxidant capacity in thoroughbred race horses. Comparative Biochemistry and Physiology, Part A Molecular and Integrative Physiology. 2001;128:99-104.

Timothy IM, Kevin EE, Hageman KS, Poole DC. Altered regional blood flow responses to submaximal exercise in older rats. Journal of Applied Physiology. 2003; 96:81–88.

Kalinowska BR, Korczala KZ, Kalinowski M, Kukla M, Birkner E, Jochem J. Activity of antioxidative enzymes and concentration of malondialdehyde as oxidative status markers in women with newly diagnosed Graves Basedow disease and after thiamazole therapy leading to euthyroidism. Archıwum Medycyny Wewnętrznej. 2008;118:7-8.

Şemin I, Kayatekin BM, Gönenç S, Açıkgöz O, Uysal N, Delen Y, Güre A. Effect of one hour of exercise on antipyretic enzymes and peroxidation of small intestine, kidney and muscle tissue in anthropene mice. Hacettepe Journal Sport Science. 1998;4(9):33-40.

Taş M. The effect of speed exercises on serum superoxide dismutase, catalase and malondialdehyde levels in football players. Health Sciences Institute, Department of Physical Education and Sports (Master), Erzurum: Atatürk University; 2006.

Alessio HMHA, Fulkerson BK, Ambrose J, Rice RE, Wiley RL. Generation of reactive oxygen species after exhaustive aerobic and ısometric exercise medicine science, sports exercise. 2000;32(9):1576-1581.

Duffaux B, Heine O, Kothe A, Prinz U, Rost R. Blood glutation status follovving distance running. Internationel Journal Sports Medicine. 1997;18:89-93.

Leaf DA. The effect of exercise intensity on lipid peroxidation. Medicine Scients Sports Exercise. 1997;29(8):1106–1109.

Grisham MB. Reactive meabolites of oxygen and nitrogen in biology and medicine. RG Landes Company. 1992;5-28.

Dernbach AR, Sherman WM, Simonsen JC, Flowers KM, Lamb DR. No evidence of oxidant stress during high ıntensity rowing training. Journal of Applied Physiology, 1993;74(5):2140-2145.

Selamoğlu S. Effects of aerobic and anaerobic training on the defense system of athletes. Health Sciences Institute (PhD), İzmir: Dokuz Eylül University; 2000.

Çelik A, Varol R, Onat T, Dağdelen Y, Tugay F. Effect of acute exercise on antioxidant system parameters in football players. Journal of Physical Education and Sports Sciences. 2007;4:167-172.

Williams KJ, Tabas I. The response-to-retention hypothesis of early athero-genesis. Arterioscler ThrombB Vasc Biol. 1995;15:551–61.

Berliner JA, Navab M, Fogelman AM, et al. Atherosclerosis: Basic mechanisms. Oxidation, inflammation, and genetics. Circulation. 1995;91:2488–96.

Funda Karabag Coban, Mustafa Akil, Recep Liman, İbrahim Ciğerci. Effects of resveratrol on oxidative DNA damage-ınduced by the acute swimming exercise. Journal of Pharmaceutical Research International. 2018;21(4):1-10.

Fıçıcılar H, Zergeroğlu AM, Tekin D, Ersöz G. The effects of acute exercise on plasma antioxidant status and platelet response. Thrombosis Research. 2003;111:267- 271.

Khanduja KL, Gandhi RK, Pathania V, Syal N. Prevention of N nitrosodiethylamine-induced lung tumorigenesis by ellagic acid and quercetin in mice. Food and Chemical Toxicology. 1999;37:313-318.

Akkuş D. Free radicals and their physiopathological effects. Mimoza Publications, Konya; 1995.

Rodrigo R, Rivera G, Orellana M, Araya J, Bosco C. Rat kidney antioxidant response to long-term exposure to flavonol rich red wine. Life Sci. 2002;71(24):2881- 2895.